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Derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate for enhancing the ionization yield of small peptides and glycopeptides in matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry.
Ullmer R, Plematl A, Rizzi A. Ullmer R, et al. Rapid Commun Mass Spectrom. 2006;20(9):1469-79. doi: 10.1002/rcm.2464. Rapid Commun Mass Spectrom. 2006. PMID: 16586471
Glycopeptides with a large carbohydrate part and a short peptide backbone are particularly affected by this problem. To meet the goal of generating mass spectra exhibiting glycopeptide coverages as complete as possible, derivatization of glycopeptides offers a
Glycopeptides with a large carbohydrate part and a short peptide backbone are particularly affected by this problem. To meet t …
Determination of D-alanine and D-glutamic acid in biological samples by coupled-column chromatography using beta-cyclodextrin as mobile phase additive.
Rizzi AM, Briza P, Breitenbach M. Rizzi AM, et al. J Chromatogr. 1992 Nov 6;582(1-2):35-40. doi: 10.1016/0378-4347(92)80299-6. J Chromatogr. 1992. PMID: 1491055
A high-performance liquid chromatographic assay for monitoring traces of D-alanine and D-glutamic acid in peptide and protein hydrolysates is presented, which uses a two-column set-up with column switching. ...A non-chiral alkyl-silica reversed-phase column i
A high-performance liquid chromatographic assay for monitoring traces of D-alanine and D-glutamic acid in peptide and protein hydroly
Matrix-assisted laser desorption/ionization time-of-flight and nano-electrospray ionization ion trap mass spectrometric characterization of 1-cyano-2-substituted-benz[f]isoindole derivatives of peptides for fluorescence detection.
Linnemayr K, Brückner A, Körner R, Hahn R, Jungbauer A, Josic D, Roepstorff P, Rizzi A, Allmaier G. Linnemayr K, et al. J Mass Spectrom. 1999 Apr;34(4):427-34. doi: 10.1002/(SICI)1096-9888(199904)34:4<427::AID-JMS779>3.0.CO;2-C. J Mass Spectrom. 1999. PMID: 10226367
A series of hexa- to decapeptides (molecular mass range 800-1200) were labeled with naphthalene-2,3-dicarboxaldehyde, which preferentially reacts with the primary amino groups of a peptide. ...Furthermore, the fragmentation behavior of the N-terminally tagged peptid
A series of hexa- to decapeptides (molecular mass range 800-1200) were labeled with naphthalene-2,3-dicarboxaldehyde, which preferent
Quantitative isomer-specific N-glycan fingerprinting using isotope coded labeling and high performance liquid chromatography-electrospray ionization-mass spectrometry with graphitic carbon stationary phase.
Michael C, Rizzi AM. Michael C, et al. J Chromatogr A. 2015 Feb 27;1383:88-95. doi: 10.1016/j.chroma.2015.01.028. Epub 2015 Jan 19. J Chromatogr A. 2015. PMID: 25638265
Quantitative profiling of O-glycans by electrospray ionization- and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry after in-gel derivatization with isotope-coded 1-phenyl-3-methyl-5-pyrazolone.
Sić S, Maier NM, Rizzi AM. Sić S, et al. Anal Chim Acta. 2016 Sep 7;935:187-96. doi: 10.1016/j.aca.2016.06.032. Epub 2016 Jun 22. Anal Chim Acta. 2016. PMID: 27543027
For this purpose, a derivatization strategy using d0/d5-1-phenyl-3-methyl-5-pyrazolone (PMP) is employed, allowing O-glycan release and derivatization to be achieved in one single step. ...We also provide a comprehensive overview on the performance of MS-based glyca …
For this purpose, a derivatization strategy using d0/d5-1-phenyl-3-methyl-5-pyrazolone (PMP) is employed, allowing O-glycan release a …
Tandem mass spectrometry of isomeric aniline-labeled N-glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans.
Michael C, Rizzi AM. Michael C, et al. Rapid Commun Mass Spectrom. 2015 Jul 15;29(13):1268-78. doi: 10.1002/rcm.7208. Rapid Commun Mass Spectrom. 2015. PMID: 26395610
The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase. Identification of structural features of separated isobaric isomers was performed by CID-based tandem mass spectrometry (MS/MS) carried ou …
The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase …
Quantitative fingerprinting of O-linked glycans released from proteins using isotopic coded labeling with deuterated 1-phenyl-3-methyl-5-pyrazolone.
Sić S, Maier NM, Rizzi AM. Sić S, et al. J Chromatogr A. 2015 Aug 21;1408:93-100. doi: 10.1016/j.chroma.2015.06.065. Epub 2015 Jul 2. J Chromatogr A. 2015. PMID: 26184710
In this paper, a method is established aimed at improved qualitative and quantitative analysis of mucin-type O-glycans. A chemical reaction combining release and derivatization of O-glycans in one step is combined here with mass spectrometric quantification. ...For …
In this paper, a method is established aimed at improved qualitative and quantitative analysis of mucin-type O-glycans. A chem …
Capillary isoelectric focusing hyphenated to single- and multistage matrix-assisted laser desorption/ionization-mass spectrometry using automated sheath-flow-assisted sample deposition.
Lechner M, Seifner A, Rizzi AM. Lechner M, et al. Electrophoresis. 2008 May;29(10):1974-84. doi: 10.1002/elps.200700836. Electrophoresis. 2008. PMID: 18425755
In this paper CIEF combined with MALDI-MS is described using a sheath-liquid-assisted automatic sample deposition from the separation capillary onto a MALDI target. ...Finally, a test sample of five standard proteins demonstrates the suitability of this techn …
In this paper CIEF combined with MALDI-MS is described using a sheath-liquid-assisted automatic sample deposition from the separation …
Use of a novel ionic liquid matrix for MALDI-MS analysis of glycopeptides and glycans out of total tryptic digests.
Ullmer R, Rizzi AM. Ullmer R, et al. J Mass Spectrom. 2009 Nov;44(11):1596-603. doi: 10.1002/jms.1668. J Mass Spectrom. 2009. PMID: 19787684
In this study, we investigated a novel ionic liquid matrix (ILM), namely, the 1,1,3,3-tetramethylguanidinium salt of 2,4,6-trihydroxyacetophenone (THAP). ...Altogether, GTHAP proved as a useful ILM particularly being superior to solid matrices in the context of glyc …
In this study, we investigated a novel ionic liquid matrix (ILM), namely, the 1,1,3,3-tetramethylguanidinium salt of 2,4,6-trihydroxy …
Relative quantitation of glycosylation variants by stable isotope labeling of enzymatically released N-glycans using [12C]/[13C] aniline and ZIC-HILIC-ESI-TOF-MS.
Giménez E, Sanz-Nebot V, Rizzi A. Giménez E, et al. Anal Bioanal Chem. 2013 Sep;405(23):7307-19. doi: 10.1007/s00216-013-7178-5. Epub 2013 Jul 12. Anal Bioanal Chem. 2013. PMID: 23846592
In a second step, zwitterionic-hydrophilic interaction liquid chromatography in capillary columns coupled to electrospray mass spectrometry with time-of-flight analyzer (μZIC-HILIC-ESI-TOF-MS) was applied for the analysis of labeled N-glycans released from intact glycoprot …
In a second step, zwitterionic-hydrophilic interaction liquid chromatography in capillary columns coupled to electrospray mass spectr …
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