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Selecting the optimal time to perform biopsy for preimplantation genetic testing.
Scott KL, Hong KH, Scott RT Jr. Scott KL, et al. Fertil Steril. 2013 Sep;100(3):608-14. doi: 10.1016/j.fertnstert.2013.07.004. Fertil Steril. 2013. PMID: 23993663 Review.
Selecting the optimal time for biopsy requires careful consideration. ...At present the blastocyst stage is the optimal time to perform biopsies for preimplantation genetic testing....
Selecting the optimal time for biopsy requires careful consideration. ...At present the blastocyst stage is the
Human Blastocyst Biopsy and Vitrification.
Maggiulli R, Giancani A, Cimadomo D, Ubaldi FM, Rienzi L. Maggiulli R, et al. J Vis Exp. 2019 Jul 26;(149). doi: 10.3791/59625. J Vis Exp. 2019. PMID: 31403619
Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation genetic testing. Then, the ideal workflow entails a safe and efficient vitrification protocol, due to the turnaround time of the …
Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation
Optimal timing of blastocyst vitrification after trophectoderm biopsy for preimplantation genetic screening.
Chen HH, Huang CC, Cheng EH, Lee TH, Chien LF, Lee MS. Chen HH, et al. PLoS One. 2017 Oct 5;12(10):e0185747. doi: 10.1371/journal.pone.0185747. eCollection 2017. PLoS One. 2017. PMID: 28982142 Free PMC article.
The PGS and frozen blastocyst transfer (FET) cycles were performed from December 2012 to May 2015. Only the good quality and expanded blastocysts on day 5 or 6 were selected for biopsy. ...All biopsied blastocysts were subjected to vitrification within …
The PGS and frozen blastocyst transfer (FET) cycles were performed from December 2012 to May 2015. Only the good quality and expanded …
Comprehensive chromosome screening and gene expression analysis from the same biopsy in human preimplantation embryos.
Marin D, Wang Y, Tao X, Scott RT Jr, Treff NR. Marin D, et al. Mol Hum Reprod. 2017 May 1;23(5):330-338. doi: 10.1093/molehr/gax014. Mol Hum Reprod. 2017. PMID: 28369516 Free PMC article.
STUDY QUESTION: Can simultaneous comprehensive chromosome screening (CCS) and gene expression analysis be performed on the same biopsy of preimplantation human embryos? SUMMARY ANSWER: For the first time, CCS and reliable gene expression analysis have …
STUDY QUESTION: Can simultaneous comprehensive chromosome screening (CCS) and gene expression analysis be performed on the same bi
A generic, flexible protocol for preimplantation human leukocyte antigen typing alone or in combination with a monogenic disease, for rapid case work-up and application.
Kakourou G, Destouni A, Vrettou C, Traeger-Synodinos J, Kanavakis E. Kakourou G, et al. Hemoglobin. 2014;38(1):49-55. doi: 10.3109/03630269.2013.842582. Epub 2013 Oct 16. Hemoglobin. 2014. PMID: 24131134
Human leukocyte antigen (HLA) typing of in vitro fertilization (IVF) embryos, aims to establish a pregnancy that is HLA compatible with an affected sibling who requires hematopoietic stem cell transplantation (HSCT). It can be performed with or without preimplantation
Human leukocyte antigen (HLA) typing of in vitro fertilization (IVF) embryos, aims to establish a pregnancy that is HLA compatible with an a …
Obtaining metaphase spreads from single blastomeres for PGD of chromosomal rearrangements.
Shkumatov A, Kuznyetsov V, Cieslak J, Ilkevitch Y, Verlinsky Y. Shkumatov A, et al. Reprod Biomed Online. 2007 Apr;14(4):498-503. doi: 10.1016/s1472-6483(10)60899-1. Reprod Biomed Online. 2007. PMID: 17425834
The substances tested were calyculin A, caffeine, paclitaxel and colcemid in a total of 496 disaggregated and 234 biopsied blastomeres from day 3 embryos. It was demonstrated that the optimal method involved a combined use of 'selective biopsy' (sel
The substances tested were calyculin A, caffeine, paclitaxel and colcemid in a total of 496 disaggregated and 234 biopsied blastomere …