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Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta(DE3).
Tegel H, Tourle S, Ottosson J, Persson A. Tegel H, et al. Protein Expr Purif. 2010 Feb;69(2):159-67. doi: 10.1016/j.pep.2009.08.017. Epub 2009 Sep 4. Protein Expr Purif. 2010. PMID: 19733669
Initially a test set of 68 recombinant proteins that previously had been expressed in BL21(DE3) was retransformed and expressed in Rosetta(DE3). ...The content of rare codons showed to have a significant effect on the protein purity. Based on the results of this stu …
Initially a test set of 68 recombinant proteins that previously had been expressed in BL21(DE3) was retransformed and expressed in Ro …
A global view of protein expression in human cells, tissues, and organs.
Pontén F, Gry M, Fagerberg L, Lundberg E, Asplund A, Berglund L, Oksvold P, Björling E, Hober S, Kampf C, Navani S, Nilsson P, Ottosson J, Persson A, Wernérus H, Wester K, Uhlén M. Pontén F, et al. Mol Syst Biol. 2009;5:337. doi: 10.1038/msb.2009.93. Epub 2009 Dec 22. Mol Syst Biol. 2009. PMID: 20029370 Free PMC article.
A detailed analysis of over 2 million manually annotated, high-resolution, immunohistochemistry-based images showed a high fraction (>65%) of expressed proteins in most cells and tissues, with very few proteins (<2%) detected in any single cell type. ...
A detailed analysis of over 2 million manually annotated, high-resolution, immunohistochemistry-based images showed a high fra
Antibody performance in western blot applications is context-dependent.
Algenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S. Algenäs C, et al. Biotechnol J. 2014 Mar;9(3):435-45. doi: 10.1002/biot.201300341. Epub 2014 Jan 29. Biotechnol J. 2014. PMID: 24403002
A comparative study of WB and IHC showed that the performance of antibodies is application-specific, although a correlation between no WB staining and weak IHC staining could be seen. ...Then, more than 82% of the antibodies yielded a specific band correspond
A comparative study of WB and IHC showed that the performance of antibodies is application-specific, although a correlation be
A genecentric Human Protein Atlas for expression profiles based on antibodies.
Berglund L, Björling E, Oksvold P, Fagerberg L, Asplund A, Szigyarto CA, Persson A, Ottosson J, Wernérus H, Nilsson P, Lundberg E, Sivertsson A, Navani S, Wester K, Kampf C, Hober S, Pontén F, Uhlén M. Berglund L, et al. Mol Cell Proteomics. 2008 Oct;7(10):2019-27. doi: 10.1074/mcp.R800013-MCP200. Mol Cell Proteomics. 2008. PMID: 18669619 Review.
An attractive path forward in proteomics is to experimentally annotate the human protein complement of the genome in a genecentric manner. ...The exact antigen sequence for the internally generated antibodies has also been released together with a visualization of t …
An attractive path forward in proteomics is to experimentally annotate the human protein complement of the genome in a genecentric ma …
A human protein atlas based on antibody proteomics.
Persson A, Hober S, Uhlén M. Persson A, et al. Curr Opin Mol Ther. 2006 Jun;8(3):185-90. Curr Opin Mol Ther. 2006. PMID: 16774037 Review.
The Human Protein Atlas is a comprehensive database that provides the protein expression profiles for a large number of human proteins, presented as immunohistological images from most human tissues. ...
The Human Protein Atlas is a comprehensive database that provides the protein expression profiles for a large number of human …
A human protein atlas for normal and cancer tissues based on antibody proteomics.
Uhlén M, Björling E, Agaton C, Szigyarto CA, Amini B, Andersen E, Andersson AC, Angelidou P, Asplund A, Asplund C, Berglund L, Bergström K, Brumer H, Cerjan D, Ekström M, Elobeid A, Eriksson C, Fagerberg L, Falk R, Fall J, Forsberg M, Björklund MG, Gumbel K, Halimi A, Hallin I, Hamsten C, Hansson M, Hedhammar M, Hercules G, Kampf C, Larsson K, Lindskog M, Lodewyckx W, Lund J, Lundeberg J, Magnusson K, Malm E, Nilsson P, Odling J, Oksvold P, Olsson I, Oster E, Ottosson J, Paavilainen L, Persson A, Rimini R, Rockberg J, Runeson M, Sivertsson A, Sköllermo A, Steen J, Stenvall M, Sterky F, Strömberg S, Sundberg M, Tegel H, Tourle S, Wahlund E, Waldén A, Wan J, Wernérus H, Westberg J, Wester K, Wrethagen U, Xu LL, Hober S, Pontén F. Uhlén M, et al. Mol Cell Proteomics. 2005 Dec;4(12):1920-32. doi: 10.1074/mcp.M500279-MCP200. Epub 2005 Aug 27. Mol Cell Proteomics. 2005. PMID: 16127175
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. ...Each image has been annotated by a certified pathologist to provide a knowledge …
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic genera …
Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling.
Nilsson P, Paavilainen L, Larsson K, Odling J, Sundberg M, Andersson AC, Kampf C, Persson A, Al-Khalili Szigyarto C, Ottosson J, Björling E, Hober S, Wernérus H, Wester K, Pontén F, Uhlen M. Nilsson P, et al. Proteomics. 2005 Nov;5(17):4327-37. doi: 10.1002/pmic.200500072. Proteomics. 2005. PMID: 16237735
A great need exists for the systematic generation of specific antibodies to explore the human proteome. Here, we show that antibodies specific to human proteins can be generated in a high-throughput manner involving stringent affinity purification using recombinant
A great need exists for the systematic generation of specific antibodies to explore the human proteome. Here, we show that antibodies
A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum.
Gantelius J, Hamsten C, Neiman M, Schwenk JM, Persson A, Andersson-Svahn H. Gantelius J, et al. J Microbiol Methods. 2010 Jul;82(1):11-8. doi: 10.1016/j.mimet.2010.03.007. Epub 2010 Mar 25. J Microbiol Methods. 2010. PMID: 20346992
Novel analytical methods for a next generation of diagnostic devices combine attributes from sensitive, accurate, fast, simple and multiplexed analysis methods. Here, we describe a possible contribution to these by the application of a lateral flow microarray …
Novel analytical methods for a next generation of diagnostic devices combine attributes from sensitive, accurate, fast, simple and mu …
Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony type.
Hamsten C, Neiman M, Schwenk JM, Hamsten M, March JB, Persson A. Hamsten C, et al. Mol Cell Proteomics. 2009 Nov;8(11):2544-54. doi: 10.1074/mcp.M900009-MCP200. Epub 2009 Aug 20. Mol Cell Proteomics. 2009. PMID: 19696080 Free PMC article.
The potential of the assay to monitor IgG, IgM, and IgA responses over time was shown in a proof-of-concept study with 116 sera from eight animals in a CBPP vaccine study. In conclusion, a toolbox with recombinant proteins and a flexible suspension arr …
The potential of the assay to monitor IgG, IgM, and IgA responses over time was shown in a proof-of-concept study with 116 sera from …
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