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Selecting the optimal time to perform biopsy for preimplantation genetic testing.
Scott KL, Hong KH, Scott RT Jr. Scott KL, et al. Fertil Steril. 2013 Sep;100(3):608-14. doi: 10.1016/j.fertnstert.2013.07.004. Fertil Steril. 2013. PMID: 23993663 Free article. Review.
Selecting the optimal time for biopsy requires careful consideration. ...At present the blastocyst stage is the optimal time to perform biopsies for preimplantation genetic testing....
Selecting the optimal time for biopsy requires careful consideration. ...At present the blastocyst stage is the …
Optimal timing of blastocyst vitrification after trophectoderm biopsy for preimplantation genetic screening.
Chen HH, Huang CC, Cheng EH, Lee TH, Chien LF, Lee MS. Chen HH, et al. PLoS One. 2017 Oct 5;12(10):e0185747. doi: 10.1371/journal.pone.0185747. eCollection 2017. PLoS One. 2017. PMID: 28982142 Free PMC article.
The PGS and frozen blastocyst transfer (FET) cycles were performed from December 2012 to May 2015. Only the good quality and expanded blastocysts on day 5 or 6 were selected for biopsy. ...All biopsied blastocysts were subjected to vitrification within …
The PGS and frozen blastocyst transfer (FET) cycles were performed from December 2012 to May 2015. Only the good quality and expanded …
Human Blastocyst Biopsy and Vitrification.
Maggiulli R, Giancani A, Cimadomo D, Ubaldi FM, Rienzi L. Maggiulli R, et al. J Vis Exp. 2019 Jul 26;(149). doi: 10.3791/59625. J Vis Exp. 2019. PMID: 31403619
Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation genetic testing. Then, the ideal workflow entails a safe and efficient vitrification protocol, due to the turnaround time of the …
Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation …
Validation of Non-Invasive Preimplantation Genetic Screening Using a Routine IVF Laboratory Workflow.
Tsai NC, Chang YC, Su YR, Lin YC, Weng PL, Cheng YH, Li YL, Lan KC. Tsai NC, et al. Biomedicines. 2022 Jun 11;10(6):1386. doi: 10.3390/biomedicines10061386. Biomedicines. 2022. PMID: 35740408 Free PMC article.
Embryo selection is needed to optimize the chances of pregnancy in assisted reproduction technology. ...Can niPGT-A combined with time-lapse morphokinetics provide a better embryo-selection strategy? ...
Embryo selection is needed to optimize the chances of pregnancy in assisted reproduction technology. ...Can niPGT-A combined w …
Is there an optimal window of time for transferring single frozen-thawed euploid blastocysts? A cohort study of 1170 embryo transfers.
An BGL, Chapman M, Tilia L, Venetis C. An BGL, et al. Hum Reprod. 2022 Nov 24;37(12):2797-2807. doi: 10.1093/humrep/deac227. Hum Reprod. 2022. PMID: 36305795
STUDY QUESTION: Is there an optimal window of time when the transfer of single frozen-thawed euploid blastocysts is associated with a maximal live birth rate (LBR)? ...STUDY DESIGN, SIZE, DURATION: This is a multicentered retrospective cohort study analyzing 1170 si …
STUDY QUESTION: Is there an optimal window of time when the transfer of single frozen-thawed euploid blastocysts is associated …
The expression of pregnancy-associated plasma protein-A (PAPP-A) in human blastocoel fluid-conditioned media: a proof of concept study.
Kavoussi SK, Chen SH, Wininger JD, Lal A, Roudebush WE, Lanford HC, Esqueda AS, Barsky M, Lebovic DI, Kavoussi PK, Gilkey MS, Chen J, Machen GL, Chosed RJ. Kavoussi SK, et al. J Assist Reprod Genet. 2022 Feb;39(2):389-394. doi: 10.1007/s10815-022-02393-4. Epub 2022 Jan 11. J Assist Reprod Genet. 2022. PMID: 35013837 Free PMC article.
BFCM was obtained from blastocyst stage embryos in 20 uL drops. Blastocysts underwent trophectoderm biopsy for preimplantation genetic testing for aneuploidy prior to blastocyst vitrification and BFCM collection for snap freezing. cfDNA was synthesized using …
BFCM was obtained from blastocyst stage embryos in 20 uL drops. Blastocysts underwent trophectoderm biopsy for preimplantation …
Comprehensive chromosome screening and gene expression analysis from the same biopsy in human preimplantation embryos.
Marin D, Wang Y, Tao X, Scott RT Jr, Treff NR. Marin D, et al. Mol Hum Reprod. 2017 May 1;23(5):330-338. doi: 10.1093/molehr/gax014. Mol Hum Reprod. 2017. PMID: 28369516 Free PMC article.
STUDY QUESTION: Can simultaneous comprehensive chromosome screening (CCS) and gene expression analysis be performed on the same biopsy of preimplantation human embryos? SUMMARY ANSWER: For the first time, CCS and reliable gene expression analysis have …
STUDY QUESTION: Can simultaneous comprehensive chromosome screening (CCS) and gene expression analysis be performed on the same bi …
A generic, flexible protocol for preimplantation human leukocyte antigen typing alone or in combination with a monogenic disease, for rapid case work-up and application.
Kakourou G, Destouni A, Vrettou C, Traeger-Synodinos J, Kanavakis E. Kakourou G, et al. Hemoglobin. 2014;38(1):49-55. doi: 10.3109/03630269.2013.842582. Epub 2013 Oct 16. Hemoglobin. 2014. PMID: 24131134
Human leukocyte antigen (HLA) typing of in vitro fertilization (IVF) embryos, aims to establish a pregnancy that is HLA compatible with an affected sibling who requires hematopoietic stem cell transplantation (HSCT). It can be performed with or without preimplantation …
Human leukocyte antigen (HLA) typing of in vitro fertilization (IVF) embryos, aims to establish a pregnancy that is HLA compatible with an a …
Obtaining metaphase spreads from single blastomeres for PGD of chromosomal rearrangements.
Shkumatov A, Kuznyetsov V, Cieslak J, Ilkevitch Y, Verlinsky Y. Shkumatov A, et al. Reprod Biomed Online. 2007 Apr;14(4):498-503. doi: 10.1016/s1472-6483(10)60899-1. Reprod Biomed Online. 2007. PMID: 17425834
The substances tested were calyculin A, caffeine, paclitaxel and colcemid in a total of 496 disaggregated and 234 biopsied blastomeres from day 3 embryos. It was demonstrated that the optimal method involved a combined use of 'selective biopsy' (sel …
The substances tested were calyculin A, caffeine, paclitaxel and colcemid in a total of 496 disaggregated and 234 biopsied blastomere …