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Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli.
Pack P, Plückthun A. Pack P, et al. Biochemistry. 1992 Feb 18;31(6):1579-84. doi: 10.1021/bi00121a001. Biochemistry. 1992. PMID: 1737014
They are based on single-chain FV fragments, with a flexible hinge region from mouse IgG3 and an amphiphilic helix fused to the C-terminus of the antibody fragment. The sequence of the helix was taken either from that of a previously reported four-helix bundle desig …
They are based on single-chain FV fragments, with a flexible hinge region from mouse IgG3 and an amphiphilic helix fused to the C-ter …
Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy.
Morfill J, Blank K, Zahnd C, Luginbühl B, Kühner F, Gottschalk KE, Plückthun A, Gaub HE. Morfill J, et al. Biophys J. 2007 Nov 15;93(10):3583-90. doi: 10.1529/biophysj.107.112532. Epub 2007 Aug 3. Biophys J. 2007. PMID: 17675348 Free PMC article.
Using single-molecule force spectroscopy, we have characterized three variants of a recombinant antibody single-chain Fv fragment. ...Therefore, they are closely related and differ from each other by a few mutations only. The dissociation rates determined with the a …
Using single-molecule force spectroscopy, we have characterized three variants of a recombinant antibody single-chain Fv fragment. .. …
Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. I. GroEL recognizes the signal sequences of beta-lactamase precursor.
Zahn R, Axmann SE, Rücknagel KP, Jaeger E, Laminet AA, Plückthun A. Zahn R, et al. J Mol Biol. 1994 Sep 16;242(2):150-64. doi: 10.1006/jmbi.1994.1566. J Mol Biol. 1994. PMID: 7916381
The thermodynamic intermediate H of Escherichia coli beta-lactamase and its precursor had no enzymatic activity, and a quenched tryptophan fluorescence intensity, but a native-like wavelength of maximum intensity. ...We suggest a thermodynamic partitioning mo …
The thermodynamic intermediate H of Escherichia coli beta-lactamase and its precursor had no enzymatic activity, and a quenched trypt …
The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines.
Bothmann H, Pluckthun A. Bothmann H, et al. J Biol Chem. 2000 Jun 2;275(22):17100-5. doi: 10.1074/jbc.M910233199. J Biol Chem. 2000. PMID: 10748200
We used a selection system for periplasmic chaperones based on the coexpression of an E. coli library with a poorly expressing antibody single-chain Fv (scFv) fragment displayed on filamentous phage (Bothmann, H., and Plückthun, A. (1998) Nature Biotechnol. 1 …
We used a selection system for periplasmic chaperones based on the coexpression of an E. coli library with a poorly expressing …
Structural changes of the Escherichia coli GroEL-GroES chaperonins upon complex formation in solution: a neutron small angle scattering study.
Stegmann R, Manakova E, Rössle M, Heumann H, Nieba-Axmann SE, Plückthun A, Hermann T, May RP, Wiedenmann A. Stegmann R, et al. J Struct Biol. 1998 Jan;121(1):30-40. doi: 10.1006/jsbi.1997.3938. J Struct Biol. 1998. PMID: 9573618
Each subunit of the heptameric GroES consists of two domains, a core domain (Met1 to Lys15 and Lys34 to Ala97) and an intervening loop region (Glu16 to Ala33). ...Upon complex formation the apical domains of GroEL move outwards, which facilitates binding of GroES at a
Each subunit of the heptameric GroES consists of two domains, a core domain (Met1 to Lys15 and Lys34 to Ala97) and an intervening loo …
Fast selection of antibodies without antigen purification: adaptation of the protein fragment complementation assay to select antigen-antibody pairs.
Mössner E, Koch H, Plückthun A. Mössner E, et al. J Mol Biol. 2001 Apr 27;308(2):115-22. doi: 10.1006/jmbi.2001.4575. J Mol Biol. 2001. PMID: 11327756
We believe that this system will be very powerful as a routine system for generating antibodies, especially in functional genomics, since it does not require purification and immobilization of the antigen. The identification of an antibody specific for a cDNA or EST …
We believe that this system will be very powerful as a routine system for generating antibodies, especially in functional genomics, s …
Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display.
Hanes J, Schaffitzel C, Knappik A, Plückthun A. Hanes J, et al. Nat Biotechnol. 2000 Dec;18(12):1287-92. doi: 10.1038/82407. Nat Biotechnol. 2000. PMID: 11101809
Here we applied ribosome display to in vitro selection and evolution of single-chain antibody fragments (scFvs) from a large synthetic library (Human Combinatorial Antibody Library; HuCAL) against bovine insulin. ...We have thus mimicked the process of antibody generation …
Here we applied ribosome display to in vitro selection and evolution of single-chain antibody fragments (scFvs) from a large syntheti …
Model and simulation of multivalent binding to fixed ligands.
Müller KM, Arndt KM, Plückthun A. Müller KM, et al. Anal Biochem. 1998 Aug 1;261(2):149-58. doi: 10.1006/abio.1998.2725. Anal Biochem. 1998. PMID: 9716417
The binding of a bivalent molecule (the analyte) to the ligand is described as occurring in two steps, the second of which is driven by the local concentration of neighboring ligands. ...The simulation quantitates the influence of bivalent binding on the sensor signal, as …
The binding of a bivalent molecule (the analyte) to the ligand is described as occurring in two steps, the second of which is driven …
Antigen binding forces of individually addressed single-chain Fv antibody molecules.
Ros R, Schwesinger F, Anselmetti D, Kubon M, Schäfer R, Plückthun A, Tiefenauer L. Ros R, et al. Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7402-5. doi: 10.1073/pnas.95.13.7402. Proc Natl Acad Sci U S A. 1998. PMID: 9636161 Free PMC article.
Antibody single-chain Fv fragment (scFv) molecules that are specific for fluorescein have been engineered with a C-terminal cysteine for a directed immobilization on a flat gold surface. ...For selected molecules the antigen binding forces are then determined …
Antibody single-chain Fv fragment (scFv) molecules that are specific for fluorescein have been engineered with a C-terminal cysteine …
Competition BIAcore for measuring true affinities: large differences from values determined from binding kinetics.
Nieba L, Krebber A, Plückthun A. Nieba L, et al. Anal Biochem. 1996 Feb 15;234(2):155-65. doi: 10.1006/abio.1996.0067. Anal Biochem. 1996. PMID: 8714593
Even at the lowest coupling densities useful for measurements, rebinding and bivalency effects offset the measurements by a factor of up to 500. ...
Even at the lowest coupling densities useful for measurements, rebinding and bivalency effects offset the measurements by a factor of …
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