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A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies.
Ng ES, Davis R, Stanley EG, Elefanty AG. Ng ES, et al. Nat Protoc. 2008;3(5):768-76. doi: 10.1038/nprot.2008.42. Nat Protoc. 2008. PMID: 18451785
The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells.
Ng ES, Azzola L, Sourris K, Robb L, Stanley EG, Elefanty AG. Ng ES, et al. Development. 2005 Mar;132(5):873-84. doi: 10.1242/dev.01657. Epub 2005 Jan 26. Development. 2005. PMID: 15673572
The hESC line Envy expresses high levels of GFP in all differentiated progeny.
Costa M, Dottori M, Ng E, Hawes SM, Sourris K, Jamshidi P, Pera MF, Elefanty AG, Stanley EG. Costa M, et al. Nat Methods. 2005 Apr;2(4):259-60. doi: 10.1038/nmeth748. Epub 2005 Mar 23. Nat Methods. 2005. PMID: 15782217
Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation.
Ng ES, Davis RP, Azzola L, Stanley EG, Elefanty AG. Ng ES, et al. Blood. 2005 Sep 1;106(5):1601-3. doi: 10.1182/blood-2005-03-0987. Epub 2005 May 24. Blood. 2005. PMID: 15914555
Mixl1 and oct4 proteins are transiently co-expressed in differentiating mouse and human embryonic stem cells.
Mossman AK, Sourris K, Ng E, Stanley EG, Elefanty AG. Mossman AK, et al. Stem Cells Dev. 2005 Dec;14(6):656-63. doi: 10.1089/scd.2005.14.656. Stem Cells Dev. 2005. PMID: 16433620
Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene.
Hirst CE, Ng ES, Azzola L, Voss AK, Thomas T, Stanley EG, Elefanty AG. Hirst CE, et al. Among authors: ng es. Dev Biol. 2006 May 1;293(1):90-103. doi: 10.1016/j.ydbio.2006.01.023. Epub 2006 Mar 20. Dev Biol. 2006. PMID: 16546155
Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors.
Davis RP, Ng ES, Costa M, Mossman AK, Sourris K, Elefanty AG, Stanley EG. Davis RP, et al. Among authors: ng es. Blood. 2008 Feb 15;111(4):1876-84. doi: 10.1182/blood-2007-06-093609. Epub 2007 Nov 21. Blood. 2008. PMID: 18032708
Directed differentiation of human embryonic stem cells as spin embryoid bodies and a description of the hematopoietic blast colony forming assay.
Ng ES, Davis RP, Hatzistavrou T, Stanley EG, Elefanty AG. Ng ES, et al. Curr Protoc Stem Cell Biol. 2008 Jan;Chapter 1:Unit 1D.3. doi: 10.1002/9780470151808.sc01d03s4. Curr Protoc Stem Cell Biol. 2008. PMID: 18770631
ErythRED, a hESC line enabling identification of erythroid cells.
Hatzistavrou T, Micallef SJ, Ng ES, Vadolas J, Stanley EG, Elefanty AG. Hatzistavrou T, et al. Among authors: ng es. Nat Methods. 2009 Sep;6(9):659-62. doi: 10.1038/nmeth.1364. Epub 2009 Aug 23. Nat Methods. 2009. PMID: 19701193
Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells.
Heraud P, Ng ES, Caine S, Yu QC, Hirst C, Mayberry R, Bruce A, Wood BR, McNaughton D, Stanley EG, Elefanty AG. Heraud P, et al. Among authors: ng es. Stem Cell Res. 2010 Mar;4(2):140-7. doi: 10.1016/j.scr.2009.11.002. Epub 2009 Dec 2. Stem Cell Res. 2010. PMID: 20060373
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