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A PCR-Based Method to Genotype Mice Knocked Out for All Four CD3 Subunits, the Standard Recipient Strain for Retrogenic TCR/CD3 Bone Marrow Reconstitution Technology.
Ferrer A, Schrum AG, Gil D. Ferrer A, et al. Among authors: schrum ag. Biores Open Access. 2013 Jun;2(3):222-6. doi: 10.1089/biores.2013.0002. Biores Open Access. 2013. PMID: 23741635 Free PMC article.
IL-12 Signals through the TCR To Support CD8 Innate Immune Responses.
Goplen NP, Saxena V, Knudson KM, Schrum AG, Gil D, Daniels MA, Zamoyska R, Teixeiro E. Goplen NP, et al. Among authors: schrum ag. J Immunol. 2016 Sep 15;197(6):2434-43. doi: 10.4049/jimmunol.1600037. Epub 2016 Aug 12. J Immunol. 2016. PMID: 27521342 Free PMC article.
Basal and antigen-induced exposure of the proline-rich sequence in CD3ε.
de la Cruz J, Kruger T, Parks CA, Silge RL, van Oers NS, Luescher IF, Schrum AG, Gil D. de la Cruz J, et al. Among authors: schrum ag. J Immunol. 2011 Feb 15;186(4):2282-90. doi: 10.4049/jimmunol.1003225. Epub 2011 Jan 12. J Immunol. 2011. PMID: 21228347 Free PMC article.
Ag presentation in vivo induced open-CD3, accounting for half of the basal level found in thymocytes from MHC(+) mice. Additional stimulation with either anti-CD3 Abs or peptide-MHC ligands further elevated open-CD3 above basal levels, consistent with a model wherein antig
Ag presentation in vivo induced open-CD3, accounting for half of the basal level found in thymocytes from MHC(+) mice. Additional sti
A role for CD8 in the developmental tuning of antigen recognition and CD3 conformational change.
Gil D, Schrum AG, Daniels MA, Palmer E. Gil D, et al. Among authors: schrum ag. J Immunol. 2008 Mar 15;180(6):3900-9. doi: 10.4049/jimmunol.180.6.3900. J Immunol. 2008. PMID: 18322198 Free PMC article.
Interestingly, neither enhancement of Ag binding strength nor Src kinase signaling explained this coreceptor activity. Furthermore, Ag-induced CD3Deltac was developmentally attenuated by the increase in sialylation that accompanies T cell maturation and limits CD8 a …
Interestingly, neither enhancement of Ag binding strength nor Src kinase signaling explained this coreceptor activity. Furthermore, …
A co-housing strategy to improve fecundity of mice in timed matings.
Stiles RJ, Schrum AG, Gil D. Stiles RJ, et al. Among authors: schrum ag. Lab Anim (NY). 2013 Feb;42(2):62-5. doi: 10.1038/laban.161. Lab Anim (NY). 2013. PMID: 23340789 Free PMC article.
Detection of constant domain of human T cell antigen receptor alpha-chain via novel monoclonal antibody 7F18.
Reed BK, Lee KA, Bell MP, Gil D, Schrum AG. Reed BK, et al. Among authors: schrum ag. Monoclon Antib Immunodiagn Immunother. 2014 Dec;33(6):386-92. doi: 10.1089/mab.2013.0086. Monoclon Antib Immunodiagn Immunother. 2014. PMID: 25545207 Free PMC article.
IgG Fab fragments forming bivalent complexes by a conformational mechanism that is reversible by osmolytes.
Nelson AD, Hoffmann MM, Parks CA, Dasari S, Schrum AG, Gil D. Nelson AD, et al. Among authors: schrum ag. J Biol Chem. 2012 Dec 14;287(51):42936-50. doi: 10.1074/jbc.M112.410217. Epub 2012 Oct 29. J Biol Chem. 2012. PMID: 23109335 Free PMC article.
Physical and functional bivalency observed among TCR/CD3 complexes isolated from primary T cells.
Schrum AG, Gil D, Turka LA, Palmer E. Schrum AG, et al. J Immunol. 2011 Jul 15;187(2):870-8. doi: 10.4049/jimmunol.1100538. Epub 2011 Jun 10. J Immunol. 2011. PMID: 21666056 Free PMC article.
The expression of bivalent TCR/CD3 complexes has implications regarding potential mechanisms by which Ag may trigger signaling. It also suggests the possibility that the potential for bivalent expression could represent a general feature of Ag receptors....
The expression of bivalent TCR/CD3 complexes has implications regarding potential mechanisms by which Ag may trigger signaling. It al …
Multiplex matrix network analysis of protein complexes in the human TCR signalosome.
Smith SE, Neier SC, Reed BK, Davis TR, Sinnwell JP, Eckel-Passow JE, Sciallis GF, Wieland CN, Torgerson RR, Gil D, Neuhauser C, Schrum AG. Smith SE, et al. Among authors: schrum ag. Sci Signal. 2016 Aug 2;9(439):rs7. doi: 10.1126/scisignal.aad7279. Sci Signal. 2016. PMID: 27485017 Free PMC article.
Strategies to stabilize compact folding and minimize aggregation of antibody-based fragments.
Gil D, Schrum AG. Gil D, et al. Among authors: schrum ag. Adv Biosci Biotechnol. 2013 Apr;4(4a):73-84. doi: 10.4236/abb.2013.44A011. Adv Biosci Biotechnol. 2013. PMID: 25635232 Free PMC article.
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