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Characterization of nra, a global negative regulator gene in group A streptococci.
Podbielski A, Woischnik M, Leonard BA, Schmidt KH. Podbielski A, et al. Mol Microbiol. 1999 Feb;31(4):1051-64. doi: 10.1046/j.1365-2958.1999.01241.x. Mol Microbiol. 1999. PMID: 10096074
During sequencing of an 11.5 kb genomic region of a serotype M49 group A streptococcal (GAS) strain, a series of genes were identified including nra(negative regulator of GAS). ...Nra-regulated genes include the fibronectin-binding protein F2 gene (prtF2) and …
During sequencing of an 11.5 kb genomic region of a serotype M49 group A streptococcal (GAS) strain, a series of genes …
Similarity between the group B and A streptococcal C5a peptidase genes.
Cleary PP, Handley J, Suvorov AN, Podbielski A, Ferrieri P. Cleary PP, et al. Infect Immun. 1992 Oct;60(10):4239-44. doi: 10.1128/IAI.60.10.4239-4244.1992. Infect Immun. 1992. PMID: 1398935 Free PMC article.
A 50-bp deletion was located near the 5' end of scpB in a region where repeat sequences are found in scpA12. Hybridization experiments confirmed that other serotypes of GBS also carry an scpB-like gene. These results show that GBS contain a gene, scpB, which
A 50-bp deletion was located near the 5' end of scpB in a region where repeat sequences are found in scpA12. Hybridization exp
M protein gene typing of Streptococcus pyogenes by nonradioactively labeled oligonucleotide probes.
Kaufhold A, Podbielski A, Johnson DR, Kaplan EL, Lütticken R. Kaufhold A, et al. J Clin Microbiol. 1992 Sep;30(9):2391-7. doi: 10.1128/JCM.30.9.2391-2397.1992. J Clin Microbiol. 1992. PMID: 1401004 Free PMC article.
A new approach for the typing of Streptococcus pyogenes is described. Oligonucleotide probes of 30 nucleotides in length were derived from currently known sequences of the N-terminal regions of M protein genes (emm genes). The oligonucleotides were labeled with digoxigenin
A new approach for the typing of Streptococcus pyogenes is described. Oligonucleotide probes of 30 nucleotides in length were derived
Surface protein-CAT reporter fusions demonstrate differential gene expression in the vir regulon of Streptococcus pyogenes.
Podbielski A, Peterson JA, Cleary P. Podbielski A, et al. Mol Microbiol. 1992 Aug;6(16):2253-65. doi: 10.1111/j.1365-2958.1992.tb01401.x. Mol Microbiol. 1992. PMID: 1406266
To facilitate studies of this regulatory unit, we constructed a new shuttle vector with a staphylococcal chloramphenicol acetyl transferase (CAT) reporter box which replicates in S. pyogenes. ...We demonstrated that VirR is a necessary but not always sufficie …
To facilitate studies of this regulatory unit, we constructed a new shuttle vector with a staphylococcal chloramphenicol acety …
The group A streptococcal M-type 3 protein gene exhibits a C terminus typical for class I M proteins.
Podbielski A, Baird R, Kaufhold A. Podbielski A, et al. Med Microbiol Immunol. 1992;181(4):209-13. doi: 10.1007/BF00215766. Med Microbiol Immunol. 1992. PMID: 1435517
The M protein gene (emm gene) from a reference group A streptococcal strain of serotype M3 was amplified by the polymerase chain reaction and partially sequenced. ...Recently published sequence data that were assigned to emm 3 resulted from a strain confusion …
The M protein gene (emm gene) from a reference group A streptococcal strain of serotype M3 was amplified by the polymerase cha …
Ubiquitous occurrence of virR and scpA genes in group A streptococci.
Podbielski A. Podbielski A. Med Microbiol Immunol. 1992;181(4):227-40. doi: 10.1007/BF00215768. Med Microbiol Immunol. 1992. PMID: 1435518
Until now a few serotypes of M-class I group A streptococci (GAS) have been shown to encode VirR, a positive regulatory factor for the coordinate expression of the M protein (emm) and C5a peptidase (scpA) genes. ...Among the serotypes encoding a large- …
Until now a few serotypes of M-class I group A streptococci (GAS) have been shown to encode VirR, a positive regulatory …
Molecular cloning and nucleotide sequence of the group B streptococcal hemolysin.
Conrads G, Podbielski A, Lütticken R. Conrads G, et al. Zentralbl Bakteriol. 1991 Jun;275(2):179-84. doi: 10.1016/s0934-8840(11)80064-2. Zentralbl Bakteriol. 1991. PMID: 1657015
Genomic DNA from a Streptococcus agalactiae strain and pUC8 were used to clone the genetic determinant of the group B streptococcal hemolysin. ...The deduced amino acid sequence of 230 residues shows a hydrophobic signal sequence of 19 amino acids at its N-terminus. …
Genomic DNA from a Streptococcus agalactiae strain and pUC8 were used to clone the genetic determinant of the group B streptococcal h …
Molecular cloning and nucleotide sequence of a new plasmid-coded Klebsiella pneumoniae beta-lactamase gene (SHV-2a) responsible for high-level cefotaxime resistance.
Podbielski A, Schönling J, Melzer B, Warnatz K. Podbielski A, et al. Zentralbl Bakteriol. 1991 Aug;275(3):369-73. doi: 10.1016/s0934-8840(11)80302-6. Zentralbl Bakteriol. 1991. PMID: 1741919
Patient specimen isolates of Klebsiella pneumoniae exhibiting an MIC of greater than 128 mg/l for cefotaxime were shown to produce a beta-lactamase with a pI of 7.6, which is encoded on a 66 kb conjugative plasmid. A 3.5 kb Bam HI fragment of this plas …
Patient specimen isolates of Klebsiella pneumoniae exhibiting an MIC of greater than 128 mg/l for cefotaxime were shown to produce a
Application of the polymerase chain reaction to study the M protein(-like) gene family in beta-hemolytic streptococci.
Podbielski A, Melzer B, Lütticken R. Podbielski A, et al. Med Microbiol Immunol. 1991;180(4):213-27. doi: 10.1007/BF00215250. Med Microbiol Immunol. 1991. PMID: 1784271
Using the "allM" primer pair for PCR on the genomic DNA from GAS of 29 different M types as well as from a group C and a group G streptococcal isolate, DNA fragments within the expected size range were amplified in every assay. ...These data indicate the existence o …
Using the "allM" primer pair for PCR on the genomic DNA from GAS of 29 different M types as well as from a group C and a group …
Different promoters of SHV-2 and SHV-2a beta-lactamase lead to diverse levels of cefotaxime resistance in their bacterial producers.
Podbielski A, Schönling J, Melzer B, Haase G. Podbielski A, et al. J Gen Microbiol. 1991 Jul;137(7):1667-75. doi: 10.1099/00221287-137-7-1667. J Gen Microbiol. 1991. PMID: 1955859
Hybridization of intragenically annealing oligonucleotides to dot-blotted serial dilutions of total cellular RNA from E. coli transformants harbouring these genes cloned into the same vector plasmid gave a positive signal down to 1.2 micrograms (SHV-2) and 0.32 to 0.16 mic …
Hybridization of intragenically annealing oligonucleotides to dot-blotted serial dilutions of total cellular RNA from E. coli transformants …
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