Proteomic analysis of pancreatic zymogen granules: identification of new granule proteins

J Proteome Res. 2007 Aug;6(8):2978-92. doi: 10.1021/pr0607029. Epub 2007 Jun 21.

Abstract

The composition of zymogen granules from rat pancreas was determined by LC-MS/MS. Enriched intragranular content, peripheral membrane, and integral membrane protein fractions were analyzed after one-dimensional SDS-PAGE and tryptic digestion of gel slices. A total of 371 proteins was identified with high confidence, including 84 previously identified granule proteins. The 287 remaining proteins included 37 GTP-binding proteins and effectors, 8 tetraspan membrane proteins, and 22 channels and transporters. Seven proteins, pantophysin, cyclic nucleotide phosphodiesterase, carboxypeptidase D, ecto-nucleotide phosphodiesterase 3, aminopeptidase N, ral, and the potassium channel TWIK-2, were confirmed by immunofluorescence microscopy or by immunoblotting to be new zymogen granule membrane proteins.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Chromatography, Liquid / methods
  • Female
  • Male
  • Pancreas / metabolism*
  • Proteome / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Secretory Vesicles / metabolism*
  • Tandem Mass Spectrometry / methods

Substances

  • Proteome