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Automated sample preparation method for mass spectrometry analysis on recombinant proteins.
Steen J, Ramström M, Uhlén M, Hober S, Ottosson J. Steen J, et al. J Chromatogr A. 2009 May 15;1216(20):4457-64. doi: 10.1016/j.chroma.2009.03.041. Epub 2009 Mar 20. J Chromatogr A. 2009. PMID: 19345368
High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week.
Tegel H, Steen J, Konrad A, Nikdin H, Pettersson K, Stenvall M, Tourle S, Wrethagen U, Xu L, Yderland L, Uhlén M, Hober S, Ottosson J. Tegel H, et al. Biotechnol J. 2009 Jan;4(1):51-7. doi: 10.1002/biot.200800183. Biotechnol J. 2009. PMID: 19039781
A genecentric Human Protein Atlas for expression profiles based on antibodies.
Berglund L, Björling E, Oksvold P, Fagerberg L, Asplund A, Szigyarto CA, Persson A, Ottosson J, Wernérus H, Nilsson P, Lundberg E, Sivertsson A, Navani S, Wester K, Kampf C, Hober S, Pontén F, Uhlén M. Berglund L, et al. Mol Cell Proteomics. 2008 Oct;7(10):2019-27. doi: 10.1074/mcp.R800013-MCP200. Mol Cell Proteomics. 2008. PMID: 18669619 Review.
A general, robust method for the quality control of intact proteins using LC-ESI-MS.
Sundqvist G, Stenvall M, Berglund H, Ottosson J, Brumer H. Sundqvist G, et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):188-94. doi: 10.1016/j.jchromb.2007.01.011. Epub 2007 Jan 18. J Chromatogr B Analyt Technol Biomed Life Sci. 2007. PMID: 17267305
A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein.
Hedhammar M, Stenvall M, Lönneborg R, Nord O, Sjölin O, Brismar H, Uhlén M, Ottosson J, Hober S. Hedhammar M, et al. J Biotechnol. 2005 Sep 23;119(2):133-46. doi: 10.1016/j.jbiotec.2005.03.024. J Biotechnol. 2005. PMID: 15996784
High-throughput solubility assay for purified recombinant protein immunogens.
Stenvall M, Steen J, Uhlén M, Hober S, Ottosson J. Stenvall M, et al. Biochim Biophys Acta. 2005 Aug 31;1752(1):6-10. doi: 10.1016/j.bbapap.2005.07.002. Biochim Biophys Acta. 2005. PMID: 16084133
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