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Molecular characterization of group A streptococcal (GAS) oligopeptide permease (opp) and its effect on cysteine protease production.
Podbielski A, Pohl B, Woischnik M, Körner C, Schmidt KH, Rozdzinski E, Leonard BA. Podbielski A, et al. Mol Microbiol. 1996 Sep;21(5):1087-99. doi: 10.1046/j.1365-2958.1996.661421.x. Mol Microbiol. 1996. PMID: 8885277
A lambda library of the strain CS101 group A streptococcal (GAS) genome was used to sequence 10,192 bp containing the five genes oppA to oppF of the GAS opp operon. ...While growth kinetics, binding of serum proteins, and attachment to eukaryotic cells were unaffect
A lambda library of the strain CS101 group A streptococcal (GAS) genome was used to sequence 10,192 bp containing the five gen
Molecular characterization of the cfb gene encoding group B streptococcal CAMP-factor.
Podbielski A, Blankenstein O, Lütticken R. Podbielski A, et al. Med Microbiol Immunol. 1994 Nov;183(5):239-56. doi: 10.1007/BF00198458. Med Microbiol Immunol. 1994. PMID: 7715536
Within the 1560 bp sequenced, a complete cfb gene deviating in two deduced aa residues from the published sequence was identified. In addition, the cfbR268 sequence contained a 29-aa leader peptide. Using primers directed to the 5' and 3' ends of cfb for PCR, a
Within the 1560 bp sequenced, a complete cfb gene deviating in two deduced aa residues from the published sequence was identified. In …
The group A streptococcal M-type 3 protein gene exhibits a C terminus typical for class I M proteins.
Podbielski A, Baird R, Kaufhold A. Podbielski A, et al. Med Microbiol Immunol. 1992;181(4):209-13. doi: 10.1007/BF00215766. Med Microbiol Immunol. 1992. PMID: 1435517
The M protein gene (emm gene) from a reference group A streptococcal strain of serotype M3 was amplified by the polymerase chain reaction and partially sequenced. ...Recently published sequence data that were assigned to emm 3 resulted from a strain confusion …
The M protein gene (emm gene) from a reference group A streptococcal strain of serotype M3 was amplified by the polymerase cha …
The group A streptococcal virR49 gene controls expression of four structural vir regulon genes.
Podbielski A, Flosdorff A, Weber-Heynemann J. Podbielski A, et al. Infect Immun. 1995 Jan;63(1):9-20. doi: 10.1128/IAI.63.1.9-20.1995. Infect Immun. 1995. PMID: 7806389 Free PMC article.
Within a genomic locus termed the vir regulon, virR genes of opacity factor-nonproducing (OF-) group A streptococci (GAS) are known to control the expression of the genes encoding M protein (emm) and C5a peptidase (scpA) and of virR itself. ...Subsequently, the virR …
Within a genomic locus termed the vir regulon, virR genes of opacity factor-nonproducing (OF-) group A streptococci (GAS) are …
An improved broth-microdilution procedure for testing metal ion resistance of Klebsiella pneumoniae.
Podbielski A, Haase G, Berndt R. Podbielski A, et al. Zentralbl Hyg Umweltmed. 1989 Nov;189(2):147-50. Zentralbl Hyg Umweltmed. 1989. PMID: 2690846
A new broth-microdilution method for testing metal ion resistance was designed in order to circumvent the formation of insoluble complexes between cations and compounds of the test medium. ...
A new broth-microdilution method for testing metal ion resistance was designed in order to circumvent the formation of insoluble comp
Molecular characterization of a new plasmid-encoded SHV-type beta-lactamase (SHV-2 variant) conferring high-level cefotaxime resistance upon Klebsiella pneumoniae.
Podbielski A, Schönling J, Melzer B, Warnatz K, Leusch HG. Podbielski A, et al. J Gen Microbiol. 1991 Mar;137(3):569-78. doi: 10.1099/00221287-137-3-569. J Gen Microbiol. 1991. PMID: 2033379
Isoelectric focusing of protein preparations from the transconjugants showed a beta-lactamase with a pI of 7.6. A 3.6 kb BamHI fragment containing the beta-lactamase gene was cloned into pLG339 resulting in the recombinant plasmid pZMP1-1. A restrictio …
Isoelectric focusing of protein preparations from the transconjugants showed a beta-lactamase with a pI of 7.6. A 3.6 k …
Different promoters of SHV-2 and SHV-2a beta-lactamase lead to diverse levels of cefotaxime resistance in their bacterial producers.
Podbielski A, Schönling J, Melzer B, Haase G. Podbielski A, et al. J Gen Microbiol. 1991 Jul;137(7):1667-75. doi: 10.1099/00221287-137-7-1667. J Gen Microbiol. 1991. PMID: 1955859
Hybridization of intragenically annealing oligonucleotides to dot-blotted serial dilutions of total cellular RNA from E. coli transformants harbouring these genes cloned into the same vector plasmid gave a positive signal down to 1.2 micrograms (SHV-2) and 0.32 to 0.16 mic …
Hybridization of intragenically annealing oligonucleotides to dot-blotted serial dilutions of total cellular RNA from E. coli transformants …
Molecular cloning and nucleotide sequence of a new plasmid-coded Klebsiella pneumoniae beta-lactamase gene (SHV-2a) responsible for high-level cefotaxime resistance.
Podbielski A, Schönling J, Melzer B, Warnatz K. Podbielski A, et al. Zentralbl Bakteriol. 1991 Aug;275(3):369-73. doi: 10.1016/s0934-8840(11)80302-6. Zentralbl Bakteriol. 1991. PMID: 1741919
Patient specimen isolates of Klebsiella pneumoniae exhibiting an MIC of greater than 128 mg/l for cefotaxime were shown to produce a beta-lactamase with a pI of 7.6, which is encoded on a 66 kb conjugative plasmid. A 3.5 kb Bam HI fragment of this plas …
Patient specimen isolates of Klebsiella pneumoniae exhibiting an MIC of greater than 128 mg/l for cefotaxime were shown to produce a
Application of the polymerase chain reaction to study the M protein(-like) gene family in beta-hemolytic streptococci.
Podbielski A, Melzer B, Lütticken R. Podbielski A, et al. Med Microbiol Immunol. 1991;180(4):213-27. doi: 10.1007/BF00215250. Med Microbiol Immunol. 1991. PMID: 1784271
Using the "allM" primer pair for PCR on the genomic DNA from GAS of 29 different M types as well as from a group C and a group G streptococcal isolate, DNA fragments within the expected size range were amplified in every assay. ...These data indicate the existence o …
Using the "allM" primer pair for PCR on the genomic DNA from GAS of 29 different M types as well as from a group C and a group …
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