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The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. Götzke H, et al. Among authors: opazo f. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. Nat Commun. 2019. PMID: 31562305 Free PMC article.
Resolving bundled microtubules using anti-tubulin nanobodies.
Mikhaylova M, Cloin BM, Finan K, van den Berg R, Teeuw J, Kijanka MM, Sokolowski M, Katrukha EA, Maidorn M, Opazo F, Moutel S, Vantard M, Perez F, van Bergen en Henegouwen PM, Hoogenraad CC, Ewers H, Kapitein LC. Mikhaylova M, et al. Among authors: opazo f. Nat Commun. 2015 Aug 11;6:7933. doi: 10.1038/ncomms8933. Nat Commun. 2015. PMID: 26260773 Free PMC article.
Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F. Sograte-Idrissi S, et al. Among authors: opazo f. Nanoscale. 2020 May 14;12(18):10226-10239. doi: 10.1039/d0nr00227e. Nanoscale. 2020. PMID: 32356544
A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer B, Strohäker T, Zweckstetter M, Becker S, Rizzoli SO, Basmanav FB, Opazo F. Gerdes C, et al. Among authors: opazo f. Nat Commun. 2020 Jun 1;11(1):2729. doi: 10.1038/s41467-020-16575-0. Nat Commun. 2020. PMID: 32483166 Free PMC article.
60 results