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Automated sample preparation method for mass spectrometry analysis on recombinant proteins.
Steen J, Ramström M, Uhlén M, Hober S, Ottosson J. Steen J, et al. Among authors: ottosson j. J Chromatogr A. 2009 May 15;1216(20):4457-64. doi: 10.1016/j.chroma.2009.03.041. Epub 2009 Mar 20. J Chromatogr A. 2009. PMID: 19345368
Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta(DE3).
Tegel H, Tourle S, Ottosson J, Persson A. Tegel H, et al. Among authors: ottosson j. Protein Expr Purif. 2010 Feb;69(2):159-67. doi: 10.1016/j.pep.2009.08.017. Epub 2009 Sep 4. Protein Expr Purif. 2010. PMID: 19733669
High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week.
Tegel H, Steen J, Konrad A, Nikdin H, Pettersson K, Stenvall M, Tourle S, Wrethagen U, Xu L, Yderland L, Uhlén M, Hober S, Ottosson J. Tegel H, et al. Among authors: ottosson j. Biotechnol J. 2009 Jan;4(1):51-7. doi: 10.1002/biot.200800183. Biotechnol J. 2009. PMID: 19039781
A general, robust method for the quality control of intact proteins using LC-ESI-MS.
Sundqvist G, Stenvall M, Berglund H, Ottosson J, Brumer H. Sundqvist G, et al. Among authors: ottosson j. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):188-94. doi: 10.1016/j.jchromb.2007.01.011. Epub 2007 Jan 18. J Chromatogr B Analyt Technol Biomed Life Sci. 2007. PMID: 17267305
Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling.
Nilsson P, Paavilainen L, Larsson K, Odling J, Sundberg M, Andersson AC, Kampf C, Persson A, Al-Khalili Szigyarto C, Ottosson J, Björling E, Hober S, Wernérus H, Wester K, Pontén F, Uhlen M. Nilsson P, et al. Among authors: ottosson j. Proteomics. 2005 Nov;5(17):4327-37. doi: 10.1002/pmic.200500072. Proteomics. 2005. PMID: 16237735
A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein.
Hedhammar M, Stenvall M, Lönneborg R, Nord O, Sjölin O, Brismar H, Uhlén M, Ottosson J, Hober S. Hedhammar M, et al. Among authors: ottosson j. J Biotechnol. 2005 Sep 23;119(2):133-46. doi: 10.1016/j.jbiotec.2005.03.024. J Biotechnol. 2005. PMID: 15996784
High-throughput solubility assay for purified recombinant protein immunogens.
Stenvall M, Steen J, Uhlén M, Hober S, Ottosson J. Stenvall M, et al. Among authors: ottosson j. Biochim Biophys Acta. 2005 Aug 31;1752(1):6-10. doi: 10.1016/j.bbapap.2005.07.002. Biochim Biophys Acta. 2005. PMID: 16084133
Enhancing the protein production levels in Escherichia coli with a strong promoter.
Tegel H, Ottosson J, Hober S. Tegel H, et al. Among authors: ottosson j. FEBS J. 2011 Mar;278(5):729-39. doi: 10.1111/j.1742-4658.2010.07991.x. Epub 2011 Jan 12. FEBS J. 2011. PMID: 21205203
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