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Expression, purification, and characterization of a human complement component C3 analog that lacks the C-terminal C345c domain.
Ramyar KX, Xu X, White NM, Keightley A, Geisbrecht BV. Ramyar KX, et al. J Immunol Methods. 2019 Oct;473:112633. doi: 10.1016/j.jim.2019.07.005. Epub 2019 Jul 15. J Immunol Methods. 2019. PMID: 31319063 Free PMC article.
Using disruptive insertional mutagenesis to identify the in situ structure-function landscape of the Shigella translocator protein IpaB.
Barta ML, Tachiyama S, Muthuramalingam M, Arizmendi O, Villanueva CE, Ramyar KX, Geisbrecht BV, Lovell S, Battaile KP, Picking WL, Picking WD. Barta ML, et al. Among authors: ramyar kx. Protein Sci. 2018 Aug;27(8):1392-1406. doi: 10.1002/pro.3428. Epub 2018 May 3. Protein Sci. 2018. PMID: 29672980 Free PMC article.
Identification of Surface-Exposed Protein Radicals and A Substrate Oxidation Site in A-Class Dye-Decolorizing Peroxidase from Thermomonospora curvata.
Shrestha R, Chen X, Ramyar KX, Hayati Z, Carlson EA, Bossmann SH, Song L, Geisbrecht BV, Li P. Shrestha R, et al. Among authors: ramyar kx. ACS Catal. 2016 Dec 2;6(12):8036-8047. doi: 10.1021/acscatal.6b01952. Epub 2016 Oct 12. ACS Catal. 2016. PMID: 29308294 Free PMC article.
A structurally dynamic N-terminal region drives function of the staphylococcal peroxidase inhibitor (SPIN).
de Jong NWM, Ploscariu NT, Ramyar KX, Garcia BL, Herrera AI, Prakash O, Katz BB, Leidal KG, Nauseef WM, van Kessel KPM, van Strijp JAG, Geisbrecht BV. de Jong NWM, et al. Among authors: ramyar kx. J Biol Chem. 2018 Feb 16;293(7):2260-2271. doi: 10.1074/jbc.RA117.000134. Epub 2018 Jan 5. J Biol Chem. 2018. PMID: 29306874 Free PMC article.
Immune evasion by a staphylococcal inhibitor of myeloperoxidase.
de Jong NWM, Ramyar KX, Guerra FE, Nijland R, Fevre C, Voyich JM, McCarthy AJ, Garcia BL, van Kessel KPM, van Strijp JAG, Geisbrecht BV, Haas PA. de Jong NWM, et al. Among authors: ramyar kx. Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9439-9444. doi: 10.1073/pnas.1707032114. Epub 2017 Aug 14. Proc Natl Acad Sci U S A. 2017. PMID: 28808028 Free PMC article.
The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein.
Woehl JL, Ramyar KX, Katz BB, Walker JK, Geisbrecht BV. Woehl JL, et al. Among authors: ramyar kx. Protein Sci. 2017 Aug;26(8):1595-1608. doi: 10.1002/pro.3195. Epub 2017 May 31. Protein Sci. 2017. PMID: 28512867 Free PMC article.
NlpC/P60 domain-containing proteins of Mycobacterium avium subspecies paratuberculosis that differentially bind and hydrolyze peptidoglycan.
Bannantine JP, Lingle CK, Adam PR, Ramyar KX, McWhorter WJ, Stabel JR, Picking WD, Geisbrecht BV. Bannantine JP, et al. Among authors: ramyar kx. Protein Sci. 2016 Apr;25(4):840-51. doi: 10.1002/pro.2884. Epub 2016 Feb 14. Protein Sci. 2016. PMID: 26799947 Free PMC article.
Identification of peptidic inhibitors of the alternative complement pathway based on Staphylococcus aureus SCIN proteins.
Summers BJ, Garcia BL, Woehl JL, Ramyar KX, Yao X, Geisbrecht BV. Summers BJ, et al. Among authors: ramyar kx. Mol Immunol. 2015 Oct;67(2 Pt B):193-205. doi: 10.1016/j.molimm.2015.05.012. Epub 2015 Jun 4. Mol Immunol. 2015. PMID: 26052070 Free PMC article.
The extracellular adherence protein from Staphylococcus aureus inhibits the classical and lectin pathways of complement by blocking formation of the C3 proconvertase.
Woehl JL, Stapels DAC, Garcia BL, Ramyar KX, Keightley A, Ruyken M, Syriga M, Sfyroera G, Weber AB, Zolkiewski M, Ricklin D, Lambris JD, Rooijakkers SHM, Geisbrecht BV. Woehl JL, et al. Among authors: ramyar kx. J Immunol. 2014 Dec 15;193(12):6161-6171. doi: 10.4049/jimmunol.1401600. Epub 2014 Nov 7. J Immunol. 2014. PMID: 25381436 Free PMC article.
Staphylococcus aureus secretes a unique class of neutrophil serine protease inhibitors.
Stapels DA, Ramyar KX, Bischoff M, von Köckritz-Blickwede M, Milder FJ, Ruyken M, Eisenbeis J, McWhorter WJ, Herrmann M, van Kessel KP, Geisbrecht BV, Rooijakkers SH. Stapels DA, et al. Among authors: ramyar kx. Proc Natl Acad Sci U S A. 2014 Sep 9;111(36):13187-92. doi: 10.1073/pnas.1407616111. Epub 2014 Aug 26. Proc Natl Acad Sci U S A. 2014. PMID: 25161283 Free PMC article.
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