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Year Number of Results
2004 1
2008 1
2009 1
2010 1
2011 3
2012 4
2013 3
2014 6
2015 3
2016 1
2017 2
2019 1
2020 2
2021 3
2022 0
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28 results
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Page 1
Establishment of a feeder and serum-free culture system for human embryonic stem cells.
Wang L, Zhang R, Ma R, Jia G, Jian S, Zeng X, Xiong Z, Li B, Li C, Lv Z, Bai X. Wang L, et al. Zygote. 2020 Jun;28(3):175-182. doi: 10.1017/S0967199419000625. Epub 2020 Jan 22. Zygote. 2020. PMID: 31965957
The aim of this study was to develop a feeder-free, serum-free culture method for human embryonic stem cells (hESCs), to establish optimal conditions for hESC proliferation, and to determine the biological characteristics of the resulting hESCs. ...The expres …
The aim of this study was to develop a feeder-free, serum-free culture method for human embryonic stem cells (hESCs), t …
In vitro culture and phenotypic and molecular characterization of gastric stem cells from human stomach.
Garcia M, Chomel JC, Mustapha P, Tran CT, Garnier M, Paris I, Quellard N, Godet J, Cremniter J, Bennaceur-Griscelli A, Lecron JC, Turhan AG, Burucoa C, Bodet C. Garcia M, et al. Helicobacter. 2017 Apr;22(2). doi: 10.1111/hel.12351. Epub 2016 Sep 4. Helicobacter. 2017. PMID: 27592706
The molecular characteristics of cells were studied using TaqMan low-density array and qRT-PCR analyses using the well-characterized H1 and H9 embryonic stem cells as reference. ...RESULTS: Resident gastric stem cells expressed spe …
The molecular characteristics of cells were studied using TaqMan low-density array and qRT-PCR analyses using the well-characterized …
Differences between human and mouse embryonic stem cells.
Ginis I, Luo Y, Miura T, Thies S, Brandenberger R, Gerecht-Nir S, Amit M, Hoke A, Carpenter MK, Itskovitz-Eldor J, Rao MS. Ginis I, et al. Dev Biol. 2004 May 15;269(2):360-80. doi: 10.1016/j.ydbio.2003.12.034. Dev Biol. 2004. PMID: 15110706 Free article.
We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis. ...Profound differences in cell cycle regulation, control of apoptosis, and cytokine expression were uncovered using focused mic …
We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA arra …
Self-Assembling Scaffolds Supported Long-Term Growth of Human Primed Embryonic Stem Cells and Upregulated Core and Naïve Pluripotent Markers.
McKee C, Brown C, Chaudhry GR. McKee C, et al. Cells. 2019 Dec 16;8(12):1650. doi: 10.3390/cells8121650. Cells. 2019. PMID: 31888235 Free PMC article.
The maintenance and expansion of human embryonic stem cells (ESCs) in two-dimensional (2-D) culture is technically challenging, requiring routine manipulation and passaging. ...Three-dimensional (3-D) self-assembling scaffold-grown cells displayed an u …
The maintenance and expansion of human embryonic stem cells (ESCs) in two-dimensional (2-D) culture is technically chal …
DJ-1 Can Replace FGF-2 for Long-Term Culture of Human Pluripotent Stem Cells in Defined Media and Feeder-Free Condition.
Kim J, Baek S, Hong YJ, de Paula MN, Jahan Prima M, Oh YM, Cha SS, Do JT, Jang YJ, Choe H. Kim J, et al. Int J Mol Sci. 2021 May 31;22(11):5954. doi: 10.3390/ijms22115954. Int J Mol Sci. 2021. PMID: 34073063 Free PMC article.
In this study, for the first time, we have replaced hFGF-2 with hDJ-1 in the essential eight media and maintained the human embryonic stem cells (hESCs), H9, in the defined media at feeder-free condition. After more than ten passages, H9 in both …
In this study, for the first time, we have replaced hFGF-2 with hDJ-1 in the essential eight media and maintained the human embryonic
Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells.
