Genome sequence of the Ornithopus/Lupinus-nodulating Bradyrhizobium sp. strain WSM471

Wayne Reeve; Sofie De Meyer; Jason Terpolilli; Vanessa Melino; Julie Ardley; Rui Tian; Ravi Tiwari; John Howieson; Ronald Yates; Graham O'Hara; Mohamed Ninawi; Megan Lu; David Bruce; Chris Detter; Roxanne Tapia; Cliff Han; Chia-Lin Wei; Marcel Huntemann; James Han; I-Min Chen; Konstantinos Mavromatis; Victor Markowitz; Natalia Ivanova; Ioanna Pagani; Amrita Pati; Lynne Goodwin; Tanja Woyke; Nikos Kyrpides

doi:10.4056/sigs.4498256

Genome sequence of the Ornithopus/Lupinus-nodulating Bradyrhizobium sp. strain WSM471

Stand Genomic Sci. 2013 Dec 15;9(2):254-63. doi: 10.4056/sigs.4498256. eCollection 2013 Dec 20.

Authors

Wayne Reeve¹, Sofie De Meyer¹, Jason Terpolilli¹, Vanessa Melino¹, Julie Ardley¹, Rui Tian¹, Ravi Tiwari¹, John Howieson¹, Ronald Yates², Graham O'Hara¹, Mohamed Ninawi¹, Megan Lu³, David Bruce³, Chris Detter³, Roxanne Tapia³, Cliff Han³, Chia-Lin Wei³, Marcel Huntemann³, James Han³, I-Min Chen⁴, Konstantinos Mavromatis³, Victor Markowitz⁴, Natalia Ivanova³, Ioanna Pagani³, Amrita Pati³, Lynne Goodwin⁵, Tanja Woyke³, Nikos Kyrpides³

Affiliations

¹ Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
² Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia ; Department of Agriculture and Food, Western Australia, Australia.
³ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁴ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
⁵ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.

Abstract

Bradyrhizobium sp. strain WSM471 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen- (N2) fixing root nodule formed on the annual legume Ornithopus pinnatus (Miller) Druce growing at Oyster Harbour, Albany district, Western Australia in 1982. This strain is in commercial production as an inoculant for Lupinus and Ornithopus. Here we describe the features of Bradyrhizobium sp. strain WSM471, together with genome sequence information and annotation. The 7,784,016 bp high-quality-draft genome is arranged in 1 scaffold of 2 contigs, contains 7,372 protein-coding genes and 58 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.

Keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.