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Electrophysiological patch clamp assay to monitor the action of opioid receptors.
Spahn V, Nockemann D, Machelska H. Spahn V, et al. Among authors: nockemann d. Methods Mol Biol. 2015;1230:197-211. doi: 10.1007/978-1-4939-1708-2_16. Methods Mol Biol. 2015. PMID: 25293327
The K(+) channel GIRK2 is both necessary and sufficient for peripheral opioid-mediated analgesia.
Nockemann D, Rouault M, Labuz D, Hublitz P, McKnelly K, Reis FC, Stein C, Heppenstall PA. Nockemann D, et al. EMBO Mol Med. 2013 Aug;5(8):1263-77. doi: 10.1002/emmm.201201980. Epub 2013 Jul 1. EMBO Mol Med. 2013. PMID: 23818182 Free PMC article.
Analysis of potassium and calcium imaging to assay the function of opioid receptors.
Spahn V, Nockemann D, Machelska H. Spahn V, et al. Among authors: nockemann d. Methods Mol Biol. 2015;1230:187-96. doi: 10.1007/978-1-4939-1708-2_15. Methods Mol Biol. 2015. PMID: 25293326
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