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Guanosine tetraphosphate-induced dissociation of open complexes at the Escherichia coli ribosomal protein promoters rplJ and rpsA P1: nanosecond depolarization spectroscopic studies.
Raghavan A, Chatterji D. Raghavan A, et al. Biophys Chem. 1998 Oct 5;75(1):21-32. doi: 10.1016/s0301-4622(98)00186-0. Biophys Chem. 1998. PMID: 9810686
This is either accompanied by, or leads to the dissociation of a fraction of the holoenzyme-bound sigma 70. At the lacUV5 promoter, only a marginal dissociation of RNAP is observed. ...A fraction of the latter species releases the sigma 70 subunit and is unab …
This is either accompanied by, or leads to the dissociation of a fraction of the holoenzyme-bound sigma 70. At the lacUV5 promoter, o …
The differential effects of guanosine tetraphosphate on open complex formation at the Escherichia coli ribosomal protein promoters rplJ and rpsA P1.
Raghavan A, Kameshwari DB, Chatterji D. Raghavan A, et al. Biophys Chem. 1998 Oct 5;75(1):7-19. doi: 10.1016/s0301-4622(98)00185-9. Biophys Chem. 1998. PMID: 9810685
The addition of ppGpp causes a twofold reduction in k2 (isomerisation constant) rplJ and a threefold decrease in KB (equilibrium constant of RNAP binding) at rpsA P1. There is a significant twofold increase in kd at rplJ, compared with smaller changes at rpsA …
The addition of ppGpp causes a twofold reduction in k2 (isomerisation constant) rplJ and a threefold decrease in KB (equilibri …
Evidence for a ppGpp-binding site on Escherichia coli RNA polymerase: proximity relationship with the rifampicin-binding domain.
Reddy PS, Raghavan A, Chatterji D. Reddy PS, et al. Among authors: raghavan a. Mol Microbiol. 1995 Jan;15(2):255-65. doi: 10.1111/j.1365-2958.1995.tb02240.x. Mol Microbiol. 1995. PMID: 7746147
This is characterized by the production of high levels of a regulatory nucleotide, ppGpp, and concomitant curtailment in rRNA synthesis. ...AmNS-ppGpp responded much like a ppGpp molecule in an in vitro total transcription assay at selective promoters. Fluorescence …
This is characterized by the production of high levels of a regulatory nucleotide, ppGpp, and concomitant curtailment in rRNA synthes …
Interaction of calcineurin with a domain of the transcription factor NFAT1 that controls nuclear import.
Luo C, Shaw KT, Raghavan A, Aramburu J, Garcia-Cozar F, Perrino BA, Hogan PG, Rao A. Luo C, et al. Among authors: raghavan a. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8907-12. doi: 10.1073/pnas.93.17.8907. Proc Natl Acad Sci U S A. 1996. PMID: 8799126 Free PMC article.
Here we identify a regulatory region of NFAT1, N terminal to the DNA-binding domain, that controls nuclear import of NFAT1. The regulatory region of NFAT1 binds directly to calcineurin, is a substrate for calcineurin in vitro, and shows regulated subcellular localiz …
Here we identify a regulatory region of NFAT1, N terminal to the DNA-binding domain, that controls nuclear import of NFAT1. The regul …
Immunosuppressive drugs prevent a rapid dephosphorylation of transcription factor NFAT1 in stimulated immune cells.
Shaw KT, Ho AM, Raghavan A, Kim J, Jain J, Park J, Sharma S, Rao A, Hogan PG. Shaw KT, et al. Among authors: raghavan a. Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11205-9. doi: 10.1073/pnas.92.24.11205. Proc Natl Acad Sci U S A. 1995. PMID: 7479966 Free PMC article.
The immunosuppressive drugs cyclosporin A and FK506 interfere with the inducible transcription of cytokine genes in T cells and in other immune cells, in part by preventing the activation of NF-AT (nuclear factor of activated T cells). ...Cyclosporin A prevents the …
The immunosuppressive drugs cyclosporin A and FK506 interfere with the inducible transcription of cytokine genes in T cells and in ot …
Tandem fluorescence imaging of dynamic S-acylation and protein turnover.
Zhang MM, Tsou LK, Charron G, Raghavan AS, Hang HC. Zhang MM, et al. Among authors: raghavan as. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8627-32. doi: 10.1073/pnas.0912306107. Epub 2010 Apr 26. Proc Natl Acad Sci U S A. 2010. PMID: 20421494 Free PMC article.
Here, we describe a tandem labeling and detection method to simultaneously monitor dynamic S-palmitoylation and protein turnover. ...In addition to dually fatty-acylated proteins, this tandem fluorescence imaging method can be generalized to other S-acylated proteins using …
Here, we describe a tandem labeling and detection method to simultaneously monitor dynamic S-palmitoylation and protein turnover. ... …
Selective inhibition of NFAT activation by a peptide spanning the calcineurin targeting site of NFAT.
Aramburu J, Garcia-Cózar F, Raghavan A, Okamura H, Rao A, Hogan PG. Aramburu J, et al. Among authors: raghavan a. Mol Cell. 1998 Apr;1(5):627-37. doi: 10.1016/s1097-2765(00)80063-5. Mol Cell. 1998. PMID: 9660947
NFAT transcription factors play a key role in the immune response. The activation of NFAT proteins is controlled by calcineurin, the calmodulin-dependent phosphatase that is inhibited by the immunosuppressive drugs cyclosporin A and FK506. Here we identify a
NFAT transcription factors play a key role in the immune response. The activation of NFAT proteins is controlled by calcineurin, the …
Concerted dephosphorylation of the transcription factor NFAT1 induces a conformational switch that regulates transcriptional activity.
Okamura H, Aramburu J, García-Rodríguez C, Viola JP, Raghavan A, Tahiliani M, Zhang X, Qin J, Hogan PG, Rao A. Okamura H, et al. Among authors: raghavan a. Mol Cell. 2000 Sep;6(3):539-50. doi: 10.1016/s1097-2765(00)00053-8. Mol Cell. 2000. PMID: 11030334
Dephosphorylation of all thirteen residues is required to mask a nuclear export signal (NES), cause full exposure of a nuclear localization signal (NLS), and promote transcriptional activity. ...Our data suggest that dephosphorylation promotes NFAT1 activation by in …
Dephosphorylation of all thirteen residues is required to mask a nuclear export signal (NES), cause full exposure of a nuclear …
Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.
Wilson JP, Raghavan AS, Yang YY, Charron G, Hang HC. Wilson JP, et al. Among authors: raghavan as. Mol Cell Proteomics. 2011 Mar;10(3):M110.001198. doi: 10.1074/mcp.M110.001198. Epub 2010 Nov 14. Mol Cell Proteomics. 2011. PMID: 21076176 Free PMC article.
Here we report the proteomic analysis of mammalian proteins targeted by a series of fatty acid chemical reporters ranging from myristic to stearic acid. ...The unbiased proteomic analysis of fatty-acylated proteins using chemical reporters has revealed a greater div …
Here we report the proteomic analysis of mammalian proteins targeted by a series of fatty acid chemical reporters ranging from myrist …
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