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Towards maximum acceleration of monoclonal antibody development: Leveraging transposase-mediated cell line generation to enable GMP manufacturing within 3 months using a stable pool.
Schmieder V, Fieder J, Drerup R, Gutierrez EA, Guelch C, Stolzenberger J, Stumbaum M, Mueller VS, Higel F, Bergbauer M, Bornhoefft K, Wittner M, Gronemeyer P, Braig C, Huber M, Reisenauer-Schaupp A, Mueller MM, Schuette M, Puengel S, Lindner B, Schmidt M, Schulz P, Fischer S. Schmieder V, et al. J Biotechnol. 2022 Apr 10;349:53-64. doi: 10.1016/j.jbiotec.2022.03.010. Epub 2022 Mar 24. J Biotechnol. 2022. PMID: 35341894 Free article.
Loss of a newly discovered microRNA in Chinese hamster ovary cells leads to upregulation of N-glycolylneuraminic acid sialylation on monoclonal antibodies.
Fischer S, Mathias S, Stadermann A, Yang S, Schmieder V, Zeh N, Schmidt N, Richter P, Wright S, Zimmermann E, Ley Y, van der Meer J, Hartsch T, Bernloehr C, Otte K, Bradl H, Gamer M, Schulz P. Fischer S, et al. Among authors: schmieder v. Biotechnol Bioeng. 2022 Mar;119(3):832-844. doi: 10.1002/bit.28015. Epub 2022 Jan 14. Biotechnol Bioeng. 2022. PMID: 34935124 Free PMC article.