Wang X, Li S, Cao T, Fu X, Yu G. Wang X, et al. Toxicol In Vitro. 2012 Sep;26(6):1056-63. doi: 10.1016/j.tiv.2012.04.002. Epub 2012 Apr 16. Toxicol In Vitro. 2012. PMID: 22525295
To investigate the use of differentiated fibroblasts from human embryonic stem cells as a cellular model for cytotoxicity and genotoxicity screening. The EBf-H9 cells were derived from human embryonic stem cells (H9) …
To investigate the use of differentiated fibroblasts from human embryonic stem cells as a cellular model for cytotoxici …
Hematopoietic and endothelial differentiation of human induced pluripotent stem cells.
Choi KD, Yu J, Smuga-Otto K, Salvagiotto G, Rehrauer W, Vodyanik M, Thomson J, Slukvin I. Choi KD, et al. Stem Cells. 2009 Mar;27(3):559-67. doi: 10.1634/stemcells.2008-0922. Stem Cells. 2009. PMID: 19259936 Free PMC article.
Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well as for developing novel approaches for regenerative therapy based on immunologically compatible cells. In this study, we employed an O …
Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well a …
Establishment of clinically compliant human embryonic stem cells in an autologous feeder-free system.
Fu X, Toh WS, Liu H, Lu K, Li M, Cao T. Fu X, et al. Tissue Eng Part C Methods. 2011 Sep;17(9):927-37. doi: 10.1089/ten.TEC.2010.0735. Epub 2011 Jun 20. Tissue Eng Part C Methods. 2011. PMID: 21561302
Applications of human embryonic stem cells (hESCs) are limited by the use of mouse embryonic fibroblasts feeder and animal-derived components during culture. In this study, we demonstrated the potential use of extracellular matrix (ECM) derived from th …
Applications of human embryonic stem cells (hESCs) are limited by the use of mouse embryonic fibroblasts feeder …
One-step derivation of cardiomyocytes and mesenchymal stem cells from human pluripotent stem cells.
Wei H, Tan G, Manasi, Qiu S, Kong G, Yong P, Koh C, Ooi TH, Lim SY, Wong P, Gan SU, Shim W. Wei H, et al. Stem Cell Res. 2012 Sep;9(2):87-100. doi: 10.1016/j.scr.2012.04.003. Epub 2012 Apr 24. Stem Cell Res. 2012. PMID: 22683798 Free article.
Cardiomyocytes (CMs) and mesenchymal stem cells (MSCs) are important cell types for cardiac repair post myocardial infarction. ...Two hiPSC lines, hiPSC (1) and hiPSC (2) were generated from human dermal fibroblasts using OCT-4, SOX-2, KLF-4, c-Myc via retroviral-ba …
Cardiomyocytes (CMs) and mesenchymal stem cells (MSCs) are important cell types for cardiac repair post myocardial infarction. …
Continuous hypoxic culturing of human embryonic stem cells enhances SSEA-3 and MYC levels.
Närvä E, Pursiheimo JP, Laiho A, Rahkonen N, Emani MR, Viitala M, Laurila K, Sahla R, Lund R, Lähdesmäki H, Jaakkola P, Lahesmaa R. Närvä E, et al. PLoS One. 2013 Nov 13;8(11):e78847. doi: 10.1371/journal.pone.0078847. eCollection 2013. PLoS One. 2013. PMID: 24236059 Free PMC article.
Low oxygen tension (hypoxia) contributes critically to pluripotency of human embryonic stem cells (hESCs) by preventing spontaneous differentiation and supporting self-renewal. ...In this study we characterized the transcriptional and molecular responses of t …
Low oxygen tension (hypoxia) contributes critically to pluripotency of human embryonic stem cells (hESCs) by preventing …
28 results