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Reconstituting actomyosin-dependent mechanosensitive protein complexes in vitro.
Ciobanasu C, Faivre B, Le Clainche C. Ciobanasu C, et al. Nat Protoc. 2015 Jan;10(1):75-89. doi: 10.1038/nprot.2014.200. Epub 2014 Dec 11. Nat Protoc. 2015. PMID: 25502885
In many mechanosensitive biological processes, actin-binding proteins (ABPs) sense the force generated by the actomyosin cytoskeleton and respond by recruiting effector proteins. We developed an in vitro assay, with pure proteins, to obse …
In many mechanosensitive biological processes, actin-binding proteins (ABPs) sense the force generated by the actomyosin
Actomyosin-dependent formation of the mechanosensitive talin-vinculin complex reinforces actin anchoring.
Ciobanasu C, Faivre B, Le Clainche C. Ciobanasu C, et al. Nat Commun. 2014;5:3095. doi: 10.1038/ncomms4095. Nat Commun. 2014. PMID: 24452080 Free PMC article.
This process is thought to involve the mechanical unfolding of talin to expose cryptic vinculin-binding sites. However, the ability of the actomyosin cytoskeleton to directly control the formation of a talin-vinculin complex and the resulting activity of the comp
This process is thought to involve the mechanical unfolding of talin to expose cryptic vinculin-binding sites. However, the ability of the …