Ness JE - Search Results

1

Engineering proteinase K using machine learning and synthetic genes.

Liao J, Warmuth MK, Govindarajan S, Ness JE, Wang RP, Gustafsson C, Minshull J. Liao J, et al. Among authors: ness je. BMC Biotechnol. 2007 Mar 26;7:16. doi: 10.1186/1472-6750-7-16. BMC Biotechnol. 2007. PMID: 17386103 Free PMC article.

2

Engineering genes for predictable protein expression.

Gustafsson C, Minshull J, Govindarajan S, Ness J, Villalobos A, Welch M. Gustafsson C, et al. Protein Expr Purif. 2012 May;83(1):37-46. doi: 10.1016/j.pep.2012.02.013. Epub 2012 Mar 8. Protein Expr Purif. 2012. PMID: 22425659 Free PMC article. Review.

3

Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently.

Ness JE, Kim S, Gottman A, Pak R, Krebber A, Borchert TV, Govindarajan S, Mundorff EC, Minshull J. Ness JE, et al. Nat Biotechnol. 2002 Dec;20(12):1251-5. doi: 10.1038/nbt754. Epub 2002 Nov 11. Nat Biotechnol. 2002. PMID: 12426575

4

Engineered protein function by selective amino acid diversification.

Minshull J, Govindarajan S, Cox T, Ness JE, Gustafsson C. Minshull J, et al. Among authors: ness je. Methods. 2004 Apr;32(4):416-27. doi: 10.1016/j.ymeth.2003.10.004. Methods. 2004. PMID: 15003604

5

Gene Designer: a synthetic biology tool for constructing artificial DNA segments.

Villalobos A, Ness JE, Gustafsson C, Minshull J, Govindarajan S. Villalobos A, et al. Among authors: ness je. BMC Bioinformatics. 2006 Jun 6;7:285. doi: 10.1186/1471-2105-7-285. BMC Bioinformatics. 2006. PMID: 16756672 Free PMC article.

6

Design parameters to control synthetic gene expression in Escherichia coli.

Welch M, Govindarajan S, Ness JE, Villalobos A, Gurney A, Minshull J, Gustafsson C. Welch M, et al. Among authors: ness je. PLoS One. 2009 Sep 14;4(9):e7002. doi: 10.1371/journal.pone.0007002. PLoS One. 2009. PMID: 19759823 Free PMC article.

7

Predicting enzyme function from protein sequence.

Minshull J, Ness JE, Gustafsson C, Govindarajan S. Minshull J, et al. Among authors: ness je. Curr Opin Chem Biol. 2005 Apr;9(2):202-9. doi: 10.1016/j.cbpa.2005.02.003. Curr Opin Chem Biol. 2005. PMID: 15811806 Review.

8

DNA shuffling of subgenomic sequences of subtilisin.

Ness JE, Welch M, Giver L, Bueno M, Cherry JR, Borchert TV, Stemmer WP, Minshull J. Ness JE, et al. Nat Biotechnol. 1999 Sep;17(9):893-6. doi: 10.1038/12884. Nat Biotechnol. 1999. PMID: 10471932

9

Systematic variation of amino acid substitutions for stringent assessment of pairwise covariation.

Govindarajan S, Ness JE, Kim S, Mundorff EC, Minshull J, Gustafsson C. Govindarajan S, et al. Among authors: ness je. J Mol Biol. 2003 May 16;328(5):1061-9. doi: 10.1016/s0022-2836(03)00357-7. J Mol Biol. 2003. PMID: 12729741

10

Evolutionary potential of (beta/alpha)8-barrels: functional promiscuity produced by single substitutions in the enolase superfamily.

Schmidt DM, Mundorff EC, Dojka M, Bermudez E, Ness JE, Govindarajan S, Babbitt PC, Minshull J, Gerlt JA. Schmidt DM, et al. Among authors: ness je. Biochemistry. 2003 Jul 22;42(28):8387-93. doi: 10.1021/bi034769a. Biochemistry. 2003. PMID: 12859183

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