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A C. elegans mutant that lives twice as long as wild type.
Kenyon C, Chang J, Gensch E, Rudner A, Tabtiang R. Kenyon C, et al. Among authors: rudner a. Nature. 1993 Dec 2;366(6454):461-4. doi: 10.1038/366461a0. Nature. 1993. PMID: 8247153
This lifespan extension, the largest yet reported in any organism, requires the activity of a second gene, daf-16. Both genes also regulate formation of the dauer larva, a developmentally arrested larval form that is induced by crowding and starvation and is very lo …
This lifespan extension, the largest yet reported in any organism, requires the activity of a second gene, daf-16. Both genes also re …
Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast.
Minshull J, Straight A, Rudner AD, Dernburg AF, Belmont A, Murray AW. Minshull J, et al. Among authors: rudner ad. Curr Biol. 1996 Dec 1;6(12):1609-20. doi: 10.1016/s0960-9822(02)70784-7. Curr Biol. 1996. PMID: 8994825
In contrast, a newly isolated mutant in the budding yeast CDC55 gene, which encodes a protein phosphatase 2A (PP2A) regulatory subunit, shows a different checkpoint defect. In the presence of a defective spindle, these cells separate their sister chrom …
In contrast, a newly isolated mutant in the budding yeast CDC55 gene, which encodes a protein phosphatase 2A (PP2A) regulatory …
Cdc28 activates exit from mitosis in budding yeast.
Rudner AD, Hardwick KG, Murray AW. Rudner AD, et al. J Cell Biol. 2000 Jun 26;149(7):1361-76. doi: 10.1083/jcb.149.7.1361. J Cell Biol. 2000. PMID: 10871278 Free PMC article.
CDC28-T18V, Y19F (CDC28-VF), a mutant that lacks inhibitory phosphorylation sites, delays the exit from mitosis and is hypersensitive to perturbations that arrest cells in mitosis. Surprisingly, this behavior is not due to a lack of inhibitory phosphorylation or inc …
CDC28-T18V, Y19F (CDC28-VF), a mutant that lacks inhibitory phosphorylation sites, delays the exit from mitosis and is hypersensitive …
Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae.
Hardwick KG, Li R, Mistrot C, Chen RH, Dann P, Rudner A, Murray AW. Hardwick KG, et al. Among authors: rudner a. Genetics. 1999 Jun;152(2):509-18. Genetics. 1999. PMID: 10353895 Free PMC article.
The spindle assembly checkpoint.
Rudner AD, Murray AW. Rudner AD, et al. Curr Opin Cell Biol. 1996 Dec;8(6):773-80. doi: 10.1016/s0955-0674(96)80077-9. Curr Opin Cell Biol. 1996. PMID: 8939672 Review.
A Wee1 checkpoint inhibits anaphase onset.
Lianga N, Williams EC, Kennedy EK, Doré C, Pilon S, Girard SL, Deneault JS, Rudner AD. Lianga N, et al. Among authors: rudner ad. J Cell Biol. 2013 Jun 10;201(6):843-62. doi: 10.1083/jcb.201212038. J Cell Biol. 2013. PMID: 23751495 Free PMC article.
The morphogenesis checkpoint also depended on Cdc55, a regulatory subunit of protein phosphatase 2A (PP2A). cdc55Δ checkpoint defects were rescued by mutating 12 Cdk1 phosphorylation sites on the APC, demonstrating that the APC is a target of this checkpoint. These …
The morphogenesis checkpoint also depended on Cdc55, a regulatory subunit of protein phosphatase 2A (PP2A). cdc55Δ checkpoint defects …
Redundant Regulation of Cdk1 Tyrosine Dephosphorylation in Saccharomyces cerevisiae.
Kennedy EK, Dysart M, Lianga N, Williams EC, Pilon S, Doré C, Deneault JS, Rudner AD. Kennedy EK, et al. Among authors: rudner ad. Genetics. 2016 Mar;202(3):903-10. doi: 10.1534/genetics.115.182469. Epub 2015 Dec 29. Genetics. 2016. PMID: 26715668 Free PMC article.
A novel proteomics approach for the discovery of chromatin-associated protein networks.
Lambert JP, Mitchell L, Rudner A, Baetz K, Figeys D. Lambert JP, et al. Among authors: rudner a. Mol Cell Proteomics. 2009 Apr;8(4):870-82. doi: 10.1074/mcp.M800447-MCP200. Epub 2008 Dec 22. Mol Cell Proteomics. 2009. PMID: 19106085 Free PMC article.
To address this problem, we have developed a novel method, termed modified chromatin immunopurification (mChIP), that allows for the efficient purification of protein-DNA macromolecules, enabling subsequent protein identification by mass spectrometry. mChIP consists of …
To address this problem, we have developed a novel method, termed modified chromatin immunopurification (mChIP), that allows for the …
Cdk1 phosphorylation of Esp1/Separase functions with PP2A and Slk19 to regulate pericentric Cohesin and anaphase onset.
Lianga N, Doré C, Kennedy EK, Yeh E, Williams EC, Fortinez CM, Wang A, Bloom KS, Rudner AD. Lianga N, et al. Among authors: rudner ad. PLoS Genet. 2018 Mar 21;14(3):e1007029. doi: 10.1371/journal.pgen.1007029. eCollection 2018 Mar. PLoS Genet. 2018. PMID: 29561844 Free PMC article.
Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex.
Rudner AD, Murray AW. Rudner AD, et al. J Cell Biol. 2000 Jun 26;149(7):1377-90. doi: 10.1083/jcb.149.7.1377. J Cell Biol. 2000. PMID: 10871279 Free PMC article.
This defect can be explained by a defect in APC phosphorylation, which depends on mitotic Cdc28 activity in vivo and can be catalyzed by purified Cdc28 in vitro. ...
This defect can be explained by a defect in APC phosphorylation, which depends on mitotic Cdc28 activity in vivo and can be catalyzed …
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