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The construction and application of diploid sake yeast with a homozygous mutation in the FAS2 gene.
Kotaka A, Sahara H, Hata Y. Kotaka A, et al. Among authors: sahara h. J Biosci Bioeng. 2010 Dec;110(6):675-8. doi: 10.1016/j.jbiosc.2010.07.007. Epub 2010 Aug 12. J Biosci Bioeng. 2010. PMID: 20708434
Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity.
Kotaka A, Sahara H, Kondo A, Ueda M, Hata Y. Kotaka A, et al. Among authors: sahara h. Appl Microbiol Biotechnol. 2009 Feb;82(2):387-95. doi: 10.1007/s00253-008-1833-3. Epub 2009 Jan 10. Appl Microbiol Biotechnol. 2009. PMID: 19137286
Enhancement of display efficiency in yeast display system by vector engineering and gene disruption.
Kuroda K, Matsui K, Higuchi S, Kotaka A, Sahara H, Hata Y, Ueda M. Kuroda K, et al. Among authors: sahara h. Appl Microbiol Biotechnol. 2009 Mar;82(4):713-9. doi: 10.1007/s00253-008-1808-4. Epub 2009 Jan 3. Appl Microbiol Biotechnol. 2009. PMID: 19123001
Direct ethanol production from barley beta-glucan by sake yeast displaying Aspergillus oryzae beta-glucosidase and endoglucanase.
Kotaka A, Bando H, Kaya M, Kato-Murai M, Kuroda K, Sahara H, Hata Y, Kondo A, Ueda M. Kotaka A, et al. Among authors: sahara h. J Biosci Bioeng. 2008 Jun;105(6):622-7. doi: 10.1263/jbb.105.622. J Biosci Bioeng. 2008. PMID: 18640601
When direct ethanol fermentation from 20 g/l barley beta-glucan as a model substrate was performed with the codisplaying strain, the ethanol concentration reached 7.94 g/l after 24 h of fermentation. ...
When direct ethanol fermentation from 20 g/l barley beta-glucan as a model substrate was performed with the codisplaying strain, the ethanol …
Efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases.
Kotaka A, Sahara H, Hata Y, Abe Y, Kondo A, Kato-Murai M, Kuroda K, Ueda M. Kotaka A, et al. Among authors: sahara h. Biosci Biotechnol Biochem. 2008 May;72(5):1376-9. doi: 10.1271/bbb.70825. Epub 2008 May 7. Biosci Biotechnol Biochem. 2008. PMID: 18460787
Isoflavone aglycones production from isoflavone glycosides by display of beta-glucosidase from Aspergillus oryzae on yeast cell surface.
Kaya M, Ito J, Kotaka A, Matsumura K, Bando H, Sahara H, Ogino C, Shibasaki S, Kuroda K, Ueda M, Kondo A, Hata Y. Kaya M, et al. Among authors: sahara h. Appl Microbiol Biotechnol. 2008 May;79(1):51-60. doi: 10.1007/s00253-008-1393-6. Epub 2008 Mar 14. Appl Microbiol Biotechnol. 2008. PMID: 18340445
Global gene expression analysis of yeast cells during sake brewing.
Wu H, Zheng X, Araki Y, Sahara H, Takagi H, Shimoi H. Wu H, et al. Among authors: sahara h. Appl Environ Microbiol. 2006 Nov;72(11):7353-8. doi: 10.1128/AEM.01097-06. Epub 2006 Sep 22. Appl Environ Microbiol. 2006. PMID: 16997994 Free PMC article.
Enhancement of beta-glucosidase activity on the cell-surface of sake yeast by disruption of SED1.
Kotaka A, Sahara H, Kuroda K, Kondo A, Ueda M, Hata Y. Kotaka A, et al. Among authors: sahara h. J Biosci Bioeng. 2010 May;109(5):442-6. doi: 10.1016/j.jbiosc.2009.11.003. Epub 2009 Dec 4. J Biosci Bioeng. 2010. PMID: 20347765
Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate.
Sahara H, Kotaka A, Kondo A, Ueda M, Hata Y. Sahara H, et al. J Biosci Bioeng. 2009 Nov;108(5):359-64. doi: 10.1016/j.jbiosc.2009.05.002. J Biosci Bioeng. 2009. PMID: 19804856
Construction of an Aspergillus oryzae cell-surface display system using a putative GPI-anchored protein.
Adachi T, Ito J, Kawata K, Kaya M, Ishida H, Sahara H, Hata Y, Ogino C, Fukuda H, Kondo A. Adachi T, et al. Among authors: sahara h. Appl Microbiol Biotechnol. 2008 Dec;81(4):711-9. doi: 10.1007/s00253-008-1687-8. Epub 2008 Sep 24. Appl Microbiol Biotechnol. 2008. PMID: 18813924
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