Abstract
It is generally thought that cell growth and metabolism regulate cell division and not vice versa. Here, we examined Saccharomyces cerevisiae cells growing under conditions of continuous culture in a chemostat. We found that loss of G1 cyclins, or inactivation of the cyclin-dependent kinase Cdc28p, reduced the activity of glutamate synthase (Glt1p), a key enzyme in nitrogen assimilation. We also present evidence indicating that the G1 cyclin-dependent control of Glt1p may involve Jem1p, a DnaJ-type chaperone. Our results suggest that completion of START may be linked to nitrogen metabolism.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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CDC28 Protein Kinase, S cerevisiae / antagonists & inhibitors
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CDC28 Protein Kinase, S cerevisiae / genetics
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CDC28 Protein Kinase, S cerevisiae / metabolism
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Cell Cycle / physiology
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Cyclins / genetics
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Cyclins / metabolism
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DNA, Fungal / genetics
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G1 Phase / physiology
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Genes, Fungal
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Glutamate Synthase / genetics
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Glutamate Synthase / metabolism
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Nitrogen / metabolism*
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
Substances
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CLN1 protein, S cerevisiae
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CLN2 protein, S cerevisiae
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Cyclins
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DNA, Fungal
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Saccharomyces cerevisiae Proteins
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Glutamate Synthase
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CDC28 Protein Kinase, S cerevisiae
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Nitrogen