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1964 4
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1972 1
1974 3
1975 4
1976 8
1977 6
1978 13
1979 12
1980 9
1981 15
1982 21
1983 24
1984 37
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1986 64
1987 92
1988 165
1989 412
1990 1003
1991 2035
1992 3160
1993 4312
1994 5686
1995 6912
1996 8107
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1998 9875
1999 10333
2000 11423
2001 12103
2002 13169
2003 15048
2004 16963
2005 19590
2006 21372
2007 21992
2008 23266
2009 24425
2010 26379
2011 28511
2012 30029
2013 31245
2014 31801
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2016 30460
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675,322 results

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Page 1
Real time quantitative PCR.
Heid CA, Stevens J, Livak KJ, Williams PM. Heid CA, et al. Genome Res. 1996 Oct;6(10):986-94. doi: 10.1101/gr.6.10.986. Genome Res. 1996. PMID: 8908518 Free article.
This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in …
This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time …
Nested Polymerase Chain Reaction (PCR).
Green MR, Sambrook J. Green MR, et al. Cold Spring Harb Protoc. 2019 Feb 1;2019(2). doi: 10.1101/pdb.prot095182. Cold Spring Harb Protoc. 2019. PMID: 30710024
Nested PCR usually involves two sequential amplification reactions, each of which uses a different pair of primers. ...Nested PCR is an efficient method to amplify segments of long templates but requires knowledge of the sequence of the target....
Nested PCR usually involves two sequential amplification reactions, each of which uses a different pair of primers. ...Nested PCR …
Reverse-transcription PCR (RT-PCR).
Bachman J. Bachman J. Methods Enzymol. 2013;530:67-74. doi: 10.1016/B978-0-12-420037-1.00002-6. Methods Enzymol. 2013. PMID: 24034314
RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also co …
RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and ove …
General PCR.
Canene-Adams K. Canene-Adams K. Methods Enzymol. 2013;529:291-8. doi: 10.1016/B978-0-12-418687-3.00024-0. Methods Enzymol. 2013. PMID: 24011055
The primary purpose of polymerase chain reaction (PCR) is to rapidly make many copies of a specific region of DNA or RNA so that it can be adequately detected, often by agarose gel electrophoresis. PCR is routinely used to amplify, modify, and clone genes for expres …
The primary purpose of polymerase chain reaction (PCR) is to rapidly make many copies of a specific region of DNA or RNA so that it c …
Polymerase Chain Reaction (PCR).
Khehra N, Padda IS, Swift CJ. Khehra N, et al. 2023 Mar 6. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan–. 2023 Mar 6. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan–. PMID: 36943981 Free Books & Documents.
In 1985, PCR was introduced by Mullis et al, who were later awarded the Nobel Prize for their work. ...PCR is a procedure that selectively focuses on a minuscule segment of DNA in a test tube. ...
In 1985, PCR was introduced by Mullis et al, who were later awarded the Nobel Prize for their work. ...PCR is a procedure that …
Random mutagenesis by PCR.
Wilson DS, Keefe AD. Wilson DS, et al. Curr Protoc Mol Biol. 2001 May;Chapter 8:Unit8.3. doi: 10.1002/0471142727.mb0803s51. Curr Protoc Mol Biol. 2001. PMID: 18265275
Error-prone PCR (EP-PCR) is the method of choice for introducing random mutations into a defined segment of DNA that is too long to be chemically synthesized as a degenerate sequence. Using EP-PCR, the 5' and 3' boundaries of the mutated region may be defined …
Error-prone PCR (EP-PCR) is the method of choice for introducing random mutations into a defined segment of DNA that is too lo …
Touchdown Polymerase Chain Reaction (PCR).
Green MR, Sambrook J. Green MR, et al. Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095133. Cold Spring Harb Protoc. 2018. PMID: 29717053
"Touchdown polymerase chain reaction (PCR)" is a method to decrease off-target priming and hence to increase the specificity of PCRs. ...To minimize mispriming during the early stages of the PCR, touchdown PCR should always be performed in conjunction with a …
"Touchdown polymerase chain reaction (PCR)" is a method to decrease off-target priming and hence to increase the specificity of PCRs. …
PCR enhancers: Types, mechanisms, and applications in long-range PCR.
Karunanathie H, Kee PS, Ng SF, Kennedy MA, Chua EW. Karunanathie H, et al. Biochimie. 2022 Jun;197:130-143. doi: 10.1016/j.biochi.2022.02.009. Epub 2022 Feb 26. Biochimie. 2022. PMID: 35231536 Review.
They are used to either improve PCR sensitivity, efficiency, and specificity, or mitigate the effects of PCR inhibitors. In this review, we categorise known PCR additives into four main groups. The first three groups comprise PCR additives with well-de …
They are used to either improve PCR sensitivity, efficiency, and specificity, or mitigate the effects of PCR inhibitors. In th …
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology.
Smith CJ, Osborn AM. Smith CJ, et al. FEMS Microbiol Ecol. 2009 Jan;67(1):6-20. doi: 10.1111/j.1574-6941.2008.00629.x. FEMS Microbiol Ecol. 2009. PMID: 19120456 Free article. Review.
Quantitative PCR (Q-PCR or real-time PCR) approaches are now widely applied in microbial ecology to quantify the abundance and expression of taxonomic and functional gene markers within the environment. Q-PCR-based analyses combine 'traditional' end-po …
Quantitative PCR (Q-PCR or real-time PCR) approaches are now widely applied in microbial ecology to quantify the abunda …
Digital PCR: A brief history.
Morley AA. Morley AA. Biomol Detect Quantif. 2014 Aug 15;1(1):1-2. doi: 10.1016/j.bdq.2014.06.001. eCollection 2014 Sep. Biomol Detect Quantif. 2014. PMID: 27920991 Free PMC article. Review.
Digital PCR for quantification of a target of interest has been independently developed several times, being described in 1990 and 1991 using the term "limiting dilution PCR" and in 1999 using the term "digital PCR". It came into use in the decade following i …
Digital PCR for quantification of a target of interest has been independently developed several times, being described in 1990 and 19 …
675,322 results
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