Abstract
A cDNA encoding the human substance P receptor (SPR) was isolated and the primary structure of the protein was deduced by nucleotide sequence analysis. This SPR consists of 407 residues and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SPR sequences demonstrated a 94.5% identity. The receptor was expressed in a COS-7 cell line and displayed a Kd for Tyr-1-SP binding of 0.24 nM. Ligand displacement by naturally occurring tachykinin peptides was SP much greater than neurokinin A greater than neurokinin B. SP stimulation of transfected cells resulted in a rapid and transient inositol 1,4,5-trisphosphate response. RNA blot hybridization and solution hybridization demonstrated that SPR mRNA was about 4.5 Kb in size, and was expressed in IM-9 lymphoblast and U373-MG astrocytoma cells, as well as in spinal cord and lung but not in liver.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding, Competitive
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Cell Line
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Cloning, Molecular / methods
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DNA / genetics
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DNA / isolation & purification
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Exons
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Humans
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Inositol 1,4,5-Trisphosphate / metabolism
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Kinetics
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction / methods
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RNA, Messenger / genetics
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Rats
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Receptors, Neurokinin-1
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Receptors, Neurotransmitter / genetics*
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Receptors, Neurotransmitter / metabolism
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Recombinant Proteins / metabolism
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Sequence Homology, Nucleic Acid
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Substance P / metabolism
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Transfection
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Neurokinin-1
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Receptors, Neurotransmitter
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Recombinant Proteins
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Substance P
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Inositol 1,4,5-Trisphosphate
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DNA
Associated data
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GENBANK/M74290
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GENBANK/M74785
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GENBANK/M74786
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GENBANK/M74787
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GENBANK/M74788
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GENBANK/M74790
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GENBANK/M81108
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GENBANK/M81109
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GENBANK/S57525
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GENBANK/S57527