Abstract
Zebrafish have emerged as a powerful model system to study leukocyte recruitment and inflammation. Here we characterize the morphology and function of inflammatory macrophages in zebrafish larvae. These macrophages can be distinguished from neutrophils by immunolabeling of L-Plastin without MPO co-expression and by an elongated morphology. Live imaging of transgenic zMPO:GFP larvae demonstrate that GFP(lo) macrophages migrate to wounds by extension of thin pseudopods and carry out phagocytosis of tissue debris, and FACS analysis of leukocyte markers indicates expression of CSF1R in these macrophages. These findings identify distinct functional and morphological characteristics of inflammatory macrophages in zebrafish larvae.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Antigens, Differentiation / genetics
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Antigens, Differentiation / immunology
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Antigens, Differentiation / metabolism*
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Cell Movement / immunology
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Cell Separation
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Flow Cytometry
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Inflammation
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Larva / immunology
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Macrophages / immunology
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Macrophages / metabolism*
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Macrophages / pathology
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / metabolism*
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Microfilament Proteins / genetics
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Microfilament Proteins / immunology
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Microfilament Proteins / metabolism*
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Peroxidase / metabolism
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Phagocytosis
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Pseudopodia / immunology
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Receptor, Macrophage Colony-Stimulating Factor / genetics
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Receptor, Macrophage Colony-Stimulating Factor / immunology
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Receptor, Macrophage Colony-Stimulating Factor / metabolism*
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Zebrafish / immunology*
Substances
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Antigens, Differentiation
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Membrane Glycoproteins
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Microfilament Proteins
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plastin
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Peroxidase
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Receptor, Macrophage Colony-Stimulating Factor