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RNA sequencing: advances, challenges and opportunities.
Ozsolak F, Milos PM. Ozsolak F, et al. Nat Rev Genet. 2011 Feb;12(2):87-98. doi: 10.1038/nrg2934. Epub 2010 Dec 30. Nat Rev Genet. 2011. PMID: 21191423 Free PMC article. Review.
Digital transcriptome profiling from attomole-level RNA samples.
Ozsolak F, Goren A, Gymrek M, Guttman M, Regev A, Bernstein BE, Milos PM. Ozsolak F, et al. Genome Res. 2010 Apr;20(4):519-25. doi: 10.1101/gr.102129.109. Epub 2010 Feb 4. Genome Res. 2010. PMID: 20133332 Free PMC article.
Profiling of short RNAs using Helicos single-molecule sequencing.
Kapranov P, Ozsolak F, Milos PM. Kapranov P, et al. Methods Mol Biol. 2012;822:219-32. doi: 10.1007/978-1-61779-427-8_15. Methods Mol Biol. 2012. PMID: 22144202 Free PMC article.
Single-molecule direct RNA sequencing without cDNA synthesis.
Ozsolak F, Milos PM. Ozsolak F, et al. Wiley Interdiscip Rev RNA. 2011 Jul-Aug;2(4):565-70. doi: 10.1002/wrna.84. Epub 2011 Mar 14. Wiley Interdiscip Rev RNA. 2011. PMID: 21957044 Free PMC article. Review.
Transcriptome profiling using single-molecule direct RNA sequencing.
Ozsolak F, Milos PM. Ozsolak F, et al. Methods Mol Biol. 2011;733:51-61. doi: 10.1007/978-1-61779-089-8_4. Methods Mol Biol. 2011. PMID: 21431762 Free PMC article.
New class of gene-termini-associated human RNAs suggests a novel RNA copying mechanism.
Kapranov P, Ozsolak F, Kim SW, Foissac S, Lipson D, Hart C, Roels S, Borel C, Antonarakis SE, Monaghan AP, John B, Milos PM. Kapranov P, et al. Nature. 2010 Jul 29;466(7306):642-6. doi: 10.1038/nature09190. Nature. 2010. PMID: 20671709 Free PMC article.
Amplification-free digital gene expression profiling from minute cell quantities.
Ozsolak F, Ting DT, Wittner BS, Brannigan BW, Paul S, Bardeesy N, Ramaswamy S, Milos PM, Haber DA. Ozsolak F, et al. Nat Methods. 2010 Aug;7(8):619-21. doi: 10.1038/nmeth.1480. Epub 2010 Jul 18. Nat Methods. 2010. PMID: 20639869 Free PMC article.
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