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Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling.
Olafsen T, Bruland OS, Zalutsky MR, Sandlie I. Olafsen T, et al. Among authors: sandlie i. Nucl Med Biol. 1995 Aug;22(6):765-71. doi: 10.1016/0969-8051(95)00014-o. Nucl Med Biol. 1995. PMID: 8535337
Antibody dependent cell-mediated cytotoxicity induced by chimeric mouse-human IgG subclasses and IgG3 antibodies with altered hinge region.
Michaelsen TE, Aase A, Norderhaug L, Sandlie I. Michaelsen TE, et al. Among authors: sandlie i. Mol Immunol. 1992 Mar;29(3):319-26. doi: 10.1016/0161-5890(92)90018-s. Mol Immunol. 1992. PMID: 1557042
Chimeric mouse human IgG3 antibodies with an IgG4-like hinge region induce complement-mediated lysis more efficiently than IgG3 with normal hinge.
Norderhaug L, Brekke OH, Bremnes B, Sandin R, Aase A, Michaelsen TE, Sandlie I. Norderhaug L, et al. Among authors: sandlie i. Eur J Immunol. 1991 Oct;21(10):2379-84. doi: 10.1002/eji.1830211013. Eur J Immunol. 1991. PMID: 1915551
Enhancement of complement activation and cytolysis of human IgG3 by deletion of hinge exons.
Michaelsen TE, Aase A, Westby C, Sandlie I. Michaelsen TE, et al. Among authors: sandlie i. Scand J Immunol. 1990 Nov;32(5):517-28. doi: 10.1111/j.1365-3083.1990.tb03192.x. Scand J Immunol. 1990. PMID: 2125362
C1q binding to chimeric monoclonal IgG3 antibodies consisting of mouse variable regions and human constant regions with shortened hinge containing 15 to 47 amino acids.
Sandlie I, Aase A, Westby C, Michaelsen TE. Sandlie I, et al. Eur J Immunol. 1989 Sep;19(9):1599-603. doi: 10.1002/eji.1830190912. Eur J Immunol. 1989. PMID: 2792180
The structural requirements for complement activation by IgG: does it hinge on the hinge?
Brekke OH, Michaelsen TE, Sandlie I. Brekke OH, et al. Among authors: sandlie i. Immunol Today. 1995 Feb;16(2):85-90. doi: 10.1016/0167-5699(95)80094-8. Immunol Today. 1995. PMID: 7888072 Review.
As described here by Ole Henrik Brekke, Terje Michaelsen and Inger Sandlie, it now appears that the only requirement of the hinge region for complement activation is the presence of inter-heavy-chain disulfide bond(s). ...
As described here by Ole Henrik Brekke, Terje Michaelsen and Inger Sandlie, it now appears that the only requirement of the hinge reg …
One disulfide bond in front of the second heavy chain constant region is necessary and sufficient for effector functions of human IgG3 without a genetic hinge.
Michaelsen TE, Brekke OH, Aase A, Sandin RH, Bremnes B, Sandlie I. Michaelsen TE, et al. Among authors: sandlie i. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9243-7. doi: 10.1073/pnas.91.20.9243. Proc Natl Acad Sci U S A. 1994. PMID: 7937748 Free PMC article.
We have created four IgG3 mutants without a normal hinge region: (i) m0 without a genetic hinge; (ii) m0/C131S, where Cys-131 in m0 was mutated to Ser; (iii) m0/231C232 (formerly HM-1), where a Cys residue was inserted in m0 between Ala-231 and Pro-232; (iv) m0/C131S/231C2 …
We have created four IgG3 mutants without a normal hinge region: (i) m0 without a genetic hinge; (ii) m0/C131S, where Cys-131 in m0 w …
Human IgG3 can adopt the disulfide bond pattern characteristic for IgG1 without resembling it in complement mediated cell lysis.
Brekke OH, Bremnes B, Sandin R, Aase A, Michaelsen TE, Sandlie I. Brekke OH, et al. Among authors: sandlie i. Mol Immunol. 1993 Nov;30(16):1419-25. doi: 10.1016/0161-5890(93)90103-i. Mol Immunol. 1993. PMID: 8232327
The extended hinge region of IgG3 is not required for high phagocytic capacity mediated by Fc gamma receptors, but the heavy chains must be disulfide bonded.
Aase A, Sandlie I, Norderhaug L, Brekke OH, Michaelsen TE. Aase A, et al. Among authors: sandlie i. Eur J Immunol. 1993 Jul;23(7):1546-51. doi: 10.1002/eji.1830230723. Eur J Immunol. 1993. PMID: 8325331
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