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2006 2
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Establishment of a feeder and serum-free culture system for human embryonic stem cells.
Wang L, Zhang R, Ma R, Jia G, Jian S, Zeng X, Xiong Z, Li B, Li C, Lv Z, Bai X. Wang L, et al. Zygote. 2020 Jun;28(3):175-182. doi: 10.1017/S0967199419000625. Epub 2020 Jan 22. Zygote. 2020. PMID: 31965957
The aim of this study was to develop a feeder-free, serum-free culture method for human embryonic stem cells (hESCs), to establish optimal conditions for hESC proliferation, and to determine the biological characteristics of the resulting hESCs. ...The expres …
The aim of this study was to develop a feeder-free, serum-free culture method for human embryonic stem cells (hESCs), t …
Understanding cell culture dynamics: a tool for defining protocol parameters for improved processes and efficient manufacturing using human embryonic stem cells.
Kusena JWT, Shariatzadeh M, Thomas RJ, Wilson SL. Kusena JWT, et al. Bioengineered. 2021 Dec;12(1):979-996. doi: 10.1080/21655979.2021.1902696. Bioengineered. 2021. PMID: 33757391 Free PMC article.
Standardization is crucial when culturing cells including human embryonic stem cells (hESCs) which are valuable for therapy development and disease modeling. ...Examination of cell dynamics and CPPs permitted the formation of a defined protocol for cul …
Standardization is crucial when culturing cells including human embryonic stem cells (hESCs) which are valuable …
Establishment of drug screening in human embryonic stem cells based on a high-content screening system.
Hua Y, Wu Y, Chun X, Huang H, Yan J, Hu B, Zhang M, Jin L. Hua Y, et al. J Pharmacol Toxicol Methods. 2020 Nov-Dec;106:106913. doi: 10.1016/j.vascn.2020.106913. Epub 2020 Aug 19. J Pharmacol Toxicol Methods. 2020. PMID: 32822830
High-content screening (HCS) systems can be used for high-throughput screening of drugs in human embryonic stem cells (hESCs). However, hESCs require immunofluorescence staining with stemness markers (e.g., Oct-4) prior to HCS, which can be time consuming and …
High-content screening (HCS) systems can be used for high-throughput screening of drugs in human embryonic stem cells ( …
Hematopoietic and endothelial differentiation of human induced pluripotent stem cells.
Choi KD, Yu J, Smuga-Otto K, Salvagiotto G, Rehrauer W, Vodyanik M, Thomson J, Slukvin I. Choi KD, et al. Stem Cells. 2009 Mar;27(3):559-67. doi: 10.1634/stemcells.2008-0922. Stem Cells. 2009. PMID: 19259936 Free PMC article.
Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well as for developing novel approaches for regenerative therapy based on immunologically compatible cells. In this study, we employed an O …
Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well a …
Continuous hypoxic culturing of human embryonic stem cells enhances SSEA-3 and MYC levels.
Närvä E, Pursiheimo JP, Laiho A, Rahkonen N, Emani MR, Viitala M, Laurila K, Sahla R, Lund R, Lähdesmäki H, Jaakkola P, Lahesmaa R. Närvä E, et al. PLoS One. 2013 Nov 13;8(11):e78847. doi: 10.1371/journal.pone.0078847. eCollection 2013. PLoS One. 2013. PMID: 24236059 Free PMC article.
Low oxygen tension (hypoxia) contributes critically to pluripotency of human embryonic stem cells (hESCs) by preventing spontaneous differentiation and supporting self-renewal. ...In this study we characterized the transcriptional and molecular responses of t …
Low oxygen tension (hypoxia) contributes critically to pluripotency of human embryonic stem cells (hESCs) by preventing …
Granulosa cells and retinoic acid co-treatment enrich potential germ cells from manually selected Oct4-EGFP expressing human embryonic stem cells.
Chen HF, Jan PS, Kuo HC, Wu FC, Lan CW, Huang MC, Chien CL, Ho HN. Chen HF, et al. Reprod Biomed Online. 2014 Sep;29(3):319-32. doi: 10.1016/j.rbmo.2014.05.009. Epub 2014 Jun 12. Reprod Biomed Online. 2014. PMID: 25047539
Differentiation of human embryonic stem (HES) cells to germ cells may become clinically useful in overcoming diseases related to germ-cell development. ...H9 Oct4-EGFP[+] cells developed to ovarian follicle-like structures after culture f …
Differentiation of human embryonic stem (HES) cells to germ cells may become clinically useful in overcoming dis …
Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro.
Ovchinnikov DA, Titmarsh DM, Fortuna PR, Hidalgo A, Alharbi S, Whitworth DJ, Cooper-White JJ, Wolvetang EJ. Ovchinnikov DA, et al. Stem Cell Res. 2014 Sep;13(2):251-61. doi: 10.1016/j.scr.2014.05.006. Epub 2014 Jun 8. Stem Cell Res. 2014. PMID: 25108530 Free article.
Here we report on the generation and characterisation of clonal human embryonic stem (HES3, H9) and human induced pluripotent stem cell lines (UQEW01i-epifibC11) that have been stably modified with an artificial EOS(C3+) promoter driving expression of …
Here we report on the generation and characterisation of clonal human embryonic stem (HES3, H9) and human induced pluri …
Cryopreservation by slow cooling with DMSO diminished production of Oct-4 pluripotency marker in human embryonic stem cells.
Katkov II, Kim MS, Bajpai R, Altman YS, Mercola M, Loring JF, Terskikh AV, Snyder EY, Levine F. Katkov II, et al. Cryobiology. 2006 Oct;53(2):194-205. doi: 10.1016/j.cryobiol.2006.05.005. Epub 2006 Jul 12. Cryobiology. 2006. PMID: 16839540
We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of plu …
We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line …
C-MYC controlled TIP110 protein expression regulates OCT4 mRNA splicing in human embryonic stem cells.
Liu Y, Timani K, Ou X, Broxmeyer HE, He JJ. Liu Y, et al. Stem Cells Dev. 2013 Mar 1;22(5):689-94. doi: 10.1089/scd.2012.0271. Epub 2012 Dec 12. Stem Cells Dev. 2013. PMID: 23088399 Free PMC article.
We reported previously that HIV-1 the Tat-interacting protein of 110 kDa (TIP110; P110(NRB)/SART3/p110) is important in regulation of hematopoiesis, and in maintaining pluripotent factor (NANOG, OCT4, and SOX2) expression in and pluripotency of human embryonic stem
We reported previously that HIV-1 the Tat-interacting protein of 110 kDa (TIP110; P110(NRB)/SART3/p110) is important in regulation of hemato …
An immortalized drug-resistant cell line established from 12-13-day mouse embryos for the propagation of human embryonic stem cells.
Iuchi S, Marsch-Moreno M, Velez-DelValle C, Easley K, Kuri-Harcuch W, Green H. Iuchi S, et al. Differentiation. 2006 Apr;74(4):160-6. doi: 10.1111/j.1432-0436.2006.00067.x. Differentiation. 2006. PMID: 16683986
Human embryonic stem (ES) cells are usually co-cultivated with supporting cells consisting of short-term cultures of fibroblasts (not an immortalized line) in a medium lacking serum. ...It supports the growth of H9 cells as well as do ava …
Human embryonic stem (ES) cells are usually co-cultivated with supporting cells consisting of short-term culture …
16 results