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Receptor tyrosine phosphatase beta (RPTPbeta) activity and signaling are attenuated by glycosylation and subsequent cell surface galectin-1 binding.
Abbott KL, Matthews RT, Pierce M. Abbott KL, et al. J Biol Chem. 2008 Nov 28;283(48):33026-35. doi: 10.1074/jbc.M803646200. Epub 2008 Oct 6. J Biol Chem. 2008. PMID: 18838383 Free PMC article.
Comparison of the substrate specificities and catalytic properties of the sister N-acetylglucosaminyltransferases, GnT-V and GnT-Vb (IX).
Alvarez-Manilla G, Troupe K, Fleming M, Martinez-Uribe E, Pierce M. Alvarez-Manilla G, et al. Glycobiology. 2010 Feb;20(2):166-74. doi: 10.1093/glycob/cwp158. Epub 2009 Oct 21. Glycobiology. 2010. PMID: 19846580 Free PMC article.
Moreover, although both enzymes utilized an N-linked asialo-agalacto-biantennary glycan as an acceptor, GnT-Vb displayed an almost 2.5-fold higher apparent K(m) value compared to GnT-V. Conversely, GnT-Vb very efficiently glycosylated a synthetic glycopeptide, Ac-H(2)N-Val …
Moreover, although both enzymes utilized an N-linked asialo-agalacto-biantennary glycan as an acceptor, GnT-Vb displayed an almost 2.5-fold …
Tools for glycomics: relative quantitation of glycans by isotopic permethylation using 13CH3I.
Alvarez-Manilla G, Warren NL, Abney T, Atwood J 3rd, Azadi P, York WS, Pierce M, Orlando R. Alvarez-Manilla G, et al. Glycobiology. 2007 Jul;17(7):677-87. doi: 10.1093/glycob/cwm033. Epub 2007 Mar 23. Glycobiology. 2007. PMID: 17384119
Deletion of mouse embryo fibroblast N-acetylglucosaminyltransferase V stimulates alpha5beta1 integrin expression mediated by the protein kinase C signaling pathway.
Guo HB, Lee I, Bryan BT, Pierce M. Guo HB, et al. J Biol Chem. 2005 Mar 4;280(9):8332-42. doi: 10.1074/jbc.M413532200. Epub 2004 Dec 22. J Biol Chem. 2005. PMID: 15615721
Analysis of the site-specific N-glycosylation of beta1,6 N-acetylglucosaminyltransferase V.
Kamar M, Alvarez-Manilla G, Abney T, Azadi P, Kumar Kolli VS, Orlando R, Pierce M. Kamar M, et al. Glycobiology. 2004 Jul;14(7):583-92. doi: 10.1093/glycob/cwh062. Epub 2004 Apr 14. Glycobiology. 2004. PMID: 15084511
Transcriptional regulation of the protocadherin β cluster during Her-2 protein-induced mammary tumorigenesis results from altered N-glycan branching.
Guo H, Nairn A, dela Rosa M, Nagy T, Zhao S, Moremen K, Pierce M. Guo H, et al. J Biol Chem. 2012 Jul 20;287(30):24941-54. doi: 10.1074/jbc.M112.369355. Epub 2012 Jun 4. J Biol Chem. 2012. PMID: 22665489 Free PMC article.
Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis.
Abbott KL, Lim JM, Wells L, Benigno BB, McDonald JF, Pierce M. Abbott KL, et al. Proteomics. 2010 Feb;10(3):470-81. doi: 10.1002/pmic.200900537. Proteomics. 2010. PMID: 19953551 Free PMC article.
Regulation of homotypic cell-cell adhesion by branched N-glycosylation of N-cadherin extracellular EC2 and EC3 domains.
Guo HB, Johnson H, Randolph M, Pierce M. Guo HB, et al. J Biol Chem. 2009 Dec 11;284(50):34986-97. doi: 10.1074/jbc.M109.060806. Epub 2009 Oct 21. J Biol Chem. 2009. PMID: 19846557 Free PMC article.
., Kamar, M., and Pierce, M. (2003) J. Biol. Chem. 278, 52412-52424). A detailed study using site-directed mutagenesis demonstrated that three of the eight putative N-glycosylation sites in the N-cadherin sequence showed N-glycan expression. ...
., Kamar, M., and Pierce, M. (2003) J. Biol. Chem. 278, 52412-52424). A detailed study using site-directed mutagenesis …
Glycoproteomic analysis of embryonic stem cells: identification of potential glycobiomarkers using lectin affinity chromatography of glycopeptides.
Alvarez-Manilla G, Warren NL, Atwood J 3rd, Orlando R, Dalton S, Pierce M. Alvarez-Manilla G, et al. J Proteome Res. 2010 May 7;9(5):2062-75. doi: 10.1021/pr8007489. J Proteome Res. 2010. PMID: 19545112 Free PMC article.
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