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The prosequence of thermolysin acts as an intramolecular chaperone when expressed in trans with the mature sequence in Escherichia coli.
Marie-Claire C, Ruffet E, Beaumont A, Roques BP. Marie-Claire C, et al. Among authors: ruffet e. J Mol Biol. 1999 Feb 5;285(5):1911-5. doi: 10.1006/jmbi.1998.2449. J Mol Biol. 1999. PMID: 9925774
Evidence by site-directed mutagenesis that arginine 203 of thermolysin and arginine 717 of neprilysin (neutral endopeptidase) play equivalent critical roles in substrate hydrolysis and inhibitor binding.
Marie-Claire C, Ruffet E, Antonczak S, Beaumont A, O'Donohue M, Roques BP, Fournié-Zaluski MC. Marie-Claire C, et al. Among authors: ruffet e. Biochemistry. 1997 Nov 11;36(45):13938-45. doi: 10.1021/bi9712495. Biochemistry. 1997. PMID: 9374873
Characterization of Glu350 as a critical residue involved in the N-terminal amine binding site of aminopeptidase N (EC 3.4.11.2): insights into its mechanism of action.
Luciani N, Marie-Claire C, Ruffet E, Beaumont A, Roques BP, Fournié-Zaluski MC. Luciani N, et al. Among authors: ruffet e. Biochemistry. 1998 Jan 13;37(2):686-92. doi: 10.1021/bi971705p. Biochemistry. 1998. PMID: 9425092
Exploration of the S(')(1) subsite of neprilysin: a joined molecular modeling and site-directed mutagenesis study.
Marie-Claire C, Tiraboschi G, Ruffet E, Inguimbert N, Fournie-Zaluski MC, Roques BP. Marie-Claire C, et al. Among authors: ruffet e. Proteins. 2000 Jun 1;39(4):365-71. doi: 10.1002/(sici)1097-0134(20000601)39:4<365::aid-prot90>3.0.co;2-t. Proteins. 2000. PMID: 10813818
Differences in transition state stabilization between thermolysin (EC 3.4.24.27) and neprilysin (EC 3.4.24.11).
Marie-Claire C, Ruffet E, Tiraboschi G, Fournie-Zaluski MC. Marie-Claire C, et al. Among authors: ruffet e. FEBS Lett. 1998 Nov 6;438(3):215-9. doi: 10.1016/s0014-5793(98)01267-8. FEBS Lett. 1998. PMID: 9827548 Free article.
Characterization of angiotensin IV-degrading enzymes and receptors on rat mesangial cells.
Chansel D, Czekalski S, Vandermeersch S, Ruffet E, Fournié-Zaluski MC, Ardaillou R. Chansel D, et al. Among authors: ruffet e. Am J Physiol. 1998 Oct;275(4):F535-42. doi: 10.1152/ajprenal.1998.275.4.F535. Am J Physiol. 1998. PMID: 9755125
High affinity serum-derived Fab fragments as another source of antibodies in the gut lumen of both neonates and adults.
Quan CP, Ruffet E, Arihiro K, Pires R, Bouvet JP. Quan CP, et al. Among authors: ruffet e. Scand J Immunol. 1996 Aug;44(2):108-14. doi: 10.1046/j.1365-3083.1996.d01-288.x. Scand J Immunol. 1996. PMID: 8711422
Activation of the classical pathway of complement by non-immune complexes of immunoglobulins with human protein FV (FV fragment-binding protein).
Ruffet E, Pirès R, Pillot J, Bouvet JP. Ruffet E, et al. Scand J Immunol. 1994 Sep;40(3):359-62. doi: 10.1111/j.1365-3083.1994.tb03474.x. Scand J Immunol. 1994. PMID: 8091137
MUC1 mucin gene, transcripts, and protein in adenomas and papillary carcinomas of the thyroid.
Bièche I, Ruffet E, Zweibaum A, Vildé F, Lidereau R, Franc B. Bièche I, et al. Among authors: ruffet e. Thyroid. 1997 Oct;7(5):725-31. doi: 10.1089/thy.1997.7.725. Thyroid. 1997. PMID: 9349575
A novel system for continuous protein refolding and on-line capture by expanded bed adsorption.
Ferré H, Ruffet E, Nielsen LL, Nissen MH, Hobley TJ, Thomas OR, Buus S. Ferré H, et al. Among authors: ruffet e. Protein Sci. 2005 Aug;14(8):2141-53. doi: 10.1110/ps.051396105. Protein Sci. 2005. PMID: 16046630 Free PMC article.
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