R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays.
Scholz C, Maier P, Dolinski K, Heitman J, Schmid FX.
Scholz C, et al.
FEBS Lett. 1999 Jan 29;443(3):367-9. doi: 10.1016/s0014-5793(98)01735-9.
FEBS Lett. 1999.
PMID: 10025965
Free article.
Previously we reported that the R73A and H144Q variants of the yeast cyclophilin Cpr3 were virtually inactive in a protease-coupled peptide assay, but retained activity as catalysts of a proline-limited protein folding reaction [Scholz, C. et al. (1997) FEBS Lett. 4 …
Previously we reported that the R73A and H144Q variants of the yeast cyclophilin Cpr3 were virtually inactive in a protease-coupled peptide …