Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1998 1
2001 1
2005 1
2006 2
2007 1
2008 1
2009 1
2010 1
2011 1
2014 1
2015 2
2018 2
2019 1
2023 0

Text availability

Article attribute

Article type

Publication date

Search Results

14 results

Results by year

Citations

1 article found by citation matching

Search results

Filters applied: . Clear all
Page 1
Non-animal methods to predict skin sensitization (I): the Cosmetics Europe database<sup/>
Hoffmann S, Kleinstreuer N, Alépée N, Allen D, Api AM, Ashikaga T, Clouet E, Cluzel M, Desprez B, Gellatly N, Goebel C, Kern PS, Klaric M, Kühnl J, Lalko JF, Martinozzi-Teissier S, Mewes K, Miyazawa M, Parakhia R, van Vliet E, Zang Q, Petersohn D. Hoffmann S, et al. Crit Rev Toxicol. 2018 May;48(5):344-358. doi: 10.1080/10408444.2018.1429385. Epub 2018 Feb 23. Crit Rev Toxicol. 2018. PMID: 29474128 Review.
Based on a systematic evaluation of test methods for skin sensitization, five non-animal test methods (DPRA (Direct Peptide Reactivity Assay), KeratinoSens(TM), h-CLAT (human cell line activation test), U-SENS(TM), SENS-IS) …
Based on a systematic evaluation of test methods for skin sensitization, five non-animal test m …
A comparative evaluation of in vitro skin sensitisation tests: the human cell-line activation test (h-CLAT) versus the local lymph node assay (LLNA).
Ashikaga T, Sakaguchi H, Sono S, Kosaka N, Ishikawa M, Nukada Y, Miyazawa M, Ito Y, Nishiyama N, Itagaki H. Ashikaga T, et al. Altern Lab Anim. 2010 Aug;38(4):275-84. doi: 10.1177/026119291003800403. Altern Lab Anim. 2010. PMID: 20822320
We previously developed the human cell-line activation test (h-CLAT) in vitro skin sensitisation test, based on our reported finding that a 24-hour exposure of THP-1 cells (a human monocytic leukaemia cell line) to sensitisers is s …
We previously developed the human cell-line activation test (h-CLAT) in vitro skin sensitisation test …
Predictive performance for human skin sensitizing potential of the human cell line activation test (h-CLAT).
Nukada Y, Ashikaga T, Sakaguchi H, Sono S, Mugita N, Hirota M, Miyazawa M, Ito Y, Sasa H, Nishiyama N. Nukada Y, et al. Contact Dermatitis. 2011 Dec;65(6):343-53. doi: 10.1111/j.1600-0536.2011.01952.x. Epub 2011 Jul 18. Contact Dermatitis. 2011. PMID: 21767275
BACKGROUND: Recent changes in regulatory restrictions and social opposition to animal toxicology experiments have driven the need for reliable in vitro tests for predicting the skin sensitizing potentials of a wide variety of industrial chemicals …
BACKGROUND: Recent changes in regulatory restrictions and social opposition to animal toxicology experiments have driven the need for reliab …
A cell-based in vitro alternative to identify skin sensitizers by gene expression.
Hooyberghs J, Schoeters E, Lambrechts N, Nelissen I, Witters H, Schoeters G, Van Den Heuvel R. Hooyberghs J, et al. Toxicol Appl Pharmacol. 2008 Aug 15;231(1):103-11. doi: 10.1016/j.taap.2008.03.014. Epub 2008 Apr 4. Toxicol Appl Pharmacol. 2008. PMID: 18466943
The ethical and economic burden associated with animal testing for assessment of skin sensitization has triggered intensive research effort towards development and validation of alternative methods. ...These results show that the present model m …
The ethical and economic burden associated with animal testing for assessment of skin sensitization has triggered inten …
Test battery with the human cell line activation test, direct peptide reactivity assay and DEREK based on a 139 chemical data set for predicting skin sensitizing potential and potency of chemicals.
Takenouchi O, Fukui S, Okamoto K, Kurotani S, Imai N, Fujishiro M, Kyotani D, Kato Y, Kasahara T, Fujita M, Toyoda A, Sekiya D, Watanabe S, Seto H, Hirota M, Ashikaga T, Miyazawa M. Takenouchi O, et al. J Appl Toxicol. 2015 Nov;35(11):1318-32. doi: 10.1002/jat.3127. Epub 2015 Mar 29. J Appl Toxicol. 2015. PMID: 25820183
To develop a testing strategy incorporating the human cell line activation test (h-CLAT), direct peptide reactivity assay (DPRA) and DEREK, we created an expanded data set of 139 chemicals (102 sensitizers and 37 non-sensitizers) by combi …
To develop a testing strategy incorporating the human cell line activation test (h-CLAT), direct peptide reactivity ass …
Probabilistic hazard assessment for skin sensitization potency by dose-response modeling using feature elimination instead of quantitative structure-activity relationships.
Luechtefeld T, Maertens A, McKim JM, Hartung T, Kleensang A, Sá-Rocha V. Luechtefeld T, et al. J Appl Toxicol. 2015 Nov;35(11):1361-1371. doi: 10.1002/jat.3172. Epub 2015 Jun 5. J Appl Toxicol. 2015. PMID: 26046447 Free PMC article.
We curated such a data set and combined a recursive variable selection algorithm to evaluate the information available through in silico, in chemico and in vitro assays. Chemical similarity alone could not cluster chemicals' potency, and in v …
We curated such a data set and combined a recursive variable selection algorithm to evaluate the information available through …
Predicting full thickness skin sensitization using a support vector machine.
Lee S, Dong DX, Jindal R, Maguire T, Mitra B, Schloss R, Yarmush M. Lee S, et al. Toxicol In Vitro. 2014 Dec;28(8):1413-23. doi: 10.1016/j.tiv.2014.07.002. Epub 2014 Jul 12. Toxicol In Vitro. 2014. PMID: 25025180 Free PMC article.
However, given the underlying complexity of ACD, one cell type and limited cellular metrics may be insufficient to predict contact sensitizers accurately. To identify a molecular signature that can further characterize sensitization, we developed a novel syst …
However, given the underlying complexity of ACD, one cell type and limited cellular metrics may be insufficient to predict contact sensit …
Development of an in vitro skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol.
Ashikaga T, Yoshida Y, Hirota M, Yoneyama K, Itagaki H, Sakaguchi H, Miyazawa M, Ito Y, Suzuki H, Toyoda H. Ashikaga T, et al. Toxicol In Vitro. 2006 Aug;20(5):767-73. doi: 10.1016/j.tiv.2005.10.012. Epub 2005 Nov 28. Toxicol In Vitro. 2006. PMID: 16311011
The aim of this study is to optimize the experimental conditions for an in vitro skin sensitization test using the human cell lines THP-1 and U-937. ...We propose to call this kind of in vitro skin sensitization test h-CLAT, …
The aim of this study is to optimize the experimental conditions for an in vitro skin sensitization test using t …
Industry experience with alternative methods.
LeClaire J, de Silva O. LeClaire J, et al. Toxicol Lett. 1998 Dec 28;102-103:575-9. doi: 10.1016/s0378-4274(98)00251-3. Toxicol Lett. 1998. PMID: 10022316
Alternative methods are used daily to assess eye and skin tolerance, phototoxicity, photoprotection, skin sensitization, percutaneous absorption and skin and hair care. For eye irritation, many years of in-house studies have enabled us to dev …
Alternative methods are used daily to assess eye and skin tolerance, phototoxicity, photoprotection, skin sensitizat …
14 results