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Ultra wide-field lens-free monitoring of cells on-chip.
Ozcan A, Demirci U. Ozcan A, et al. Lab Chip. 2008 Jan;8(1):98-106. doi: 10.1039/b713695a. Epub 2007 Nov 1. Lab Chip. 2008. PMID: 18094767
This technology can monitor/count cells over a field-of-view that is more than two orders of magnitude larger than that of a conventional light microscope. ...An important application of this approach could be a miniaturized point-of-care technology to obtain …
This technology can monitor/count cells over a field-of-view that is more than two orders of magnitude larger than that of a c …
High-throughput lensfree imaging and characterization of a heterogeneous cell solution on a chip.
Su TW, Seo S, Erlinger A, Ozcan A. Su TW, et al. Among authors: ozcan a. Biotechnol Bioeng. 2009 Feb 15;102(3):856-868. doi: 10.1002/bit.22116. Biotechnol Bioeng. 2009. PMID: 18853435 Free PMC article.
A high-throughput on-chip imaging platform that can rapidly monitor and characterize various cell types within a heterogeneous solution over a depth-of-field of approximately 4 mm and a field-of-view of approximately 10 cm(2) is introduced. This powerf
A high-throughput on-chip imaging platform that can rapidly monitor and characterize various cell types within a heterogeneous
Multi-angle LUCAS for high-throughput on-chip cytometry.
Su TW, Seo S, Erlinger A, Ozcan A. Su TW, et al. Among authors: ozcan a. Annu Int Conf IEEE Eng Med Biol Soc. 2008;2008:1854-5. doi: 10.1109/IEMBS.2008.4649543. Annu Int Conf IEEE Eng Med Biol Soc. 2008. PMID: 19163046
We illustrate that by recording under-sampled diffraction patterns of cells at different illumination angles, we can achieve high-throughput on-chip characterization of a heterogeneous cell solution over an ultra large volume of approximately 5 ml. ...
We illustrate that by recording under-sampled diffraction patterns of cells at different illumination angles, we can achieve high-throughput …
Lensfree holographic imaging for on-chip cytometry and diagnostics.
Seo S, Su TW, Tseng DK, Erlinger A, Ozcan A. Seo S, et al. Among authors: ozcan a. Lab Chip. 2009 Mar 21;9(6):777-87. doi: 10.1039/b813943a. Epub 2008 Dec 5. Lab Chip. 2009. PMID: 19255659 Free PMC article.
We experimentally illustrate a lensfree holographic imaging platform to perform on-chip cytometry. By controlling the spatial coherence of the illumination source, we record a 2D holographic diffraction pattern of each cell or micro-particle on a chip using …
We experimentally illustrate a lensfree holographic imaging platform to perform on-chip cytometry. By controlling the spatial coheren …
Integrating microfluidics and lensless imaging for point-of-care testing.
Moon S, Keles HO, Ozcan A, Khademhosseini A, Haeggstrom E, Kuritzkes D, Demirci U. Moon S, et al. Among authors: ozcan a. Biosens Bioelectron. 2009 Jul 15;24(11):3208-14. doi: 10.1016/j.bios.2009.03.037. Epub 2009 Apr 2. Biosens Bioelectron. 2009. PMID: 19467854 Free PMC article.
Gray scale image of the captured cells in a 24 mm x 4 mm x 50 microm microfluidic chip was obtained by the lensless imaging platform. ...The integrated system gives a CD4 count from blood within 10 min. The integrated platform points a promising direction for …
Gray scale image of the captured cells in a 24 mm x 4 mm x 50 microm microfluidic chip was obtained by the lensless imaging platform. …
Lensless on-chip imaging of cells provides a new tool for high-throughput cell-biology and medical diagnostics.
Mudanyali O, Erlinger A, Seo S, Su TW, Tseng D, Ozcan A. Mudanyali O, et al. Among authors: ozcan a. J Vis Exp. 2009 Dec 14;(34):1650. doi: 10.3791/1650. J Vis Exp. 2009. PMID: 20010542 Free PMC article.
In this report, we introduce experimental details of a lensless on-chip imaging concept termed LUCAS (Lensless Ultra-wide field-of-view Cell monitoring Array platform based on Shadow imaging) that does not require any microscope objectives or other bulky optical components …
In this report, we introduce experimental details of a lensless on-chip imaging concept termed LUCAS (Lensless Ultra-wide field-of-vi …
Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function.
Stybayeva G, Mudanyali O, Seo S, Silangcruz J, Macal M, Ramanculov E, Dandekar S, Erlinger A, Ozcan A, Revzin A. Stybayeva G, et al. Among authors: ozcan a. Anal Chem. 2010 May 1;82(9):3736-44. doi: 10.1021/ac100142a. Anal Chem. 2010. PMID: 20359168 Free PMC article.
Integration of Ab microarrays into a microfluidic flow chamber (4 muL volume) followed by incubation with human blood resulted in capture of CD4 and CD8 T-cells on specific Ab spots. ...The binding of IL-2, TNF-alpha, and IFN-gamma molecules on their respective Ab spots wa …
Integration of Ab microarrays into a microfluidic flow chamber (4 muL volume) followed by incubation with human blood resulted in cap …
Wide field-of-view lens-free fluorescent imaging on a chip.
Coskun AF, Su TW, Ozcan A. Coskun AF, et al. Among authors: ozcan a. Lab Chip. 2010 Apr 7;10(7):824-7. doi: 10.1039/b926561a. Epub 2010 Feb 25. Lab Chip. 2010. PMID: 20379564 Free PMC article.
Such a wide field-of-view lensless fluorescent imaging modality, despite its limited resolution, might be very important for high-throughput screening applications as well as for detection and counting of rare cells within large-area microfluidic devices....
Such a wide field-of-view lensless fluorescent imaging modality, despite its limited resolution, might be very important for high-thr …
On-chip differential interference contrast microscopy using lensless digital holography.
Oh C, Isikman SO, Khademhosseinieh B, Ozcan A. Oh C, et al. Among authors: ozcan a. Opt Express. 2010 Mar 1;18(5):4717-26. doi: 10.1364/OE.18.004717. Opt Express. 2010. PMID: 20389485 Free PMC article.
Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxial crystal and an absorbing polarizer, encoding differential interference contrast information of the objects on the chip. Despite the fact …
Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxi …
Color and monochrome lensless on-chip imaging of Caenorhabditis elegans over a wide field-of-view.
Isikman SO, Sencan I, Mudanyali O, Bishara W, Oztoprak C, Ozcan A. Isikman SO, et al. Among authors: ozcan a. Lab Chip. 2010 May 7;10(9):1109-12. doi: 10.1039/c001200a. Epub 2010 Mar 19. Lab Chip. 2010. PMID: 20390127 Free PMC article.
We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lensless in-line holography. Digital reconstruction of the recorded lensless holograms rapidly creates the C. elegans images within <1 s over …
We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lens …
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