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Quantification of mutant alleles in circulating tumor DNA can predict survival in lung cancer.
Yang X, Zhuo M, Ye X, Bai H, Wang Z, Sun Y, Zhao J, An T, Duan J, Wu M, Wang J. Yang X, et al. Oncotarget. 2016 Apr 12;7(15):20810-24. doi: 10.18632/oncotarget.8021. Oncotarget. 2016. PMID: 26989078 Free PMC article.
PURPOSE: We aimed to investigate the feasibility of droplet digital PCR (ddPCR) for the quantitative and dynamic detection of EGFR mutations and next generation sequencing (NGS) for screening EGFR-tyrosine kinase inhibitors (EGFR-TKIs) resistance-relevant mutations in circulat ā€¦
PURPOSE: We aimed to investigate the feasibility of droplet digital PCR (ddPCR) for the quantitative and dynamic detection of EGFR mutations ā€¦
High MAF of EGFR mutations and high ratio of T790M sensitizing mutations in ctDNA predict better third-generation TKI outcomes.
Li Y, Zhang F, Yuan P, Guo L, Jianming Y, He J. Li Y, et al. Thorac Cancer. 2020 Jun;11(6):1503-1511. doi: 10.1111/1759-7714.13418. Epub 2020 Apr 14. Thorac Cancer. 2020. PMID: 32285618 Free PMC article.
BACKGROUND: Clinical detection of EGFR-TKI resistance mechanism through tissue can be really challenging due to risks associated with the procedure. Thus, liquid biopsy, especially circulation tumor DNA (ctDNA) analysis, can be an adequate source for biomarker testi ā€¦
BACKGROUND: Clinical detection of EGFR-TKI resistance mechanism through tissue can be really challenging due to risks associated with the pr ā€¦
BRAF mutation analysis in circulating free tumor DNA of melanoma patients treated with BRAF inhibitors.
Gonzalez-Cao M, Mayo-de-Las-Casas C, Molina-Vila MA, De Mattos-Arruda L, MuƱoz-Couselo E, Manzano JL, Cortes J, Berros JP, Drozdowskyj A, Sanmamed M, Gonzalez A, Alvarez C, Viteri S, Karachaliou N, Martin Algarra S, Bertran-Alamillo J, Jordana-Ariza N, Rosell R. Gonzalez-Cao M, et al. Melanoma Res. 2015 Dec;25(6):486-95. doi: 10.1097/CMR.0000000000000187. Melanoma Res. 2015. PMID: 26366702
We developed a rapid, sensitive method for the detection and quantification of BRAFV600E in circulating free DNA (cfDNA) isolated from plasma and serum on the basis of a quantitative 5'-nuclease PCR (Taqman) in the presence of a peptide-nucleic acid. ...This ā€¦
We developed a rapid, sensitive method for the detection and quantification of BRAFV600E in circulating free DNA (cfDNA ā€¦
LungBEAM: A prospective multicenter study to monitor stage IV NSCLC patients with EGFR mutations using BEAMing technology.
Garrido P, Paz-Ares L, Majem M, MorĆ”n T, Trigo JM, Bosch-Barrera J, GarcĪÆa-Campelo R, GonzĆ”lez-Larriba JL, SĆ”nchez-Torres JM, Isla D, ViƱolas N, Camps C, Insa A, Juan Ɠ, Massuti B, Paredes A, Artal Ɓ, LĆ³pez-Brea M, Palacios J, Felip E. Garrido P, et al. Cancer Med. 2021 Sep;10(17):5878-5888. doi: 10.1002/cam4.4135. Epub 2021 Jul 23. Cancer Med. 2021. PMID: 34296539 Free PMC article.
OBJECTIVES: The aim of LungBEAM was to determine the value of a novel epidermal growth factor receptor (EGFR) mutation test in blood based on BEAMing technology to predict disease progression in advanced non-small cell lung cancer (NSCLC) patients treated wit ā€¦
OBJECTIVES: The aim of LungBEAM was to determine the value of a novel epidermal growth factor receptor (EGFR) mutation test in blood based o ā€¦