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Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties.
Hudspeth EM, Wang Q, Seid CA, Hammond M, Wei J, Liu Z, Zhan B, Pollet J, Heffernan MJ, McAtee CP, Engler DA, Matsunami RK, Strych U, Asojo OA, Hotez PJ, Bottazzi ME. Hudspeth EM, et al. Among authors: pollet j. Hum Vaccin Immunother. 2016 Jul 2;12(7):1707-20. doi: 10.1080/21645515.2016.1139254. Epub 2016 Feb 2. Hum Vaccin Immunother. 2016. PMID: 26839079 Free PMC article.
Cysteine mutagenesis improves the production without abrogating antigenicity of a recombinant protein vaccine candidate for human chagas disease.
Seid CA, Jones KM, Pollet J, Keegan B, Hudspeth E, Hammond M, Wei J, McAtee CP, Versteeg L, Gutierrez A, Liu Z, Zhan B, Respress JL, Strych U, Bottazzi ME, Hotez PJ. Seid CA, et al. Among authors: pollet j. Hum Vaccin Immunother. 2017 Mar 4;13(3):621-633. doi: 10.1080/21645515.2016.1242540. Epub 2016 Oct 13. Hum Vaccin Immunother. 2017. PMID: 27737611 Free PMC article.
Optimization of the Production Process and Characterization of the Yeast-Expressed SARS-CoV Recombinant Receptor-Binding Domain (RBD219-N1), a SARS Vaccine Candidate.
Chen WH, Chag SM, Poongavanam MV, Biter AB, Ewere EA, Rezende W, Seid CA, Hudspeth EM, Pollet J, McAtee CP, Strych U, Bottazzi ME, Hotez PJ. Chen WH, et al. Among authors: pollet j. J Pharm Sci. 2017 Aug;106(8):1961-1970. doi: 10.1016/j.xphs.2017.04.037. Epub 2017 Apr 26. J Pharm Sci. 2017. PMID: 28456726 Free PMC article.
A method to probe protein structure from UV absorbance spectra.
Biter AB, Pollet J, Chen WH, Strych U, Hotez PJ, Bottazzi ME. Biter AB, et al. Among authors: pollet j. Anal Biochem. 2019 Dec 15;587:113450. doi: 10.1016/j.ab.2019.113450. Epub 2019 Sep 21. Anal Biochem. 2019. PMID: 31550438
Yeast-Expressed SARS-CoV Recombinant Receptor-Binding Domain (RBD219-N1) Formulated with Aluminum Hydroxide Induces Protective Immunity and Reduces Immune Enhancement.
Chen WH, Tao X, Agrawal A, Algaissi A, Peng BH, Pollet J, Strych U, Bottazzi ME, Hotez PJ, Lustigman S, Du L, Jiang S, Tseng CK. Chen WH, et al. Among authors: pollet j. bioRxiv [Preprint]. 2020 Jul 5:2020.05.15.098079. doi: 10.1101/2020.05.15.098079. bioRxiv. 2020. PMID: 32511385 Free PMC article. Updated. Preprint.
211 results