The metabolism of lysophosphatidic acids by allelic variants of human soluble epoxide hydrolase

Endang R Purba; Elsa A Leuhery; Ami Oguro; Susumu Imaoka

doi:10.1016/j.dmpk.2014.09.002

The metabolism of lysophosphatidic acids by allelic variants of human soluble epoxide hydrolase

Drug Metab Pharmacokinet. 2015 Feb;30(1):75-81. doi: 10.1016/j.dmpk.2014.09.002. Epub 2014 Oct 11.

Authors

Endang R Purba¹, Elsa A Leuhery¹, Ami Oguro¹, Susumu Imaoka²

Affiliations

¹ Research Center for Environmental Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Japan; Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Japan.
² Research Center for Environmental Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Japan; Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Japan. Electronic address: imaoka@kwansei.ac.jp.

PMID: 25760533
DOI: 10.1016/j.dmpk.2014.09.002

Abstract

Lysophosphatidic acids (LPAs) are phospholipids which have many physiological and pathophysiological functions. The human soluble epoxide hydrolase (sEH) plays a role in the metabolism of xenobiotics through its metabolism of aromatic hydrocarbon epoxides such as styrene oxide. sEH also has a phosphatase activity, and metabolizes LPAs. In this study, we investigated a purified wild-type (WT) and six allelic variants of sEH to evaluate differences in their activities toward LPAs. We found that the R103C and R287Q showed significantly lower activity than the WT sEH. We also found that the R103C and R287Q had significantly lower activity even when applied to only the N-terminal or C-terminal domain. The kinetic study determined that the R103C and R287Q had a lower Vmax/Km ratio toward stearoyl-LPA than the other variants. In a previous study, we found that WT sEH suppressed vascular endothelial growth factor (VEGF) mRNA in Hep3B cells; in the present experiments, all sEH variants except V442A suppressed VEGF mRNA levels in Hep3B cells. These results suggest that the R103C and R287Q have lower phosphatase activity, but that all the allelic variants have similar effects on VEGF suppression.

Keywords: Allelic variants; Epoxide hydrolase activity; Lysophosphatidic acid; Phosphatase activity; Soluble epoxide hydrolase; Vascular endothelial growth factor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
Cell Culture Techniques
Cell Line, Tumor
Epoxide Hydrolases / genetics*
Epoxide Hydrolases / metabolism*
Escherichia coli / genetics
Humans
Lysophospholipids / metabolism*
Polymorphism, Genetic
Transfection
Vascular Endothelial Growth Factor A / antagonists & inhibitors
Xenobiotics / metabolism*

Substances

Lysophospholipids
VEGFA protein, human
Vascular Endothelial Growth Factor A
Xenobiotics
Epoxide Hydrolases