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Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA.
Olmos A, Cambra M, Dasi MA, Candresse T, Esteban O, Gorris MT, Asensio M. Olmos A, et al. J Virol Methods. 1997 Nov;68(2):127-37. doi: 10.1016/s0166-0934(97)00120-1. J Virol Methods. 1997. PMID: 9389402
Ten PPV isolates typed using PPV-D and PPV-M specific monoclonal antibodies by ELISA-DASI were used to validate these two methods. The results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. ...Testing of 228 PPV-infecte …
Ten PPV isolates typed using PPV-D and PPV-M specific monoclonal antibodies by ELISA-DASI were used to validate these two methods. The resul …
Nested rt-PCR in a single closed tube.
Olmos A, Esteban O, Bertolini E, Cambra M. Olmos A, et al. Methods Mol Biol. 2003;226:151-60. doi: 10.1385/1-59259-384-4:151. Methods Mol Biol. 2003. PMID: 12958495 No abstract available.
212 results