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94,929 results

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Page 1
RNA-Seq Experiment and Data Analysis.
Withanage MHH, Liang H, Zeng E. Withanage MHH, et al. Methods Mol Biol. 2022;2418:405-424. doi: 10.1007/978-1-0716-1920-9_22. Methods Mol Biol. 2022. PMID: 35119677
Not just the messenger RNA (mRNA), but a wide variety of different RNA populations (e.g., total RNA, microRNA, long ncRNA, pre-mRNA) can also be investigated using RNA-Seq. ...In this chapter, we describe a protocol to construct an RNA- …
Not just the messenger RNA (mRNA), but a wide variety of different RNA populations (e.g., total RNA, microRNA, long ncR …
RNA-Seq Data Analysis.
Li J, Varghese RS, Ressom HW. Li J, et al. Methods Mol Biol. 2024;2822:263-290. doi: 10.1007/978-1-0716-3918-4_18. Methods Mol Biol. 2024. PMID: 38907924 Free PMC article.
RNA-Seq data analysis stands as a vital part of genomics research, turning vast and complex datasets into meaningful biological insights. It is a field marked by rapid evolution and ongoing innovation, necessitating a thorough understanding for anyone seeking to unl …
RNA-Seq data analysis stands as a vital part of genomics research, turning vast and complex datasets into meaningful biologica …
RNA-seq in Skeletal Biology.
Ayturk U. Ayturk U. Curr Osteoporos Rep. 2019 Aug;17(4):178-185. doi: 10.1007/s11914-019-00517-x. Curr Osteoporos Rep. 2019. PMID: 31093870 Review.
RECENT FINDINGS: Next-generation sequencing of mRNA (RNA-seq) methods have been established and routinely used in skeletal biology research. RNA-seq has led to the identification of novel genes and transcription factors involved in skeletal development …
RECENT FINDINGS: Next-generation sequencing of mRNA (RNA-seq) methods have been established and routinely used in skeletal bio …
Dual RNA-Seq of Mtb-Infected Macrophages In Vivo Reveals Ontologically Distinct Host-Pathogen Interactions.
Pisu D, Huang L, Grenier JK, Russell DG. Pisu D, et al. Cell Rep. 2020 Jan 14;30(2):335-350.e4. doi: 10.1016/j.celrep.2019.12.033. Cell Rep. 2020. PMID: 31940480 Free PMC article.
In this work, we explore the in vivo molecular dynamics of Mtb infection by performing dual RNA-seq on Mycobacterium tuberculosis-infected, ontogenetically distinct macrophage lineages isolated directly from murine lungs. ...
In this work, we explore the in vivo molecular dynamics of Mtb infection by performing dual RNA-seq on Mycobacterium tuberculo …
Current RNA-seq methodology reporting limits reproducibility.
Simoneau J, Dumontier S, Gosselin R, Scott MS. Simoneau J, et al. Brief Bioinform. 2021 Jan 18;22(1):140-145. doi: 10.1093/bib/bbz124. Brief Bioinform. 2021. PMID: 31813948 Free PMC article. Review.
Ribonucleic acid sequencing (RNA-seq) identifies and quantifies RNA molecules from a biological sample. Transformation from raw sequencing data to meaningful gene or isoform counts requires an in silico bioinformatics pipeline. ...This work illustrates the ne …
Ribonucleic acid sequencing (RNA-seq) identifies and quantifies RNA molecules from a biological sample. Transformation …
Best practices on the differential expression analysis of multi-species RNA-seq.
Chung M, Bruno VM, Rasko DA, Cuomo CA, Muñoz JF, Livny J, Shetty AC, Mahurkar A, Dunning Hotopp JC. Chung M, et al. Genome Biol. 2021 Apr 29;22(1):121. doi: 10.1186/s13059-021-02337-8. Genome Biol. 2021. PMID: 33926528 Free PMC article. Review.
Advances in transcriptome sequencing allow for simultaneous interrogation of differentially expressed genes from multiple species originating from a single RNA sample, termed dual or multi-species transcriptomics. Compared to single-species differential expression analysis …
Advances in transcriptome sequencing allow for simultaneous interrogation of differentially expressed genes from multiple species originatin …
Short-Read RNA-Seq.
Hu R, Islam MN, Varghese RS, Ressom HW. Hu R, et al. Methods Mol Biol. 2024;2822:245-262. doi: 10.1007/978-1-0716-3918-4_17. Methods Mol Biol. 2024. PMID: 38907923 Free PMC article.
This chapter outlines the methodology for conducting short-read RNA-Seq, starting from RNA enrichment to library preparation and sequencing. Throughout the chapter, practical tips and best practices are provided to guide researchers in order to optimize each …
This chapter outlines the methodology for conducting short-read RNA-Seq, starting from RNA enrichment to library prepar …
ChIP-seq and RNA-seq Reveal the Involvement of Histone Lactylation Modification in Gestational Diabetes Mellitus.
Huang X, Yip K, Nie H, Chen R, Wang X, Wang Y, Lin W, Li R. Huang X, et al. J Proteome Res. 2024 Jun 7;23(6):1937-1947. doi: 10.1021/acs.jproteome.3c00727. Epub 2024 May 22. J Proteome Res. 2024. PMID: 38776154
This study aims to elucidate the histone lactylation modification landscapes of GDM patients and explore lactylation-modification-related genes involved in GDM. We employed a combination of RNA-seq analysis and chromatin immunoprecipitation sequencing (ChIP-seq …
This study aims to elucidate the histone lactylation modification landscapes of GDM patients and explore lactylation-modification-related ge …
Practical Compass of Single-Cell RNA-Seq Analysis.
Okada H, Chung UI, Hojo H. Okada H, et al. Curr Osteoporos Rep. 2024 Oct;22(5):433-440. doi: 10.1007/s11914-023-00840-4. Epub 2023 Nov 29. Curr Osteoporos Rep. 2024. PMID: 38019344 Free PMC article. Review.
RECENT FINDINGS: The number of studies using single-cell RNA-seq (scRNA-seq) is increasing. scRNA-seq revealed the heterogeneity or diversity of the cellular populations. scRNA-seq also provides insight into the interactions between different ce …
RECENT FINDINGS: The number of studies using single-cell RNA-seq (scRNA-seq) is increasing. scRNA-seq revealed t …
Cross-species RNA-seq for deciphering host-microbe interactions.
Westermann AJ, Vogel J. Westermann AJ, et al. Nat Rev Genet. 2021 Jun;22(6):361-378. doi: 10.1038/s41576-021-00326-y. Epub 2021 Feb 17. Nat Rev Genet. 2021. PMID: 33597744 Review.
This Review summarizes how next-generation RNA sequencing (RNA-seq) has become a primary technology to study host-microbe interactions with high resolution, improving our understanding of the physiological consequences and the mechanisms at play. We illustrat …
This Review summarizes how next-generation RNA sequencing (RNA-seq) has become a primary technology to study host-micro …
94,929 results
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