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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
Schägger H, von Jagow G. Schägger H, et al. Anal Biochem. 1987 Nov 1;166(2):368-79. doi: 10.1016/0003-2697(87)90587-2. Anal Biochem. 1987. PMID: 2449095
Isolation, characterization and electron microscopic single particle analysis of the NADH:ubiquinone oxidoreductase (complex I) from the hyperthermophilic eubacterium Aquifex aeolicus.
Peng G, Fritzsch G, Zickermann V, Schägger H, Mentele R, Lottspeich F, Bostina M, Radermacher M, Huber R, Stetter KO, Michel H. Peng G, et al. Among authors: schagger h. Biochemistry. 2003 Mar 18;42(10):3032-9. doi: 10.1021/bi026876v. Biochemistry. 2003. PMID: 12627969
The isolated complex is highly stable and active in a temperature range from 50 to 90 degrees C, with a half-life of about 10 h at 80 degrees C. ...
The isolated complex is highly stable and active in a temperature range from 50 to 90 degrees C, with a half-life of about 10 h at 80 …
Tricine-SDS-PAGE.
Schägger H. Schägger H. Nat Protoc. 2006;1(1):16-22. doi: 10.1038/nprot.2006.4. Nat Protoc. 2006. PMID: 17406207
Blue native PAGE.
Wittig I, Braun HP, Schägger H. Wittig I, et al. Among authors: schagger h. Nat Protoc. 2006;1(1):418-28. doi: 10.1038/nprot.2006.62. Nat Protoc. 2006. PMID: 17406264
Mass estimation of native proteins by blue native electrophoresis: principles and practical hints.
Wittig I, Beckhaus T, Wumaier Z, Karas M, Schägger H. Wittig I, et al. Among authors: schagger h. Mol Cell Proteomics. 2010 Oct;9(10):2149-61. doi: 10.1074/mcp.M900526-MCP200. Epub 2010 Feb 20. Mol Cell Proteomics. 2010. PMID: 20173216 Free PMC article.
Chapter 8 Two-dimensional native electrophoresis for fluorescent and functional assays of mitochondrial complexes.
Wumaier Z, Nübel E, Wittig I, Schägger H. Wumaier Z, et al. Among authors: schagger h. Methods Enzymol. 2009;456:153-68. doi: 10.1016/S0076-6879(08)04408-X. Methods Enzymol. 2009. PMID: 19348887
Features and applications of blue-native and clear-native electrophoresis.
Wittig I, Schägger H. Wittig I, et al. Among authors: schagger h. Proteomics. 2008 Oct;8(19):3974-90. doi: 10.1002/pmic.200800017. Proteomics. 2008. PMID: 18763698 Review.
High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes.
Wittig I, Karas M, Schägger H. Wittig I, et al. Among authors: schagger h. Mol Cell Proteomics. 2007 Jul;6(7):1215-25. doi: 10.1074/mcp.M700076-MCP200. Epub 2007 Apr 9. Mol Cell Proteomics. 2007. PMID: 17426019
Advantages and limitations of clear-native PAGE.
Wittig I, Schägger H. Wittig I, et al. Among authors: schagger h. Proteomics. 2005 Nov;5(17):4338-46. doi: 10.1002/pmic.200500081. Proteomics. 2005. PMID: 16220535
Two-dimensional electrophoresis for the isolation of integral membrane proteins and mass spectrometric identification.
Rais I, Karas M, Schägger H. Rais I, et al. Among authors: schagger h. Proteomics. 2004 Sep;4(9):2567-71. doi: 10.1002/pmic.200400829. Proteomics. 2004. PMID: 15352231
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