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Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose.
Ramakrishnan B, Boeggeman E, Qasba PK. Ramakrishnan B, et al. Biochemistry. 2004 Oct 5;43(39):12513-22. doi: 10.1021/bi049007+. Biochemistry. 2004. PMID: 15449940
Site-specific linking of biomolecules via glycan residues using glycosyltransferases.
Qasba PK, Boeggeman E, Ramakrishnan B. Qasba PK, et al. Among authors: ramakrishnan b. Biotechnol Prog. 2008 May-Jun;24(3):520-6. doi: 10.1021/bp0704034. Epub 2008 Apr 22. Biotechnol Prog. 2008. PMID: 18426242 Free PMC article. Review.
Bioconjugation and detection of lactosamine moiety using alpha1,3-galactosyltransferase mutants that transfer C2-modified galactose with a chemical handle.
Pasek M, Ramakrishnan B, Boeggeman E, Manzoni M, Waybright TJ, Qasba PK. Pasek M, et al. Among authors: ramakrishnan b. Bioconjug Chem. 2009 Mar 18;20(3):608-18. doi: 10.1021/bc800534r. Bioconjug Chem. 2009. PMID: 19245254 Free PMC article.
Direct identification of nonreducing GlcNAc residues on N-glycans of glycoproteins using a novel chemoenzymatic method.
Boeggeman E, Ramakrishnan B, Kilgore C, Khidekel N, Hsieh-Wilson LC, Simpson JT, Qasba PK. Boeggeman E, et al. Among authors: ramakrishnan b. Bioconjug Chem. 2007 May-Jun;18(3):806-14. doi: 10.1021/bc060341n. Epub 2007 Mar 20. Bioconjug Chem. 2007. PMID: 17370997 Free PMC article.
Crystal structures of β-1,4-galactosyltransferase 7 enzyme reveal conformational changes and substrate binding.
Tsutsui Y, Ramakrishnan B, Qasba PK. Tsutsui Y, et al. Among authors: ramakrishnan b. J Biol Chem. 2013 Nov 1;288(44):31963-70. doi: 10.1074/jbc.M113.509984. Epub 2013 Sep 19. J Biol Chem. 2013. PMID: 24052259 Free PMC article.
Binding of N-acetylglucosamine (GlcNAc) β1-6-branched oligosaccharide acceptors to β4-galactosyltransferase I reveals a new ligand binding mode.
Ramakrishnan B, Boeggeman E, Qasba PK. Ramakrishnan B, et al. J Biol Chem. 2012 Aug 17;287(34):28666-74. doi: 10.1074/jbc.M112.373514. Epub 2012 Jun 27. J Biol Chem. 2012. PMID: 22740701 Free PMC article.
Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection.
Boeggeman E, Ramakrishnan B, Pasek M, Manzoni M, Puri A, Loomis KH, Waybright TJ, Qasba PK. Boeggeman E, et al. Among authors: ramakrishnan b. Bioconjug Chem. 2009 Jun;20(6):1228-36. doi: 10.1021/bc900103p. Bioconjug Chem. 2009. PMID: 19425533 Free PMC article.
Mutant glycosyltransferases assist in the development of a targeted drug delivery system and contrast agents for MRI.
Qasba PK, Ramakrishnan B, Boeggeman E. Qasba PK, et al. Among authors: ramakrishnan b. AAPS J. 2006 Mar 24;8(1):E190-5. doi: 10.1208/aapsj080123. AAPS J. 2006. PMID: 16584127 Free PMC article. Review.
Substrate-induced conformational changes in glycosyltransferases.
Qasba PK, Ramakrishnan B, Boeggeman E. Qasba PK, et al. Among authors: ramakrishnan b. Trends Biochem Sci. 2005 Jan;30(1):53-62. doi: 10.1016/j.tibs.2004.11.005. Trends Biochem Sci. 2005. PMID: 15653326 Review.
Mutation of arginine 228 to lysine enhances the glucosyltransferase activity of bovine beta-1,4-galactosyltransferase I.
Ramakrishnan B, Boeggeman E, Qasba PK. Ramakrishnan B, et al. Biochemistry. 2005 Mar 8;44(9):3202-10. doi: 10.1021/bi0479454. Biochemistry. 2005. PMID: 15736931
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