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Methenyltetrahydrofolate cyclohydrolase is rate limiting for the enzymatic conversion of 10-formyltetrahydrofolate to 5,10-methylenetetrahydrofolate in bifunctional dehydrogenase-cyclohydrolase enzymes.
Pawelek PD, MacKenzie RE. Pawelek PD, et al. Biochemistry. 1998 Jan 27;37(4):1109-15. doi: 10.1021/bi971906t. Biochemistry. 1998. PMID: 9454603
Kinetic analyses reveal multiple steps in forming TonB-FhuA complexes from Escherichia coli.
Khursigara CM, De Crescenzo G, Pawelek PD, Coulton JW. Khursigara CM, et al. Among authors: pawelek pd. Biochemistry. 2005 Mar 8;44(9):3441-53. doi: 10.1021/bi047882p. Biochemistry. 2005. PMID: 15736954
Enzymatic adenylation of 2,3-dihydroxybenzoate is enhanced by a protein-protein interaction between Escherichia coli 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EntA) and 2,3-dihydroxybenzoate-AMP ligase (EntE).
Khalil S, Pawelek PD. Khalil S, et al. Among authors: pawelek pd. Biochemistry. 2011 Feb 1;50(4):533-45. doi: 10.1021/bi101558v. Epub 2010 Dec 31. Biochemistry. 2011. PMID: 21166461
A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds.
Lei Y, Pawelek PD, Powlowski J. Lei Y, et al. Among authors: pawelek pd. Biochemistry. 2008 Jul 1;47(26):6870-82. doi: 10.1021/bi800349k. Epub 2008 Jun 7. Biochemistry. 2008. PMID: 18537268
Methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Photobacterium phosphoreum shares properties with a mammalian mitochondrial homologue.
Pawelek PD, MacKenzie RE. Pawelek PD, et al. Biochim Biophys Acta. 1996 Aug 15;1296(1):47-54. doi: 10.1016/0167-4838(96)00052-0. Biochim Biophys Acta. 1996. PMID: 8765228
Channeling efficiency in the bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase domain: the effects of site-directed mutagenesis of NADP binding residues.
Pawelek PD, Allaire M, Cygler M, MacKenzie RE. Pawelek PD, et al. Biochim Biophys Acta. 2000 Jun 15;1479(1-2):59-68. doi: 10.1016/s0167-4838(00)00058-3. Biochim Biophys Acta. 2000. PMID: 11004530
Identification of the gene responsible for methylmalonic aciduria and homocystinuria, cblC type.
Lerner-Ellis JP, Tirone JC, Pawelek PD, Doré C, Atkinson JL, Watkins D, Morel CF, Fujiwara TM, Moras E, Hosack AR, Dunbar GV, Antonicka H, Forgetta V, Dobson CM, Leclerc D, Gravel RA, Shoubridge EA, Coulton JW, Lepage P, Rommens JM, Morgan K, Rosenblatt DS. Lerner-Ellis JP, et al. Among authors: pawelek pd. Nat Genet. 2006 Jan;38(1):93-100. doi: 10.1038/ng1683. Epub 2005 Nov 27. Nat Genet. 2006. PMID: 16311595
Deletion of the proline-rich region of TonB disrupts formation of a 2:1 complex with FhuA, an outer membrane receptor of Escherichia coli.
Khursigara CM, De Crescenzo G, Pawelek PD, Coulton JW. Khursigara CM, et al. Among authors: pawelek pd. Protein Sci. 2005 May;14(5):1266-73. doi: 10.1110/ps.051342505. Epub 2005 Mar 31. Protein Sci. 2005. PMID: 15802653 Free PMC article.
Siderophore transport through Escherichia coli outer membrane receptor FhuA with disulfide-tethered cork and barrel domains.
Eisenhauer HA, Shames S, Pawelek PD, Coulton JW. Eisenhauer HA, et al. Among authors: pawelek pd. J Biol Chem. 2005 Aug 26;280(34):30574-80. doi: 10.1074/jbc.M506708200. Epub 2005 Jun 30. J Biol Chem. 2005. PMID: 15994322
Interactions between TonB from Escherichia coli and the periplasmic protein FhuD.
Carter DM, Miousse IR, Gagnon JN, Martinez E, Clements A, Lee J, Hancock MA, Gagnon H, Pawelek PD, Coulton JW. Carter DM, et al. Among authors: pawelek pd. J Biol Chem. 2006 Nov 17;281(46):35413-24. doi: 10.1074/jbc.M607611200. Epub 2006 Aug 23. J Biol Chem. 2006. PMID: 16928679
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