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In-gel digestion for mass spectrometric characterization of proteins and proteomes.
Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M. Shevchenko A, et al. Nat Protoc. 2006;1(6):2856-60. doi: 10.1038/nprot.2006.468. Nat Protoc. 2006. PMID: 17406544
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-ge …
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-yea …
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