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Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta(DE3).
Tegel H, Tourle S, Ottosson J, Persson A. Tegel H, et al. Among authors: ottosson j. Protein Expr Purif. 2010 Feb;69(2):159-67. doi: 10.1016/j.pep.2009.08.017. Epub 2009 Sep 4. Protein Expr Purif. 2010. PMID: 19733669
Automated sample preparation method for mass spectrometry analysis on recombinant proteins.
Steen J, Ramström M, Uhlén M, Hober S, Ottosson J. Steen J, et al. Among authors: ottosson j. J Chromatogr A. 2009 May 15;1216(20):4457-64. doi: 10.1016/j.chroma.2009.03.041. Epub 2009 Mar 20. J Chromatogr A. 2009. PMID: 19345368
High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week.
Tegel H, Steen J, Konrad A, Nikdin H, Pettersson K, Stenvall M, Tourle S, Wrethagen U, Xu L, Yderland L, Uhlén M, Hober S, Ottosson J. Tegel H, et al. Among authors: ottosson j. Biotechnol J. 2009 Jan;4(1):51-7. doi: 10.1002/biot.200800183. Biotechnol J. 2009. PMID: 19039781
A general, robust method for the quality control of intact proteins using LC-ESI-MS.
Sundqvist G, Stenvall M, Berglund H, Ottosson J, Brumer H. Sundqvist G, et al. Among authors: ottosson j. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):188-94. doi: 10.1016/j.jchromb.2007.01.011. Epub 2007 Jan 18. J Chromatogr B Analyt Technol Biomed Life Sci. 2007. PMID: 17267305
Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling.
Nilsson P, Paavilainen L, Larsson K, Odling J, Sundberg M, Andersson AC, Kampf C, Persson A, Al-Khalili Szigyarto C, Ottosson J, Björling E, Hober S, Wernérus H, Wester K, Pontén F, Uhlen M. Nilsson P, et al. Among authors: ottosson j. Proteomics. 2005 Nov;5(17):4327-37. doi: 10.1002/pmic.200500072. Proteomics. 2005. PMID: 16237735
A human protein atlas for normal and cancer tissues based on antibody proteomics.
Uhlén M, Björling E, Agaton C, Szigyarto CA, Amini B, Andersen E, Andersson AC, Angelidou P, Asplund A, Asplund C, Berglund L, Bergström K, Brumer H, Cerjan D, Ekström M, Elobeid A, Eriksson C, Fagerberg L, Falk R, Fall J, Forsberg M, Björklund MG, Gumbel K, Halimi A, Hallin I, Hamsten C, Hansson M, Hedhammar M, Hercules G, Kampf C, Larsson K, Lindskog M, Lodewyckx W, Lund J, Lundeberg J, Magnusson K, Malm E, Nilsson P, Odling J, Oksvold P, Olsson I, Oster E, Ottosson J, Paavilainen L, Persson A, Rimini R, Rockberg J, Runeson M, Sivertsson A, Sköllermo A, Steen J, Stenvall M, Sterky F, Strömberg S, Sundberg M, Tegel H, Tourle S, Wahlund E, Waldén A, Wan J, Wernérus H, Westberg J, Wester K, Wrethagen U, Xu LL, Hober S, Pontén F. Uhlén M, et al. Among authors: ottosson j. Mol Cell Proteomics. 2005 Dec;4(12):1920-32. doi: 10.1074/mcp.M500279-MCP200. Epub 2005 Aug 27. Mol Cell Proteomics. 2005. PMID: 16127175
A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein.
Hedhammar M, Stenvall M, Lönneborg R, Nord O, Sjölin O, Brismar H, Uhlén M, Ottosson J, Hober S. Hedhammar M, et al. Among authors: ottosson j. J Biotechnol. 2005 Sep 23;119(2):133-46. doi: 10.1016/j.jbiotec.2005.03.024. J Biotechnol. 2005. PMID: 15996784
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