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A versatile quick-prep of genomic DNA from gram-positive bacteria.
Pospiech A, Neumann B. Pospiech A, et al. Trends Genet. 1995 Jun;11(6):217-8. doi: 10.1016/s0168-9525(00)89052-6. Trends Genet. 1995. PMID: 7638902 No abstract available.
Rapid isolation of genomic DNA from gram-negative bacteria.
Neumann B, Pospiech A, Schairer HU. Neumann B, et al. Among authors: pospiech a. Trends Genet. 1992 Oct;8(10):332-3. doi: 10.1016/0168-9525(92)90269-a. Trends Genet. 1992. PMID: 1475843 No abstract available.
A new Myxococcus xanthus gene cluster for the biosynthesis of the antibiotic saframycin Mx1 encoding a peptide synthetase.
Pospiech A, Cluzel B, Bietenhader J, Schupp T. Pospiech A, et al. Microbiology (Reading). 1995 Aug;141 ( Pt 8):1793-803. doi: 10.1099/13500872-141-8-1793. Microbiology (Reading). 1995. PMID: 7551044
Gene disruption experiments defined a > or = 18 kb contiguous DNA region involved in saframycin biosynthesis. Sequencing of part of this region revealed a large ORF containing two 600-amino-acid domains with similarity to peptide synthetase amino-acid-activating …
Gene disruption experiments defined a > or = 18 kb contiguous DNA region involved in saframycin biosynthesis. Sequencing of part o …
Two multifunctional peptide synthetases and an O-methyltransferase are involved in the biosynthesis of the DNA-binding antibiotic and antitumour agent saframycin Mx1 from Myxococcus xanthus.
Pospiech A, Bietenhader J, Schupp T. Pospiech A, et al. Microbiology (Reading). 1996 Apr;142 ( Pt 4):741-6. doi: 10.1099/00221287-142-4-741. Microbiology (Reading). 1996. PMID: 8936303
Saframycin Mx1 is a DNA-binding antibiotic and antitumour agent produced by Myxococcus xanthus. It is a heterocyclic quinone, thought to be synthesized via the linear peptide intermediate AlaGlyTyrTyr. ...Complementation tests showed that antibiotic-non-producing mu …
Saframycin Mx1 is a DNA-binding antibiotic and antitumour agent produced by Myxococcus xanthus. It is a heterocyclic quinone, …
A physical and genetic map of the Stigmatella aurantiaca DW4/3.1 chromosome.
Neumann B, Pospiech A, Schairer HU. Neumann B, et al. Among authors: pospiech a. Mol Microbiol. 1993 Dec;10(5):1087-99. doi: 10.1111/j.1365-2958.1993.tb00979.x. Mol Microbiol. 1993. PMID: 7934859
A physical map of the myxobacterium Stigmatella aurantiaca DW4/3.1 chromosome was constructed by pulsed-field gel (PFG) long-range mapping. ...It was thus possible to construct a circular restriction map of the single 9.35 Mbp chromosome of S. aurantiaca based on th
A physical map of the myxobacterium Stigmatella aurantiaca DW4/3.1 chromosome was constructed by pulsed-field gel (PFG) long-range ma
Stigmatella aurantiaca fruiting body formation is dependent on the fbfA gene encoding a polypeptide homologous to chitin synthases.
Silakowski B, Pospiech A, Neumann B, Schairer HU. Silakowski B, et al. Among authors: pospiech a. J Bacteriol. 1996 Dec;178(23):6706-13. doi: 10.1128/jb.178.23.6706-6713.1996. J Bacteriol. 1996. PMID: 8955286 Free PMC article.
Stigmatella aurantiaca is a prokaryotic organism that undergoes a multicellular cycle of development resulting in the formation of a fruiting body. ...About 800 bp upstream of the transposon insertion of mutant AP182 which inactivates a gene (fbfB) inv …
Stigmatella aurantiaca is a prokaryotic organism that undergoes a multicellular cycle of development resulting in the formatio …
Efficient identification of point mutations by automated DNA sequencing of artificial heterozygote samples.
Staedtler F, Pospiech A, Steiner S, Looser M. Staedtler F, et al. Among authors: pospiech a. Mol Biotechnol. 1998 Dec;10(3):269-72. doi: 10.1007/BF02740848. Mol Biotechnol. 1998. PMID: 9951707
By mixing the PCR fragments from two individual mutants in a defined ratio, samples of artificial heterozygous composition were prepared. ...The simultaneous, visual comparison of the mixed mutant traces using a graphics program efficiently revealed all heterozygous …
By mixing the PCR fragments from two individual mutants in a defined ratio, samples of artificial heterozygous composition were prepa …
Substituent effects on the light-induced C-C and C-Br bond activation in (bisphosphine)(eta2-tolane)Pt0 complexes. A TD-DFT study.
Escudero D, Assmann M, Pospiech A, Weigand W, González L. Escudero D, et al. Among authors: pospiech a. Phys Chem Chem Phys. 2009 Jun 14;11(22):4593-600. doi: 10.1039/b903603b. Epub 2009 May 5. Phys Chem Chem Phys. 2009. PMID: 19475180
A theoretical study of the steric and electronic effects of different substituents on the electronic ground and excited states of (bisphosphine)(eta2-tolane)Pt0 complexes is presented. A natural-bond-order (NBO) analysis has been performed to describe the bonding na
A theoretical study of the steric and electronic effects of different substituents on the electronic ground and excited states of (bi
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