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Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system.
Zhang Y, Qin W, Lu X, Xu J, Huang H, Bai H, Li S, Lin S. Zhang Y, et al. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. Nat Commun. 2017. PMID: 28740134 Free PMC article.
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. ...This shows that Cas9 varian …
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base …
Programmable base editing in zebrafish using a modified CRISPR-Cas9 system.
Qin W, Lu X, Lin S. Qin W, et al. Methods. 2018 Nov 1;150:19-23. doi: 10.1016/j.ymeth.2018.07.010. Epub 2018 Aug 2. Methods. 2018. PMID: 30076894
The use of CRISPR/Cas9 to knockout genes in zebrafish has been well established. However, to better model many human diseases that are caused by point mutations, a robust methodology for generating desirable DNA base changes is still needed. ...They ha …
The use of CRISPR/Cas9 to knockout genes in zebrafish has been well established. However, to better model many human di …
CRISPR/Cas9-based genome engineering of zebrafish using a seamless integration strategy.
Luo JJ, Bian WP, Liu Y, Huang HY, Yin Q, Yang XJ, Pei DS. Luo JJ, et al. FASEB J. 2018 Sep;32(9):5132-5142. doi: 10.1096/fj.201800077RR. Epub 2018 May 29. FASEB J. 2018. PMID: 29812974
Numerous feasible methods for inserting large fragments of exogenous DNA sequences into the zebrafish genome have been developed, as has genome editing technology using programmable nucleases. ...-P., Liu, Y., Huang, H.-Y., Yin, Q., Yang, X.-J., …
Numerous feasible methods for inserting large fragments of exogenous DNA sequences into the zebrafish genome have been develop …
A Guide to Computational Tools and Design Strategies for Genome Editing Experiments in Zebrafish Using CRISPR/Cas9.
Prykhozhij SV, Rajan V, Berman JN. Prykhozhij SV, et al. Zebrafish. 2016 Feb;13(1):70-3. doi: 10.1089/zeb.2015.1158. Epub 2015 Dec 18. Zebrafish. 2016. PMID: 26683213
The zebrafish is highly amenable to applications of CRISPR/Cas9 for mutation generation and a variety of DNA insertions. ...Here we provide an in-depth guide on how to use CRISPR/Cas9 and other relevant computational tools at each step of a host …
The zebrafish is highly amenable to applications of CRISPR/Cas9 for mutation generation and a variety of DNA insertions …
Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model.
Yang Z, Chen S, Xue S, Li X, Sun Z, Yang Y, Hu X, Geng T, Cui H. Yang Z, et al. Biotechnol Lett. 2018 Dec;40(11-12):1507-1518. doi: 10.1007/s10529-018-2605-5. Epub 2018 Sep 22. Biotechnol Lett. 2018. PMID: 30244429 Free PMC article.
OBJECTIVES: To investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish. RESULTS: Here we have constructed a transgenic zebrafish strain that can be screened by pigment deficiency. Compared with the traditional CRI …
OBJECTIVES: To investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish. RESULTS: …
foxm1 Modulates Cell Non-Autonomous Response in Zebrafish Skeletal Muscle Homeostasis.
Ferreira FJ, Carvalho L, Logarinho E, Bessa J. Ferreira FJ, et al. Cells. 2021 May 18;10(5):1241. doi: 10.3390/cells10051241. Cells. 2021. PMID: 34070077 Free PMC article.
Recent data have shown that this transcription factor also modulates gene networks associated with other cellular mechanisms, suggesting non-proliferative functions that remain largely unexplored. In this study, we used CRISPR/Cas9 to disrupt foxm1 in the …
Recent data have shown that this transcription factor also modulates gene networks associated with other cellular mechanisms, suggest …
[Complexity of Detecting CRISPR/Cas9-Mediated Homologous Recombination in Zebrafish].
Pi Y, He KZ, Zhang WQ, Dong ZQ, Jiang FG, Jiang KJ, Guo S. Pi Y, et al. Mol Biol (Mosk). 2020 May-Jun;54(3):435-444. doi: 10.31857/S0026898420030131. Mol Biol (Mosk). 2020. PMID: 32492006 Free article. Russian.
Homology-directed (HD) genome modification offers an opportunity to precisely modify the genome. Despite reported successful cases, for many loci, precise genome editing remains challenging and inefficient in vivo. Here we report an effort to pr …
Homology-directed (HD) genome modification offers an opportunity to precisely modify the genome. Despite reported succe …
Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish.
Zhang C, Lu T, Zhang Y, Li J, Tarique I, Wen F, Chen A, Wang J, Zhang Z, Zhang Y, Shi DL, Shao M. Zhang C, et al. Sci Adv. 2021 Aug 6;7(32):eabg4243. doi: 10.1126/sciadv.abg4243. Print 2021 Aug. Sci Adv. 2021. PMID: 34362733 Free PMC article.
Notably, most of these maternal mutants displayed either sgRNA site-spanning genomic deletions or unintended large deletions extending distantly from the sgRNA targets, suggesting a prominent deletion-prone tendency of genome editing in the oocyte. Thus, our …
Notably, most of these maternal mutants displayed either sgRNA site-spanning genomic deletions or unintended large deletions extendin …
MAFB modulates the maturation of lymphatic vascular networks in mice.
Rondon-Galeano M, Skoczylas R, Bower NI, Simons C, Gordon E, Francois M, Koltowska K, Hogan BM. Rondon-Galeano M, et al. Dev Dyn. 2020 Oct;249(10):1201-1216. doi: 10.1002/dvdy.209. Epub 2020 Jul 7. Dev Dyn. 2020. PMID: 32525258 Free article.
RESULTS: We generated a Mafb loss-of-function mouse using CRISPR/Cas9 gene editing. Mafb mutant mice presented with perinatal lethality associated with cyanosis. We identify a role for MAFB in modifying lymphatic network morphogenesis in the dev …
RESULTS: We generated a Mafb loss-of-function mouse using CRISPR/Cas9 gene editing. Mafb mutant mice presented w …
Genomic Knockout of Two Presumed Forelimb Tbx5 Enhancers Reveals They Are Nonessential for Limb Development.
Cunningham TJ, Lancman JJ, Berenguer M, Dong PDS, Duester G. Cunningham TJ, et al. Cell Rep. 2018 Jun 12;23(11):3146-3151. doi: 10.1016/j.celrep.2018.05.052. Cell Rep. 2018. PMID: 29898387 Free PMC article.
We used CRISPR/Cas9 gene editing to knockout the endogenous elements and unexpectedly found that deletion of the intron 2 and downstream elements, either singly or together in double knockouts, resulted in no effect on forelimb development. Our finding …
We used CRISPR/Cas9 gene editing to knockout the endogenous elements and unexpectedly found that deletion of the …
11 results