Oncolytic vaccinia virus in combination with radiation shows synergistic antitumor efficacy in pancreatic cancer

M H Dai; S L Liu; N G Chen; T P Zhang; L You; F Q Zhang; T C Chou; A A Szalay; Y Fong; Y P Zhao

doi:10.1016/j.canlet.2013.11.007

Oncolytic vaccinia virus in combination with radiation shows synergistic antitumor efficacy in pancreatic cancer

Cancer Lett. 2014 Mar 28;344(2):282-90. doi: 10.1016/j.canlet.2013.11.007. Epub 2013 Dec 7.

Authors

M H Dai¹, S L Liu¹, N G Chen², T P Zhang¹, L You¹, F Q Zhang³, T C Chou⁴, A A Szalay⁵, Y Fong⁶, Y P Zhao⁷

Affiliations

¹ Department of Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
² Genelux Corporation, San Diego Science Center, San Diego, CA 92109, USA; Department of Radiation Medicine and Applied Sciences, Rebecca & John Moores Comprehensive Cancer Center, University of California, San Diego, CA 92093, USA.
³ Department of Radiotherapy, Peking Union Medical College Hospital, Beijing 100730, China.
⁴ Department of Preclinical Pharmacology, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
⁵ Genelux Corporation, San Diego Science Center, San Diego, CA 92109, USA; Department of Radiation Medicine and Applied Sciences, Rebecca & John Moores Comprehensive Cancer Center, University of California, San Diego, CA 92093, USA; Department of Biochemistry, Rudolf Virchow Center for Experimental Biomedicine, Institute for Molecular Infection Biology, University of Wuerzburg, Wuerzburg, Germany.
⁶ Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA. Electronic address: FongY@mskcc.org.
⁷ Department of Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. Electronic address: Zhaoyp8028@gmail.com.

PMID: 24321381
DOI: 10.1016/j.canlet.2013.11.007

Abstract

Combining oncolytic viruses with conventional therapy such as radiation is an innovative option for pancreatic cancer. We demonstrated that combination of GLV-1h151 and radiation yielded a synergistic cytotoxic effect, with the greatest effect achieved in the AsPC-1cell line. Combination treatment significantly increased apoptosis compared with either single treatment or the control group. In mice bearing human pancreatic tumor xenografts, combination treatment resulted in significantly enhanced inhibition of tumor growth. No evidence of toxicity was observed in mice. These results indicate that the combination of GLV-1h151 and radiation has great potential for translation into clinic practice.

Keywords: Pancreatic cancer; Synergism; Virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / radiotherapy
Adenocarcinoma / therapy*
Adenocarcinoma / virology
Animals
Cell Line, Tumor
Combined Modality Therapy
Humans
Mice
Mice, Inbred BALB C
Oncolytic Virotherapy / methods*
Pancreatic Neoplasms / radiotherapy
Pancreatic Neoplasms / therapy*
Pancreatic Neoplasms / virology
Random Allocation
Vaccinia virus / physiology*
Xenograft Model Antitumor Assays