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72,055 results

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Page 1
RNA-Seq Experiment and Data Analysis.
Withanage MHH, Liang H, Zeng E. Withanage MHH, et al. Methods Mol Biol. 2022;2418:405-424. doi: 10.1007/978-1-0716-1920-9_22. Methods Mol Biol. 2022. PMID: 35119677
Not just the messenger RNA (mRNA), but a wide variety of different RNA populations (e.g., total RNA, microRNA, long ncRNA, pre-mRNA) can also be investigated using RNA-Seq. ...In this chapter, we describe a protocol to construct an RNA- …
Not just the messenger RNA (mRNA), but a wide variety of different RNA populations (e.g., total RNA, microRNA, long ncR …
Cross-species RNA-seq for deciphering host-microbe interactions.
Westermann AJ, Vogel J. Westermann AJ, et al. Nat Rev Genet. 2021 Jun;22(6):361-378. doi: 10.1038/s41576-021-00326-y. Epub 2021 Feb 17. Nat Rev Genet. 2021. PMID: 33597744 Review.
This Review summarizes how next-generation RNA sequencing (RNA-seq) has become a primary technology to study host-microbe interactions with high resolution, improving our understanding of the physiological consequences and the mechanisms at play. We illustrat …
This Review summarizes how next-generation RNA sequencing (RNA-seq) has become a primary technology to study host-micro …
Processing and Analysis of RNA-seq Data from Public Resources.
Zoabi Y, Shomron N. Zoabi Y, et al. Methods Mol Biol. 2021;2243:81-94. doi: 10.1007/978-1-0716-1103-6_4. Methods Mol Biol. 2021. PMID: 33606253 Review.
Advances in next generation sequencing (NGS) technologies resulted in a broad array of large-scale gene expression studies and an unprecedented volume of whole messenger RNA (mRNA) sequencing data, or the transcriptome (also known as RNA sequencing, or RNA- …
Advances in next generation sequencing (NGS) technologies resulted in a broad array of large-scale gene expression studies and an unpreceden …
An RNA-Seq Protocol for Differential Expression Analysis.
Owens NDL, De Domenico E, Gilchrist MJ. Owens NDL, et al. Cold Spring Harb Protoc. 2019 Jun 3;2019(6). doi: 10.1101/pdb.prot098368. Cold Spring Harb Protoc. 2019. PMID: 30952685
Here we consider RNA-Seq, used to measure global gene expression through RNA fragmentation, capture, sequencing, and subsequent computational analysis. ...Here we present a standard RNA-Seq protocol for performing two-state differential gene exp …
Here we consider RNA-Seq, used to measure global gene expression through RNA fragmentation, capture, sequencing, and su …
Dual RNA-Seq of Mtb-Infected Macrophages In Vivo Reveals Ontologically Distinct Host-Pathogen Interactions.
Pisu D, Huang L, Grenier JK, Russell DG. Pisu D, et al. Cell Rep. 2020 Jan 14;30(2):335-350.e4. doi: 10.1016/j.celrep.2019.12.033. Cell Rep. 2020. PMID: 31940480 Free PMC article.
In this work, we explore the in vivo molecular dynamics of Mtb infection by performing dual RNA-seq on Mycobacterium tuberculosis-infected, ontogenetically distinct macrophage lineages isolated directly from murine lungs. ...
In this work, we explore the in vivo molecular dynamics of Mtb infection by performing dual RNA-seq on Mycobacterium tuberculo …
Current RNA-seq methodology reporting limits reproducibility.
Simoneau J, Dumontier S, Gosselin R, Scott MS. Simoneau J, et al. Brief Bioinform. 2021 Jan 18;22(1):140-145. doi: 10.1093/bib/bbz124. Brief Bioinform. 2021. PMID: 31813948 Free PMC article. Review.
Ribonucleic acid sequencing (RNA-seq) identifies and quantifies RNA molecules from a biological sample. Transformation from raw sequencing data to meaningful gene or isoform counts requires an in silico bioinformatics pipeline. ...This work illustrates the ne …
Ribonucleic acid sequencing (RNA-seq) identifies and quantifies RNA molecules from a biological sample. Transformation …
Single-cell RNA-Seq reveals a highly coordinated transcriptional program in mouse germ cells during primordial follicle formation.
He Y, Chen Q, Dai J, Cui Y, Zhang C, Wen X, Li J, Xiao Y, Peng X, Liu M, Shen B, Sha J, Hu Z, Li J, Shu W. He Y, et al. Aging Cell. 2021 Jul;20(7):e13424. doi: 10.1111/acel.13424. Epub 2021 Jun 26. Aging Cell. 2021. PMID: 34174788 Free PMC article.
To delineate the developmental trajectory and regulatory mechanisms of oocytes during the process, we performed RNA-seq on single germ cells from newborn (P0.5) ovaries. Three cell clusters were classified which corresponded to three cell states (germ cell cyst, cys …
To delineate the developmental trajectory and regulatory mechanisms of oocytes during the process, we performed RNA-seq on sin …
Improved RNA-seq normalization.
Fletcher M. Fletcher M. Nat Genet. 2022 Nov;54(11):1584. doi: 10.1038/s41588-022-01217-6. Nat Genet. 2022. PMID: 36333507 No abstract available.
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