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Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection.
Seydel A, Gounon P, Pugsley AP. Seydel A, et al. Mol Microbiol. 1999 Nov;34(4):810-21. doi: 10.1046/j.1365-2958.1999.01647.x. Mol Microbiol. 1999. PMID: 10564520
We report a novel strategy for selecting mutations that mislocalize lipoproteins within the Escherichia coli cell envelope and describe the mutants obtained. A strain carrying a deletion of the chromosomal malE gene, coding for the periplasmic maltose-binding …
We report a novel strategy for selecting mutations that mislocalize lipoproteins within the Escherichia coli cell envelope and descri …
The secretin-specific, chaperone-like protein of the general secretory pathway: separation of proteolytic protection and piloting functions.
Hardie KR, Seydel A, Guilvout I, Pugsley AP. Hardie KR, et al. Among authors: seydel a. Mol Microbiol. 1996 Dec;22(5):967-76. doi: 10.1046/j.1365-2958.1996.01539.x. Mol Microbiol. 1996. PMID: 8971717
The MalE-PulS hybrid was rendered completely functional (i.e. it restored pullulanase secretion in a pulS mutant) by replacing its signal peptide with a lipoprotein-type signal peptide. However, this fattyacylated hybrid protein was only functional if it also carrie …
The MalE-PulS hybrid was rendered completely functional (i.e. it restored pullulanase secretion in a pulS mutant) by replacing its si …
Pullulanase: model protein substrate for the general secretory pathway of gram-negative bacteria.
Pugsley AP, Francetic O, Hardie K, Possot OM, Sauvonnet N, Seydel A. Pugsley AP, et al. Among authors: seydel a. Folia Microbiol (Praha). 1997;42(3):184-92. doi: 10.1007/BF02818976. Folia Microbiol (Praha). 1997. PMID: 9246760 Review.
Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by the complex main terminal branch (MTB) of the general secretory pathway. ...In this review it is proposed that most of these proteins form …
Pullulanase of Klebsiella oxytoca is one of a wide variety of extracellular proteins that are secreted by Gram-negative bacteria by t …
Crystal structure of CagZ, a protein from the Helicobacter pylori pathogenicity island that encodes for a type IV secretion system.
Cendron L, Seydel A, Angelini A, Battistutta R, Zanotti G. Cendron L, et al. Among authors: seydel a. J Mol Biol. 2004 Jul 16;340(4):881-9. doi: 10.1016/j.jmb.2004.05.016. J Mol Biol. 2004. PMID: 15223328
The protein consists of a single compact L-shaped domain, composed of seven alpha-helices including about 70% of the total residues. Three-dimensional homology searches did not reveal structural homologues, and CagZ can be considered representative of a new protein …
The protein consists of a single compact L-shaped domain, composed of seven alpha-helices including about 70% of the total residues. …
Expression, purification and characterisation of the C-terminal STAS domain of the SLC26 anion transporter prestin.
Pasqualetto E, Seydel A, Pellini A, Battistutta R. Pasqualetto E, et al. Among authors: seydel a. Protein Expr Purif. 2008 Apr;58(2):249-56. doi: 10.1016/j.pep.2007.12.006. Epub 2007 Dec 25. Protein Expr Purif. 2008. PMID: 18226918
The two proteins possess a three-dimensional structure and show a great tendency to aggregate. In particular, PreCD(L) is present in solution mainly as dimers and tetramers. ...
The two proteins possess a three-dimensional structure and show a great tendency to aggregate. In particular, PreCD(L) is pres …
The crystal structure of CagS from the Helicobacter pylori pathogenicity island.
Cendron L, Tasca E, Seraglio T, Seydel A, Angelini A, Battistutta R, Montecucco C, Zanotti G. Cendron L, et al. Among authors: seydel a. Proteins. 2007 Nov 1;69(2):440-3. doi: 10.1002/prot.21576. Proteins. 2007. PMID: 17623849 No abstract available.
Characterization and immunogenicity of the CagF protein of the cag pathogenicity island of Helicobacter pylori.
Seydel A, Tasca E, Berti D, Rappuoli R, Del Giudice G, Montecucco C. Seydel A, et al. Infect Immun. 2002 Nov;70(11):6468-70. doi: 10.1128/iai.70.11.6468-6470.2002. Infect Immun. 2002. PMID: 12379730 Free PMC article.
It has been shown that CagA, a major antigen in humans, is translocated to the host cell via a secretion system encoded by the cag PAI. ...
It has been shown that CagA, a major antigen in humans, is translocated to the host cell via a secretion system encoded by the …
The crystal structure of the superoxide dismutase from Helicobacter pylori reveals a structured C-terminal extension.
Esposito L, Seydel A, Aiello R, Sorrentino G, Cendron L, Zanotti G, Zagari A. Esposito L, et al. Among authors: seydel a. Biochim Biophys Acta. 2008 Nov;1784(11):1601-6. doi: 10.1016/j.bbapap.2008.04.024. Epub 2008 May 6. Biochim Biophys Acta. 2008. PMID: 18502213
Helicobacter pylori produces a single SOD (HpSOD) which contains iron. The structure of this antioxidant protein has been determined at 2.4 A resolution. ...This structure provides a model for closely related sequences from species such as Campylobacter, wher …
Helicobacter pylori produces a single SOD (HpSOD) which contains iron. The structure of this antioxidant protein has been determined …
ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear.
Anselmi F, Hernandez VH, Crispino G, Seydel A, Ortolano S, Roper SD, Kessaris N, Richardson W, Rickheit G, Filippov MA, Monyer H, Mammano F. Anselmi F, et al. Among authors: seydel a. Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18770-5. doi: 10.1073/pnas.0800793105. Epub 2008 Dec 1. Proc Natl Acad Sci U S A. 2008. PMID: 19047635 Free PMC article.
A companion paper demonstrates that, if expression of either Cx26 or Cx30 is blocked, expression of the other is markedly down-regulated in the outer sulcus. Lanthanum, a connexin hemichannel blocker that does not affect gap junction (GJ) channels when applied extra
A companion paper demonstrates that, if expression of either Cx26 or Cx30 is blocked, expression of the other is markedly down-regula
Oxidative damage and autophagy in the human trabecular meshwork as related with ageing.
Pulliero A, Seydel A, Camoirano A, Saccà SC, Sandri M, Izzotti A. Pulliero A, et al. Among authors: seydel a. PLoS One. 2014 Jun 19;9(6):e98106. doi: 10.1371/journal.pone.0098106. eCollection 2014. PLoS One. 2014. PMID: 24945152 Free PMC article.
Autophagy also plays a major role in ocular patho-physiology. Molecular aging does occur in the trabecular meshwork, the main regulator of aqueous humor outflow, and trabecular meshwork senescence is accompanied by increased oxidative stress. ...In a subset of 10 su …
Autophagy also plays a major role in ocular patho-physiology. Molecular aging does occur in the trabecular meshwork, the main regulat …
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