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Homogeneous, bioluminescent proteasome assays.
O'Brien MA, Moravec RA, Riss TL, Bulleit RF. O'Brien MA, et al. Methods Mol Biol. 2008;414:163-81. doi: 10.1007/978-1-59745-339-4_13. Methods Mol Biol. 2008. PMID: 18175819 Review.
Homogeneous, bioluminescent proteasome assays.
O'Brien MA, Moravec RA, Riss TL, Bulleit RF. O'Brien MA, et al. Methods Mol Biol. 2015;1219:95-114. doi: 10.1007/978-1-4939-1661-0_9. Methods Mol Biol. 2015. PMID: 25308265
A bioluminescent assay for the sensitive detection of proteases.
Leippe DM, Nguyen D, Zhou M, Good T, Kirkland TA, Scurria M, Bernad L, Ugo T, Vidugiriene J, Cali JJ, Klaubert DH, O'Brien MA. Leippe DM, et al. Biotechniques. 2011 Aug;51(2):105-10. doi: 10.2144/000113716. Biotechniques. 2011. PMID: 21806554
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format.
Moravec RA, O'Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL. Moravec RA, et al. Anal Biochem. 2009 Apr 15;387(2):294-302. doi: 10.1016/j.ab.2009.01.016. Epub 2009 Jan 19. Anal Biochem. 2009. PMID: 19454251
Bioluminescent assays for ADMET.
Cali JJ, Niles A, Valley MP, O'Brien MA, Riss TL, Shultz J. Cali JJ, et al. Expert Opin Drug Metab Toxicol. 2008 Jan;4(1):103-20. doi: 10.1517/17425255.4.1.103. Expert Opin Drug Metab Toxicol. 2008. PMID: 18370862 Review.
Homogeneous, bioluminescent protease assays: caspase-3 as a model.
O'Brien MA, Daily WJ, Hesselberth PE, Moravec RA, Scurria MA, Klaubert DH, Bulleit RF, Wood KV. O'Brien MA, et al. J Biomol Screen. 2005 Mar;10(2):137-48. doi: 10.1177/1087057104271865. J Biomol Screen. 2005. PMID: 15799957
Poly (ADP-ribose) polymerase cleavage monitored in situ in apoptotic cells.
O'Brien MA, Moravec RA, Riss TL. O'Brien MA, et al. Biotechniques. 2001 Apr;30(4):886-91. doi: 10.2144/01304pf01. Biotechniques. 2001. PMID: 11314271
Self-immolative bioluminogenic quinone luciferins for NAD(P)H assays and reducing capacity-based cell viability assays.
Zhou W, Leippe D, Duellman S, Sobol M, Vidugiriene J, O'Brien M, Shultz JW, Kimball JJ, DiBernardo C, Moothart L, Bernad L, Cali J, Klaubert DH, Meisenheimer P. Zhou W, et al. Chembiochem. 2014 Mar 21;15(5):670-5. doi: 10.1002/cbic.201300744. Epub 2014 Mar 3. Chembiochem. 2014. PMID: 24591148
A bioluminescent caspase-1 activity assay rapidly monitors inflammasome activation in cells.
O'Brien M, Moehring D, Muñoz-Planillo R, Núñez G, Callaway J, Ting J, Scurria M, Ugo T, Bernad L, Cali J, Lazar D. O'Brien M, et al. J Immunol Methods. 2017 Aug;447:1-13. doi: 10.1016/j.jim.2017.03.004. Epub 2017 Mar 6. J Immunol Methods. 2017. PMID: 28268194
Single Step Fluorescent Caspase 3 Activity Assay: Quantifying the Inhibition of Cell Permeable Compounds in a Cell-Based System.
Humpal-Winter J, Niles A, OBrien M, Bulleit B. Humpal-Winter J, et al. ScientificWorldJournal. 2001 Nov 27;1:135. doi: 10.1100/tsw.2001.236. eCollection 2001. ScientificWorldJournal. 2001. PMID: 30147613 Free PMC article. No abstract available.
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