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2019 1
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Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay.
Zhao F, Hu Y, Fan Z, Huang B, Wei L, Xie Y, Huang Y, Mei S, Wang L, Wang L, Ai B, Fang J, Liang C, Xu F, Tan W, Guo F. Zhao F, et al. Cell Rep Methods. 2023 Oct 23;3(10):100620. doi: 10.1016/j.crmeth.2023.100620. Epub 2023 Oct 16. Cell Rep Methods. 2023. PMID: 37848032 Free PMC article.
Mpox is caused by a zoonotic virus belonging to the Orthopoxvirus genus and the Poxviridae family. In this study, we develop a recombinase polymerase amplification (RPA)-coupled CRISPR-Cas12a detection assay for the mpox virus. ... …
Mpox is caused by a zoonotic virus belonging to the Orthopoxvirus genus and the Poxviridae family. In this study, we develop a rec …
Recombinase polymerase amplification assay for rapid detection of Monkeypox virus.
Davi SD, Kissenkötter J, Faye M, Böhlken-Fascher S, Stahl-Hennig C, Faye O, Faye O, Sall AA, Weidmann M, Ademowo OG, Hufert FT, Czerny CP, Abd El Wahed A. Davi SD, et al. Diagn Microbiol Infect Dis. 2019 Sep;95(1):41-45. doi: 10.1016/j.diagmicrobio.2019.03.015. Epub 2019 Apr 11. Diagn Microbiol Infect Dis. 2019. PMID: 31126795 Free PMC article.
In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed. ...The MPXV-RPA-assa …
In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) usin …
Rapid detection of monkeypox virus using a CRISPR-Cas12a mediated assay: a laboratory validation and evaluation study.
Low SJ, O'Neill MT, Kerry WJ, Krysiak M, Papadakis G, Whitehead LW, Savic I, Prestedge J, Williams L, Cooney JP, Tran T, Lim CK, Caly L, Towns JM, Bradshaw CS, Fairley C, Chow EPF, Chen MY, Pellegrini M, Pasricha S, Williamson DA. Low SJ, et al. Lancet Microbe. 2023 Oct;4(10):e800-e810. doi: 10.1016/S2666-5247(23)00148-9. Epub 2023 Sep 15. Lancet Microbe. 2023. PMID: 37722405 Free article.
BACKGROUND: The 2022 outbreak of mpox (formerly known as monkeypox) led to the spread of monkeypox virus (MPXV) in over 110 countries, demanding effective disease management and surveillance. ...FINDINGS: With an optimal run duration of approximately 45 min f …
BACKGROUND: The 2022 outbreak of mpox (formerly known as monkeypox) led to the spread of monkeypox virus (MPXV) in over …
Rapid detection of mpox virus using recombinase aided amplification assay.
Cui X, Du B, Feng J, Feng Y, Cui J, Yan C, Zhao H, Gan L, Fan Z, Fu T, Xu Z, Zhang R, Du S, Zhou Y, Tian Z, Zhang Q, Fu H, Xue G, Yuan J. Cui X, et al. Front Cell Infect Microbiol. 2023 Feb 23;13:1008783. doi: 10.3389/fcimb.2023.1008783. eCollection 2023. Front Cell Infect Microbiol. 2023. PMID: 36909721 Free PMC article.
In this study, we developed a recombinase-aided amplification (RAA) assay that can detect mpox virus within 5-10 minutes. ...In summary, the RAA mpox assay described here exhibits rapid detection, high sensitivity and specif …
In this study, we developed a recombinase-aided amplification (RAA) assay that can detect mpox virus with …
Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of Monkeypox Virus.
Mao L, Ying J, Selekon B, Gonofio E, Wang X, Nakoune E, Wong G, Berthet N. Mao L, et al. Viruses. 2022 Sep 23;14(10):2112. doi: 10.3390/v14102112. Viruses. 2022. PMID: 36298667 Free PMC article.
Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), in which outbreaks mainly occurred in West and Central Africa, with only sporadic spillovers to countries outside Africa due to international travel or close contact with wildlife. ...Here, thr …
Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), in which outbreaks mainly occurred in West and Centra …
A field diagnostic method for rapid and sensitive detection of mpox virus.
Zhao F, Xu F, Wang X, Song R, Hu Y, Wei L, Xie Y, Huang Y, Mei S, Wang L, Wang L, Gao Z, Guo L, Fang J, Ren L, Jin R, Wang J, Guo F. Zhao F, et al. J Med Virol. 2024 Feb;96(2):e29469. doi: 10.1002/jmv.29469. J Med Virol. 2024. PMID: 38376919
In this study, we have developed an MPXV detection tool that leverages the recombinase-aid amplification assay by integrating lateral flow strips (RAA-LF) and one-step sample DNA preparation, with visible readout, no need of laboratory instrument, and …
In this study, we have developed an MPXV detection tool that leverages the recombinase-aid amplification assay b …
Rapid, sensitive, and highly specific detection of monkeypox virus by CRISPR-based diagnostic platform.
Gong L, Chen X, Wang Y, Liang J, Liu X, Wang Y. Gong L, et al. Front Public Health. 2023 Jun 27;11:1137968. doi: 10.3389/fpubh.2023.1137968. eCollection 2023. Front Public Health. 2023. PMID: 37441636 Free PMC article.
BACKGROUND: Monkeypox (MPX), caused by the Monkeypox virus (MPXV), has incurred global attention since it broke out in many countries in recent times, which highlights the need for rapid and reliable diagnosis of MPXV infection. ...The results of MPXV- …
BACKGROUND: Monkeypox (MPX), caused by the Monkeypox virus (MPXV), has incurred global attention since it broke out in …
CRISPR-Cas12-based field-deployable system for rapid detection of synthetic DNA sequence of the monkeypox virus genome.
Chen Q, Gul I, Liu C, Lei Z, Li X, Raheem MA, He Q, Haihui Z, Leeansyah E, Zhang CY, Pandey V, Du K, Qin P. Chen Q, et al. J Med Virol. 2023 Jan;95(1):e28385. doi: 10.1002/jmv.28385. J Med Virol. 2023. PMID: 36478250
The global outbreak of the monkeypox virus (MPXV) highlights the need for rapid and cost-effective MPXV detection tools to effectively monitor and control the monkeypox disease. Herein, we demonstrated a portable CRISPR-Cas-based system for nake …
The global outbreak of the monkeypox virus (MPXV) highlights the need for rapid and cost-effective MPXV detection …
Development of a CRISPR/Cas12a-recombinase polymerase amplification assay for visual and highly specific identification of the Congo Basin and West African strains of mpox virus.
Yang X, Zeng X, Chen X, Huang J, Wei X, Ying X, Tan Q, Wang Y, Li S. Yang X, et al. J Med Virol. 2023 May;95(5):e28757. doi: 10.1002/jmv.28757. J Med Virol. 2023. PMID: 37212293
In this study, a novel diagnostic protocol utilizing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 12a nuclease (CRISPR/Cas12a)-mediated recombinase polymerase amplification (RPA) was developed to identify mpox in the Co …
In this study, a novel diagnostic protocol utilizing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 12 …
Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device.
Wang Y, Chen H, Lin K, Han Y, Gu Z, Wei H, Mu K, Wang D, Liu L, Jin R, Song R, Rong Z, Wang S. Wang Y, et al. Nat Commun. 2024 Apr 16;15(1):3279. doi: 10.1038/s41467-024-47518-8. Nat Commun. 2024. PMID: 38627378 Free PMC article.
The emerging monkeypox virus (MPXV) has raised global health concern, thereby highlighting the need for rapid, sensitive, and easy-to-use diagnostics. Here, we develop a single-step CRISPR-based diagnostic platform, termed SCOPE (Streamlined CRISPR On Pod Eva …
The emerging monkeypox virus (MPXV) has raised global health concern, thereby highlighting the need for rapid, sensitiv …
14 results